Abstract: This document describes biochemical pathways for producing pimelic acid, 7-hydroxyheptanoic acid, 7-aminoheptanoic acid, heptamethylenediamine or 1,7-heptanediol by forming two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C7 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on the CoA-dependent elongation enzymes or analog enzymes associated with the carbon storage pathways from polyhydroxyalkanoate accumulating bacteria.
Type:
Application
Filed:
December 13, 2013
Publication date:
September 4, 2014
Applicant:
INVISTA North America S.á r.I.
Inventors:
Adriana Leonora Botes, Alex Van Eck Conradie
Abstract: This document describes biochemical pathways for producing pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine or 1,7-heptanediol by forming two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C7 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on the carbon chain elongation enzymes or homologs thereof associated with the cyclohexane carboxylate biosynthesis from Syntrophus aciditrophicus or 2-aminoadipate lysine biosynthesis.
Type:
Application
Filed:
December 23, 2013
Publication date:
July 10, 2014
Applicant:
INVISTA North America S.á r.I.
Inventors:
Adriana Leonora Botes, Alex Van Eck Conradie, Changlin Chen, Paul S. Pearlman
Abstract: This document describes biochemical pathways for producing pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine or 1,7-heptanediol by forming one or two terminal functional groups, each comprised of carboxyl, amine or hydroxyl group, in a C7 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on the C1 elongation enzymes or homolog associated with coenzyme B biosynthesis.
Type:
Application
Filed:
December 23, 2013
Publication date:
July 10, 2014
Applicant:
INVISTA North America S.á r.I.
Inventors:
Adriana Leonora Botes, Alex Van Eck Conradie, Changlin Chen, Paul S. Pearlman
Abstract: This document describes biochemical pathways for producing pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine or 1,7-heptanediol by forming two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C7 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on the fatty acid synthesis pathway and oxidative cleavage of long chain acyl-[acp] intermediates by a monooxgenase (e.g., cytochrome P450) such as that encoded by BioI from microorganisms such as Bacillus subtillis.
Type:
Application
Filed:
December 23, 2013
Publication date:
July 10, 2014
Applicant:
INVISTA North America S.á r.I.
Inventors:
Adriana Leonora Botes, Alex Van Eck Conradie, Changlin Chen, Paul S. Pearlman