Abstract: The present disclosure provides methods of analysing the nucleotide read sequences of a nucleic acid sample of interest using high throughput bidirectional sequencing. The methods of the present disclosure are designed to work even where bidirectional sequencing produces forward and reverse reads that are not of a sufficient read length to be paired via the complementary hybridisation of overlapping sequences at the 3° end of the sequence reads. The disclosure further provides computer-implemented methods, computer-readable storage mediums and devices that implement a method for preparing nucleic acid sequence results for analysis from non-overlapping sequence reads for screening a nucleic acid sample of interest for the expression of one or more target nucleotide sequences.