Abstract: A method of culturing cells or tissue comprises: (a) disposing cells or tissue in the cavity of a deformable cell culture container; and (b) applying synchronized chronic electrical stimulation and stretch to the cells or tissue in the deformable culture cell container, wherein the synchronized chronic electrical stimulation is applied in the form of pulses at a frequency of from 0.010-99 Hz and a duration of from 0.4 to 24 ms; and wherein stretch cycles are applied to the cells or tissue at a frequency of from 0.010 to 15 Hz.
Type:
Grant
Filed:
March 2, 2017
Date of Patent:
September 24, 2019
Assignee:
IONOPTIX LLC
Inventors:
Peter G. W. Lee, Douglas L. Tillotson, Richard T. Udale, Katherine L. Barber
Abstract: A cell grabber has a unitary structure including a transparent grabber head at a terminus thereof and an elongated mounting portion extending therefrom for attachment to a force transducer. The grabber head has a planar or concave bonding surface which is coated with an adhesive film to secure the specimen, especially single cells, for tensile measurements. The cell grabber may be used in connection with auxotonic, isometric or isotonic force measurement systems.
Abstract: An apparatus that provides synchronized chronic electrical stimulation and chronic stretch to a cell culture or tissue. The apparatus includes a programmable controller which synchronizes stretch and electrical stimulation applied to a deformable culture container, such as a silicone rubber culture dish. The apparatus is used to treat cells and retard de-differentiation or promote differentiation and maturation in some applications.
Type:
Grant
Filed:
October 1, 2013
Date of Patent:
April 11, 2017
Assignee:
IONOPTIX LLC
Inventors:
Peter G. W. Lee, Douglas L. Tillotson, Richard T. Udale, Katherine L. Barber
Abstract: A cell grabber has a unitary structure including a transparent grabber head at a terminus thereof and an elongated mounting portion extending therefrom for attachment to a force transducer. The grabber head has a planar or concave bonding surface which is coated with an adhesive film to secure the specimen, especially single cells, for tensile measurements. The cell grabber may be used in connection with auxotonic, isometric or isotonic force measurement systems.
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