Abstract: The present invention provides a sperm sorting chip and a method for sorting sperm using the same. Said sperm sorting chip includes: a flow channel structure sequentially configured with a gradually diverging flow field region, a main flow channel, and a gradually converging main flow channel intercommunicated with each other from a first side end to a second side end; a fluid injection port, a semen injection port, and a semen extraction port separately located at the first side end and communicated with a main input channel of the gradual diverging flow field region; and a waste fluid outlet located at the second side end and communicated with the gradually converging main flow channel. The gradually diverging flow field region further includes a plurality of sub-input channels derived from the main input channel and converged into the main flow channel, and the plurality of sub-input channels have a gradually widening channel width at the junction with the main flow channel.