Abstract: Disclosed are a heating culture device used to provide an environment with a vertical temperature difference and a method of using the heating culture device. The heating culture device is provided for accommodating and heating a cell separation device which carries living cells having flagella and capable of moving autonomously and has a film for passing through the living cells and partitioning the cell separating device into upper and lower chambers. The heating culture device includes a carrying assembly, a chip carrier box, a case assembly and a control assembly. When a cover of the heating culture device is covered during operation, the control assembly can control driving state of an upper electric heating plate at the cover according to the temperature of a heat spreader set on the cover; and control a fan at the bottom according to the temperature at the bottom of the chip carrier box.

Abstract: The present invention provides a sperm sorting chip and a method for sorting sperm using the same. Said sperm sorting chip includes: a flow channel structure sequentially configured with a gradually diverging flow field region, a main flow channel, and a gradually converging main flow channel intercommunicated with each other from a first side end to a second side end; a fluid injection port, a semen injection port, and a semen extraction port separately located at the first side end and communicated with a main input channel of the gradual diverging flow field region; and a waste fluid outlet located at the second side end and communicated with the gradually converging main flow channel. The gradually diverging flow field region further includes a plurality of sub-input channels derived from the main input channel and converged into the main flow channel, and the plurality of sub-input channels have a gradually widening channel width at the junction with the main flow channel.