Abstract: Provided are a marker gene for identifying and classifying dopaminergic precursor cells (DPC), a screening method therefor and use thereof. The marker gene is a combination of 12 genes having specific up-regulation expression in the DPC cells relative to the neural stem cells (NSCs). The screening of the marker gene is based on a high-throughput RNA sequencing technology. In the samples of the NSC cells and the DPC cells, genes of which the specific expression is significantly up-regulated in the DPC cells are detected, and by bioinformatic analysis, a group of candidate genes capable of regulating the differentiation process of neural stem cells to dopaminergic neurons are identified. The marker gene combinations provided can be used to identify and classify DPC cell products for clinical treatment and to control the quality of the DPC cell products for clinical application.

Abstract: Use of a TGF-? small molecule inhibitor in the field of nerve regeneration, for in vitro regeneration and directed differentiation of a variety of nerve cells and brain-like organs. By adding the TGF-? small molecule inhibitor to a set of basal media having clear chemical compositions, pluripotent stem cells can be induced into adult cells derived from a variety of neural stem cells, and the number of induced nerve cells can be greatly increased.

Abstract: A chemical culture system and use thereof. The chemical culture system comprises a basic culture medium and a small molecule compound, and forms a serum-free culture system having a clear chemical composition. The culture medium does not use substitute serum B27 and GS21 which contain animal-derived components and are widely used currently, but uses a pure chemical molecule activation signal pathway, and uses a non-animal-derived growth factor to replace an animal-derived growth factor, and thus the composition is clear, the potential risks caused by the existence of serum and animal-derived components are avoided, and the clinical prospect of nerve cell transplantation is expanded.

Abstract: Provided is a new use of a TGF-? small molecule inhibitor in the field of neuroregeneration, which can be used for the in vitro regeneration and directed differentiation of various nerve cells and brain-like organs. By adding same to a set of basal media having clear chemical compositions. pluripotent stem cells can be induced into adult cells derived from a variety of neural stem cells, and the number of induced nerve cells and the size of organoids can be greatly increased. The induction system provided in the present invention expands new functions of a single small molecule in the field of ectodermal cell induction and differentiation and at the same time avoids the use of B27 and other serum substitutes, thereby completely avoiding the potential risks caused by the presence of animal-derived components in cell culture processes, and greatly expanding the clinical prospects of a variety of nerve cell transplantations.