Abstract: A method of reconstituting a powdered cell culture media includes providing a mixing apparatus and providing a powdered cell culture media to the fluid chamber of the mixing apparatus, prior to introducing fluid to the chamber. The method further includes introducing fluid to the chamber through an influent port, wherein the influent port is tangentially oriented relative to an inner wall of the fluid chamber to thereby cause the fluid to follow the wall of the fluid chamber in a circular motion, creating a vortex flow in the fluid chamber by introducing fluid at a sufficient flow rate, and enhancing the vortex flow with a geometric fluid flow aid by further channeling the water around the wall of the fluid chamber, around the geometric flow aid. The method further includes continuing to introduce fluid to the chamber and collecting reconstituted fluid that exits the chamber through the effluent port.

Abstract: An automated method includes providing a dry ingredient to be reconstituted into a liquid bioprocess solution and controlling, by a processing circuit, all automated system including at least one mixing chamber, an array of tubing for fluid flow within the system, and a plurality of valves provided within the tubing, to automatically prepare the liquid bioprocess solution from the dry ingredient. Controlling the auto system may include performing a series of sequential mixing steps, the series of sequential mixing steps causing the preparation of the liquid bioprocess solution. The method mixing include taking one or more measurements during the preparation of the liquid bioprocess solution, wherein each step is triggered by at least one of a measurement decreasing below, equaling, or exceeding a measurement threshold. Each step may also include opening or closing, by the processing circuit, at least one of the plurality of valves.

Abstract: A mixing apparatus for reconstituting a powdered cell culture media. The apparatus includes at least one fluid chamber, an influent port at a lower portion of the fluid chamber. The apparatus further includes a geometric fluid flow aid positioned in the fluid chamber and an effluent port at the top of the fluid chamber. A powdered cell culture media is provided in the fluid to be mixed with a fluid provided by the influent port. The effluent port is configured to allow reconstituted media to exit the fluid chamber.

Abstract: Disclosed is an adapted Madin-Darby canine kidney cell line capable of suspension culture in the absence of serum, and a chemically-defined medium for culture of the adapted MDCK cell line. Further disclosed are culture methods for growing the adapted MDCK cell line and methods for producing a vaccine from the adapted MDCK cell line grown in the chemically-defined medium.

Abstract: A mixing apparatus for reconstituting a powdered cell culture media. The apparatus includes at least one fluid chamber, an influent port at a lower portion of the fluid chamber. The apparatus further includes a geometric fluid flow aid positioned in the fluid chamber and an effluent port at the top of the fluid chamber. A powdered cell culture media is provided in the fluid to be mixed with a fluid provided by the influent port. The effluent port is configured to allow reconstituted media to exit the fluid chamber.

Abstract: Improved methods of culturing embryos in media having amounts of lactate that have not previously been recognized as beneficial for embryo development. Also, compositions, devices and kits related to the same.