Patents Assigned to ISEAO Technologies Limited
-
Patent number: 8080375Abstract: An improved method of detecting an enzyme in a sample, which enzyme is capable of adding or removing a chemical moiety to or from a nucleic acid molecule, thereby conferring the nucleic acid molecule with the ability to be extended to generate a novel detectable nucleic acid molecule, comprises the steps of allowing the sample to be tested for the presence of the enzyme to interact with the nucleic acid molecule; and testing for interaction of the enzyme with the nucleic acid molecule by detecting the novel nucleic acid molecule generated only in the presence of the enzyme. The preferred enzyme is a phosphatase. The methods have a number of applications, for example in enhancing the sensitivity of immunoassays, for detecting pathogen associated phosphatase, for diagnosing certain conditions and for detecting specific contaminants in a sample.Type: GrantFiled: May 18, 2006Date of Patent: December 20, 2011Assignee: Iseao Technologies LimitedInventors: Stuart Wilson, John Christopher Stanley, Sharon Banin
-
Publication number: 20110212438Abstract: Kits and devices for performing a method of detecting a molecule associated with viability of one or more cells or organisms in a sample are provided. The method comprises the initial step of contacting the sample with an enzyme, which enzyme is capable of adding or removing a chemical moiety to or from a nucleic acid molecule in the presence of the molecule associated with viability of the one or more cells or organisms. This thereby generates a novel detectable nucleic acid molecule. The next step involves detecting the presence of the molecule associated with viability of the one or more cells or organisms by detecting the novel nucleic acid molecule generated only in the presence of the molecule associated with viability of the one or more cells or organisms. A most preferred molecule associated with viability is ATP, although NAD may also be detected. A preferred enzyme for use in the methods is ligase.Type: ApplicationFiled: March 24, 2011Publication date: September 1, 2011Applicant: ISEAO TECHNOLOGIES LIMITEDInventors: Stuart Wilson, Sharon Banin
-
Patent number: 7943314Abstract: A method of detecting a molecule associated with viability of one or more cells or organisms in a sample comprises the initial step of contacting the sample with an enzyme, which enzyme is capable of adding or removing a chemical moiety to or from a nucleic acid molecule in the presence of the molecule associated with viability of the of the one or more cells or organisms. This thereby generates a novel detectable nucleic acid molecule. The next step involves detecting the presence of the molecule associated with viability of the one or more cells or organisms by detecting the novel nucleic acid molecule generated only in the presence of the molecule associated with viability of the one or more cells or organisms. A most preferred molecule associated with viability is ATP, although NAD may also be detected. A preferred enzyme for use in the methods is ligase.Type: GrantFiled: July 3, 2006Date of Patent: May 17, 2011Assignee: ISEAO Technologies LimitedInventors: Stuart Wilson, Sharon Banin
-
Publication number: 20090215047Abstract: A method of detecting a molecule associated with viability of one or more cells or organisms in a sample comprises the initial step of contacting the sample with an enzyme, which enzyme is capable of adding or removing a chemical moiety to or from a nucleic acid molecule in the presence of the molecule associated with viability of the of the one or more cells or organisms. This thereby generates a novel detectable nucleic acid molecule. The next step involves detecting the presence of the molecule associated with viability of the one or more cells or organisms by detecting the novel nucleic acid molecule generated only in the presence of the molecule associated with viability of the one or more cells or organisms. A most preferred molecule associated with viability is ATP, although NAD may also be detected. A preferred enzyme for use in the methods is ligase.Type: ApplicationFiled: July 3, 2006Publication date: August 27, 2009Applicant: ISEAO TECHNOLOGIES LIMITEDInventors: Stuart Wilson, Sharon Banin
-
Publication number: 20090098539Abstract: An improved method of detecting an enzyme in a sample, which enzyme is capable of adding or removing a chemical moiety to or from a nucleic acid molecule, thereby conferring the nucleic acid molecule with the ability to be extended to generate a novel detectable nucleic acid molecule, comprises the steps of allowing the sample to be tested for the presence of the enzyme to interact with the nucleic acid molecule; and testing for interaction of the enzyme with the nucleic acid molecule by detecting the novel nucleic acid molecule generated only in the presence of the enzyme. The preferred enzyme is a phosphatase. The methods have a number of applications, for example in enhancing the sensitivity of immunoassays, for detecting pathogen associated phosphatase, for diagnosing certain conditions and for detecting specific contaminants in a sample.Type: ApplicationFiled: May 18, 2006Publication date: April 16, 2009Applicant: ISEAO TECHNOLOGIES LIMITEDInventors: Stuart Wilson, John Christopher Stanley, Sharon Banin
-
Publication number: 20040259131Abstract: The invention relates to methods of detecting target molecules or the interactions between target molecules based on interactions between proximally-bound nucleic acid tags. Reagent kits for use in such methods are also provided.Type: ApplicationFiled: April 2, 2004Publication date: December 23, 2004Applicant: Iseao Technologies LimitedInventors: Stuart Wilson, Christopher John Stanley