Patents Assigned to IsoPlexis Corporation
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Patent number: 11940413Abstract: The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. A plurality of smaller flow cells is employed, each with a relatively small area to be imaged, in order to provide greater flexibility and efficiency.Type: GrantFiled: March 8, 2021Date of Patent: March 26, 2024Assignee: IsoPlexis CorporationInventors: Steven Gordon, Thomas Hagerott, Edmund Golaski, Jerzy Olejnik
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Patent number: 11866741Abstract: The present invention relates to a polymerase enzyme from 9° N with improved ability to incorporate reversibly terminating nucleotides. The enzyme comprising mutations in the motif A region. The invention also relates to methods of using such enzymes as well as a kit with such polymerases.Type: GrantFiled: June 17, 2021Date of Patent: January 9, 2024Assignee: IsoPlexis CorporationInventors: Jerzy Olejnik, Angela Delucia
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Patent number: 11821032Abstract: The present invention provides methods, compositions, mixtures and kits utilizing 5-Chloro-2-methyl-4-isothiazolin-3-one in sequencing reactions, and in particular, sequencing reactions where deoxynucleoside triphosphates comprising a 3?-O position capped by a disulfide-based 3?-terminator group are used. In one embodiment, the deoxynucleoside triphosphates comprise a 3?-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. In addition, thiol-containing compounds and scavengers of thio-containing compounds are described. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.Type: GrantFiled: August 21, 2020Date of Patent: November 21, 2023Assignee: IsoPlexis CorporationInventors: Luisa Andruzzi, Jimmy Adediran, Timothy Pelletier, Austin Ricker, Angela DeLucia, Ben McNally, Dona Hevroni, Mong Sano Marma, John Andrew Sheridan
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Patent number: 11661619Abstract: Embodiments disclose apparatus, methods and software for performing biological screening and analysis implemented using an instrument platform capable of detecting a wide variety of cell-based secretions, expressed proteins, and other cellular components. The platform may be configured for simultaneous multiplexed detection of a plurality biological components such that a large number of discrete samples may be individually sequestered and evaluated to detect or identify constituents from the samples in a highly parallelized and scalable manner.Type: GrantFiled: June 10, 2021Date of Patent: May 30, 2023Assignee: IsoPlexis CorporationInventors: Patrick Paczkowski, Sean Mackay, Sean McCusker
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Patent number: 11548909Abstract: The present invention provides methods, compositions, mixtures and kits utilizing deoxynucleoside triphosphates comprising a 3?-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.Type: GrantFiled: March 22, 2018Date of Patent: January 10, 2023Assignee: IsoPlexis CorporationInventors: Mong Sano Marma, Jerzy Olejnik, Ilia Korboukh
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Patent number: 11525783Abstract: Embodiments of the current disclosure are directed to systems, methods and apparatus for evaluating single cell secretion profiles. In some embodiments, the apparatus may be configured to analyze substances expressed by a biological cell and may include a first compressible substrate, and a second substrate configured for removable sealing attachment with the first substrate. In some embodiments, upon attachment of the second substrate with the first substrate, an assembly is formed such that the open side of the plurality of chambers are covered by the second substrate, and a portion of each of the plurality of capture areas are exposed in each of the chambers.Type: GrantFiled: November 22, 2017Date of Patent: December 13, 2022Assignee: IsoPlexis CorporationInventors: Peter Tsiomplikas, Jerry Sedgewick, Jeffrey Whynall, Sean MacKay, Colin Ng, Patrick Paczkowski, Sean McCusker, Igor Nikonorov, Alaina Kaiser
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Patent number: 11493508Abstract: Disclosed are compositions and methods for the multiplexed analysis of one or more intracellular targets of a single cell. Exemplary compositions of the disclosure comprise a surface comprising a plurality of capture agents operatively-linked thereto, wherein each capture agent specifically binds to a distinct intracellular target and wherein the plurality of capture agents form a repeating pattern; a substrate comprising a plurality of chambers, wherein the substrate releasably couples with the surface and wherein each chamber of the plurality of chambers comprises at least one repeat of the repeating pattern of the plurality of capture agents of the surface; a coating composition comprising a cell lysis composition; and a linker composition comprising a functionalization component and an extension component.Type: GrantFiled: November 13, 2017Date of Patent: November 8, 2022Assignee: IsoPlexis CorporationInventors: Colin Ng, Alaina Kaiser, Emily Bettini, Patrick Paczkowski, Sean Mackay, Feimei Liu, Maithreyan Srinivasan
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Patent number: 11421264Abstract: The present invention provides methods, compositions, mixtures and kits utilizing deoxynucleoside triphosphates comprising a 3?-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. In addition, thiol-containing compounds and scavengers of thio-containing compounds are described. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.Type: GrantFiled: March 13, 2017Date of Patent: August 23, 2022Assignee: IsoPlexis CorporationInventors: Mong Sano Marma, Luisa Andruzzi, Ben McNally, Angela DeLucia, Dona Hevroni, Elizabeth Carpenter, Magnus Besev
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Patent number: 11162129Abstract: The invention relates to methods, compositions, devices, systems and kits as described including, without limitation, reagents and mixtures for determining the identity of nucleic acids in nucleotide sequences using, for example, sequencing by synthesis methods. In particular, the present invention contemplates the use of photoprotective mixture of compounds as imaging reagents to improve stability and storage of fluorescent compounds, including but not limited to, nucleotides with fluorescent labels.Type: GrantFiled: October 31, 2017Date of Patent: November 2, 2021Assignee: IsoPlexis CorporationInventors: Luisa Andruzzi, Michel Georges Perbost, Dona Hevroni, Minakshi Guha, Austin Ricker, Timothy Pelletier
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Patent number: 11149309Abstract: The present invention relates to methods and devices for amplifying nucleic acid, and, in particular, amplifying so as to generate products on a surface without the use of emulsions. In a preferred embodiment, a plurality of groups of amplified product are generated on the surface, each group positioned in different (typically predetermined) locations on said surface so as to create an array.Type: GrantFiled: September 5, 2017Date of Patent: October 19, 2021Assignee: IsoPlexis CorporationInventors: Steven Gordon, Daniel Stetson
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Patent number: 11117921Abstract: The present invention provides methods, compositions, mixtures and kits utilizing deoxynucleoside triphosphates comprising a 3?-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.Type: GrantFiled: May 28, 2019Date of Patent: September 14, 2021Assignee: IsoPlexis CorporationInventors: Mong Sano Marma, Jerzy Olejnik, Ilia Korboukh
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Patent number: 11078526Abstract: The invention relates to methods, compositions, devices, systems and kits as described including, without limitation, reagents and mixtures for determining the identity of nucleic acids in nucleotide sequences using, for example, sequencing by synthesis methods. In particular, the present invention contemplates the use of polyphenolic compounds, known as antioxidant additives, to improve the efficiency of Sequencing-By-Synthesis reactions. For example, gallic acid (GA) is shown herein to be one of many exemplary SBS polyphenolic additives.Type: GrantFiled: May 24, 2019Date of Patent: August 3, 2021Assignee: IsoPlexis CorporationInventors: Jerzy Olejnik, Michel Georges Perbost
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Patent number: 11066689Abstract: Embodiments disclose apparatus, methods and software for performing biological screening and analysis implemented using an instrument platform capable of detecting a wide variety of cell-based secretions, expressed proteins, and other cellular components. The platform may be configured for simultaneous multiplexed detection of a plurality biological components such that a large number of discrete samples may be individually sequestered and evaluated to detect or identify constituents from the samples in a highly parallelized and scalable manner.Type: GrantFiled: January 27, 2020Date of Patent: July 20, 2021Assignee: IsoPlexis CorporationInventors: Patrick Paczkowski, Sean MacKay, Sean McCusker
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Patent number: 10584366Abstract: Embodiments disclose apparatus, methods and software for performing biological screening and analysis implemented using an instrument platform capable of detecting a wide variety of cell-based secretions, expressed proteins, and other cellular components. The platform may be configured for simultaneous multiplexed detection of a plurality biological components such that a large number of discrete samples may be individually sequestered and evaluated to detect or identify constituents from the samples in a highly parallelized and scalable manner.Type: GrantFiled: December 3, 2015Date of Patent: March 10, 2020Assignee: IsoPlexis CorporationInventors: Patrick Paczkowski, Sean MacKay, Sean McCusker
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Publication number: 20190376898Abstract: Embodiments of the current disclosure are directed to systems, methods and apparatus for evaluating single cell secretion profiles. In some embodiments, the apparatus may be configured to analyze substances expressed by a biological cell and may include a first compressible substrate, and a second substrate configured for removable sealing attachment with the first substrate. In some embodiments, upon attachment of the second substrate with the first substrate, an assembly is formed such that the open side of the plurality of chambers are covered by the second substrate, and a portion of each of the plurality of capture areas are exposed in each of the chambers.Type: ApplicationFiled: November 22, 2017Publication date: December 12, 2019Applicant: IsoPlexis CorporationInventors: Peter TSIOMPLIKAS, Jerry SEDGEWICK, Jeffrey WHYNALL, Sean MACKAY, Colin NG, Patrick PACZKOWSKI, Sean MCCUSKER, Igor NIKONOROV, Alaina KAISER
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Publication number: 20190285626Abstract: Disclosed are compositions and methods for the multiplexed analysis of one or more intracellular targets of a single cell. Exemplary compositions of the disclosure comprise a surface comprising a plurality of capture agents operatively-linked thereto, wherein each capture agent specifically binds to a distinct intracellular target and wherein the plurality of capture agents form a repeating pattern; a substrate comprising a plurality of chambers, wherein the substrate releasably couples with the surface and wherein each chamber of the plurality of chambers comprises at least one repeat of the repeating pattern of the plurality of capture agents of the surface; a coating composition comprising a cell lysis composition; and a linker composition comprising a functionalization component and an extension component.Type: ApplicationFiled: November 13, 2017Publication date: September 19, 2019Applicant: IsoPlexis CorporationInventors: Colin NG, Alaina KAISER, Emily BETTINI, Patrick PACZKOWSKI, Sean MACKAY, Feimei LIU, Maithreyan SRINIVASAN