Abstract: A method and test kit provide for the cost-effective and resource-saving extraction of nucleic acids. “Resource-saving” is to be understood as largely avoiding plastic waste. The method includes adding a binding buffer to a nucleic acid-containing solution where the nucleic acids are bound to a solid phase, washing the bound nucleic acids, and eluting the washed nucleic acids. A reaction vessel having grooves on or a thread cut into its inner side is used as the solid phase. Before the nucleic acids are bound, they can be released from cells by means of lysis. The test kit includes at least one reaction vessel as described, at least one binding buffer for binding the nucleic acids to the inner side of the reaction vessel, at least one wash buffer for washing the bound nucleic acids, at least one elution buffer for eluting the washed nucleic acids and optionally lysis buffers.

Abstract: The invention relates to magnetically separable polymer-based macro granules for carrying out immunoassays for detecting highly diverse analytes for medical, biological and biotechnological sectors. The size of the macroscopic granulate particles is between 0.5 mm and 10 mm in cross-section, preferably between 1 mm and 5 mm. Preferably, the macro granules can be magnetically separated. According to a preferred embodiment, the polymer-based macro granules are located in a pipette tip.

Abstract: The invention relates to a simple and very rapid method for simultaneously isolating nucleic acids from sedimentable and nonsedimentable biomolecules in water samples. The water samples can be, inter alia, drinking water, industrial water, surface water or else wastewater. The biomolecules are bacteria, bacteriophages, protozoa, viruses, and free-circulating nucleic acids (e.g., plasmid DNA) encoding antibiotic resistances. According to the invention, a polysaccharide derivative is added to the water sample and the biomolecules am thus enriched. After dissolution of the pellet and lysis of the biomolecules, the nucleic acids are bound to a solid phase, purified and eluted in a known manner.

Abstract: Biological cells are concentrated and isolated from a sample and/or biological cells are concentrated from a sample, followed by the isolation of nucleic acids from said cells. The sample is brought into contact with a solid phase which has a rough or structured surface.

Abstract: A method includes enriching biomolecules and removal of the biomolecules from a biological sample. In the presence of particles, an alginate solution and salts of divalent and/or polyvalent cations or an acid are added to a biological sample, and an alginate-gel-biomolecule-complex is formed on the particles. The complex is removed from the sample by separation of the particles, and from which subsequently the biomolecules or ingredients of the biomolecules are released. The biomolecules, which shall be enriched, include cell-free nucleic acids, viruses or subcellular microparticles.

Abstract: The invention relates to a method for detecting various analytes, characterized by the following steps: a) providing separation particles containing, on their surface, firstly means of binding the analyte to be identified and secondly means of separating the analyte bound to the particles; b) providing identification particles firstly having, on their surface, means for binding the analyte to be identified and secondly containing on their surface or enclosed therein, means which are capable, after they have been detached or released from the particles, by virtue of their labeling, of generating a signal which serves for identification of the analyte; c) combining analyte, separation particles and identification particles; d) removing and washing the identification particles bound via the analyte by means of the separation particles; e) releasing the means which serve to identify the analyte, characterized in that the means which serve to identify the analyte are coupled reversibly to the identification partic

Abstract: A method and test kit are useful for detecting specific nucleic acid sequences. The process includes (1) matrix-dependent new synthesis of the target nucleic acid; (2) target-specific probe hybridization; and (3) detection of the hybridization event. In the first step, an oligonucleotide 1, which is marked by a marker 1 and is entirely or partially complementary to the target sequence, acts as a primer in the matrix-dependent new synthesis of the target nucleic acid and, in the second step, an oligonucleotide 2, which is marked by a marker 2 and, owing to its melting temperature being lower than that of the oligonucleotide 1, is not involved in the first step, partially or completely hybridizes with the DNA new synthesis product of oligonucleotide 1.