Abstract: The present invention relates to a regulation composition for gametophytic self-incompatibility which contains sulfates especially CuSO4 and ZnSO4 as an inhibitor, which prevents a style-specific RNase activity regulating gametophytic self-incompatibility; a control method for gametophytic self-incompatibility of a plant by using the regulation composition for gametophytic self-incompatibility; and a plant self-pollinated by destroying the gametophytic self-incompatibility, using the control method. By using the regulation composition for gametophytic self-incompatibility according to the present invention, a single species of fruit trees can be self-pollinated without cultivating other pollinizer, so that fruition rate can be increased and the productivity per unit area can be maximized.