Abstract: A method and an implementation are disclosed for measuring the contents of a certain component (e.g. a hormone) in a biological fluid. The basic principle of the method is that a complex is formed starting from the antagonist of the component sought for with the product of conjugation of the latter with a tracer (e.g. a radioisotope), said complex is introduced in the biological fluid to be tested and the quantity of the tracer which has been set free is measured with a measuring or dosing technique which is appropriate for the tracer which has been selected.
Type:
Grant
Filed:
May 30, 1980
Date of Patent:
June 14, 1983
Assignee:
Istituto Sieroterapico e Vaccinogeno Toscano "Sclavo" S.p.A.
Inventors:
Francesco Cocola, Paolo Tarli, Paolo Neri
Abstract: A novel composition is disclosed for determining the activity of the alkaline phosphatase, which comprises a salt of p-nitrophenylphosphoric acid, a magnesium salt, sodium borate and 2-amino-2-hydroxymethyl-1,3-propanediol. The composition affords advantages over the known composition especially as regards sensitivity.
Type:
Grant
Filed:
September 25, 1980
Date of Patent:
December 15, 1981
Assignee:
Istituto Sieroterapico E Vaccinogeno Toscano "SCLAVO" S.p.A.
Abstract: A method and a combination of reagents are disclosed for determining the creatine kinase activity by inhibiting the action of creatine kinase, the improvement consisting in that adenosine - 5'- monophosphate is used in combination with an inorganic, preferably an alkali metal, fluoride. By so doing the masking effect of adenylate kinase are efficiently offset.
Type:
Grant
Filed:
April 13, 1978
Date of Patent:
September 2, 1980
Assignee:
Istituto Sieroterapico e Vaccinogeno Toscano Sclavo S.p.A.
Inventors:
Franco Meiattini, Giuliano Giannini, Paolo Tarli
Abstract: A method for measuring the antistreptolysin concentration in a human blood sample by adding a solution of oxidized 0-streptolysin to the blood sample (diluted), incubating, reacting with a reducing solution, incubating and relating the resulting hemolysis to the antistreptolysin concentration.
Type:
Grant
Filed:
December 2, 1976
Date of Patent:
April 10, 1979
Assignee:
Istituto Sieroterapico e Vaccinogeno Toscano "Sclavo" S.p.A.
Abstract: Process for enzymatic determination of glucose with a glucose-oxidase-peroxidase enzyme system involving the use of a reduced acceptor which is oxidized. As reduced acceptor in the enzymatic reaction catalyzed by peroxidase, a ferrocyanide salt is oxidized to ferricyanide; then a further reducing system is introduced, which system is oxidized by the ferricyanide, with the formation of a colored quinone compound, the reaction between ferricyanide and the reducing system taking place spontaneously.
Type:
Grant
Filed:
March 2, 1976
Date of Patent:
December 20, 1977
Assignee:
Istituto Sieroterapico e Vaccinogeno Toscano "SCLAVO", S.p.A.
Abstract: A method for measuring the antistreptolysin concentration in a human blood sample by adding a solution of oxidized O-streptolysin to the blood sample (diluted), incubating, reacting with a reducing solution, incubating and relating the resulting hemolysis to the antistreptolysin concentration.
Type:
Grant
Filed:
April 8, 1981
Date of Patent:
September 6, 1983
Assignee:
Istituto Sieroterapico e Vaccinogeno Toscano "Sclavo" S.p.A.