Abstract: Contiguity information is important to achieving high-quality de novo assembly of mammalian genomes and the haplotype-resolved resequencing of human genomes. The methods described herein pursue cost-effective, massively parallel capture of contiguity information at different scales.

Abstract: Next Generation DNA sequencing promises to revolutionize clinical medicine and basic research. However, while this technology has the capacity to generate hundreds of billions of nucleotides of DNA sequence in a single experiment, the error rate of approximately 1% results in hundreds of millions of sequencing mistakes. These scattered errors can be tolerated in some applications but become extremely problematic when “deep sequencing” genetically heterogeneous mixtures, such as tumors or mixed microbial populations. To overcome limitations in sequencing accuracy, a method Duplex Consensus Sequencing (DCS) is provided. This approach greatly reduces errors by independently tagging and sequencing each of the two strands of a DNA duplex. As the two strands are complementary, true mutations are found at the same position in both strands. In contrast, PCR or sequencing errors will result in errors in only one strand.

Abstract: The present disclosure generally relates to the methods and compositions to efficiently analyze polymer characteristics using nanopore-based assays. Specifically disclosed is a method for generating reference signals for polymer analysis in a nanopore system, wherein the nanopore system has a multi-subunit output signal resolution. The method comprises translocating a reference sequence through a nanopore to generate a plurality of reference output signals, wherein each possible multi-subunit sequence that can determine an output signal appears only once in the reference sequence. The output signals are compiled into a reference map for nanopore analysis of an analyte polymer. Also provided are methods and compositions for calibrating the nanopore system for optimized polymer analysis.

Abstract: Provided herein, among other aspects, are methods and apparatuses for ranking aliquots from a suspension containing bioparticles. In certain embodiments, the bioparticles may be cells, organelles, proteins, DNAs, debris of biological origin, microbeads coated with biological compounds, or viral particles. As such, the methods and apparatuses provided herein may be used to quantify rare cells such as circulating cancer cells, fetal cells and other rare cells present in bodily fluids for disease diagnosis, prognosis, or treatment.

Abstract: A method for a device, including an integrity information collecting step S10 of measuring and collecting a time consumed from a start time of one operation to an end time in a normal state of a device; a defect information collecting step S20 of measuring and collecting a time consumed from a start time of one operation to an end time in a state of a device; a setting step S30 of setting an integrity reference value and a defect reference value for the time consumed for one operation of the device; a detecting step S40 of measuring and collecting a time value consumed from a start time of an operation of the device to an end time in real time and detecting an integrity index value of the device; and an outputting step S50 of outputting the integrity index value.

Abstract: Next Generation DNA sequencing promises to revolutionize clinical medicine and basic research. However, while this technology has the capacity to generate hundreds of billions of nucleotides of DNA sequence in a single experiment, the error rate of approximately 1% results in hundreds of millions of sequencing mistakes. These scattered errors can be tolerated in some applications but become extremely problematic when “deep sequencing” genetically heterogeneous mixtures, such as tumors or mixed microbial populations. To overcome limitations in sequencing accuracy, a method Duplex Consensus Sequencing (DCS) is provided. This approach greatly reduces errors by independently tagging and sequencing each of the two strands of a DNA duplex. As the two strands are complementary, true mutations are found at the same position in both strands. In contrast, PCR or sequencing errors will result in errors in only one strand.

Abstract: The present disclosure generally relates to the methods and compositions to efficiently analyze polymer characteristics using nanopore-based assays. Specifically disclosed is a method for generating reference signals for polymer analysis in a nanopore system, wherein the nanopore system has a multi-subunit output signal resolution. The method comprises translocating a reference sequence through a nanopore to generate a plurality of reference output signals, wherein each possible multi-subunit sequence that can determine an output signal appears only once in the reference sequence. The output signals are compiled into a reference map for nanopore analysis of an analyte polymer. Also provided are methods and compositions for calibrating the nanopore system for optimized polymer analysis.

Abstract: Methods, devices, and systems for performing digital assays are provided. In certain aspects, the methods, devices, and systems can be used for the amplification and detection of nucleic acids. In certain aspects, the methods, devices, and systems can be used for the recognition, detection, and sizing of droplets in a volume. Also provided are compositions and kits suitable for use with the methods and devices of the present disclosure.

Abstract: A boom gate including: a pedestal locatable on a surface and an elongate arm to extend across an area to be temporarily blocked; the elongate arm having a free end and a fixed end, the fixed end being pivotably attached to the pedestal and driven by a drive mechanism to permit the arm to move between a generally horizontal position and a generally vertical position, preventing or allowing access past the gate, respectively; the arm including a first elongate portion defining a first horizontal axis and a second portion defining a second horizontal axis, the first and second axes being spaced away from each other to permit the gate to be used in combination with a traffic management barrier.

Abstract: The present invention relates to an integrity index detecting method for a device by means of a control output signal which, after establishing an integrity index reference table based on an integrity reference value set based on information collected from a normal device and a defect reference value set based on information collected from a device before a malfunction occurs, outputs an integrity index value indicating an integrity of the device in real time by applying a gradient value with respect to a time interval value between control output signals collected from the device in real time to the integrity index reference table to provide the integrity index value to a manager.

Abstract: A payment processing method uses a credit push for disbursing funds at ATM. The process can include scanning an optical code at an ATM using a mobile device to determine creditor bank information. A debtor bank server receives a funds disbursement request and provides a credit push request to a payment rail. The payment rail communicates a credit payment transaction request to a creditor bank sever. The creditor bank server identifies the ATM using an ATM identifier provided to the mobile device via the optical code. The ATM is instructed to disburse funds by the creditor bank server. The method includes using the mobile device, or other issuer domain, authentication procedures. The funds can be disbursed by the ATM without the ATM performing any authentication procedures.

Abstract: The present invention provides, among other aspects, stabilized chromophoric nanoparticles. In certain embodiments, the chromophoric nanoparticles provided herein are rationally functionalized with a pre-determined number of functional groups. In certain embodiments, the stable chromophoric nanoparticles provided herein are modified with a low density of functional groups. In yet other embodiments, the chromophoric nanoparticles provided herein are conjugated to one or more molecules. Also provided herein are methods for making rationally functionalized chromophoric nanoparticles.

Abstract: The present invention provides a method of predictively maintaining an elevator driving unit which distinguishes between a condition when moving an elevator upward and a condition when moving the elevator downward, collects driving information of the driving unit (information on a change over time in current values) in a normal state, collects driving information of the driving unit before the occurrence of breakdown, sets critical levels based on the collected information, detects in real time an abnormal symptom of the driving unit by comparing the driving information of the driving unit, which is collected in real time, with the set critical level, and then performs stable predictive maintenance of the elevator driving unit, thereby efficiently preventing a safety accident of the elevator caused by a breakdown of the elevator driving unit.

Abstract: The invention relates to a measuring system for determining a dispensed amount of hydrogen of a hydrogen dispensing location from a hydrogen dispensing unit present there to a receiving tank including a measuring unit. The measuring unit can have a flowmeter, wherein the measuring system is designed to establish a fluid-tight connection between the hydrogen dispensing unit and the receiving tank. Furthermore, the flowmeter has an active cooling. The invention further relates to a measuring method for determining a dispensed amount of hydrogen.

Abstract: Provided herein, among other aspects, are methods and apparatuses for analyzing particles in a sample. In some aspects, the particles can be analytes, cells, nucleic acids, or proteins and contacted with a tag, partitioned into aliquots, detected by a ranking device, and isolated. The methods and apparatuses provided herein may include a microfluidic chip. In some aspects, the methods and apparatuses may be used to quantify rare particles in a sample, such as cancer cells and other rare cells for disease diagnosis, prognosis, or treatment.

Abstract: An adaptive system for efficient and long-range wireless power delivery using magnetically coupled resonators responds to changes in a dynamic environment, and maintains high efficiency over a narrow or fixed frequency range. The system uses adaptive impedance matching to maintain high efficiency. The wireless power transfer system includes a drive inductor coupled to a high-Q transmitter coil, and a load inductor coupled to a high-Q receiver coil. The transmitter coil and receiver coil for a magnetically coupled resonator. A first matching network is (i) operably coupled to the drive inductor and configured to selectively adjust the impedance between the drive inductor and the transmitter coil, or (ii) is operably coupled to the load inductor and configured to selectively adjust the impedance between the load inductor and the receiver coil.

Abstract: A method for predictive maintenance of equipment via a distribution chart is disclosed. Peak values are extracted based on a change in an amount of energy required for performing a work process by the equipment in a normal state, a distribution chart of the extracted peak values is constructed, and an abnormal symptom of the equipment is predictively detected in advance based on a change in distribution probability of a detection section having a low distribution probability and somewhat high risk in the constructed distribution chart thereof such that maintenance and replacement of the equipment are induced to be carried out at an appropriate time. Thus, an enormous monetary loss caused by a failure in the equipment may be prevented in advance.

Abstract: A method for learning and detecting an abnormal part of a device through artificial intelligence comprises: an information collection step for collecting a current waveform of a current value that changes over time in a driving state of at least one device and collecting information about a faulty part of the device, together with current waveform information before a fault occurs in the device; a model setting step for learning, by a control unit, information collected in the information collection step and setting a reference model of a current waveform for each faulty part of the device; and a detection step for, when an abnormal symptom of the device is detected in a real-time driving state, comparing, by the control unit, a real-time current waveform of the device and the reference model, and detecting and providing an abnormal part regarding the abnormal symptom of the device.

Abstract: Polymers, monomers, chromophoric polymer dots and related methods are provided. Highly fluorescent chromophoric polymer dots with narrow-band emissions are provided. Methods for synthesizing the chromophoric polymers, preparation methods for forming the chromophoric polymer dots, and biological applications using the unique properties of narrow-band emissions are also provided.

Abstract: A server rack predictive maintenance method comprises: an information collecting step of measuring and collecting the value of power consumed by a main wire for supplying power to a fan and pieces of equipment in a normal operating state of a server rack in which the internal temperature and humidity are maintained to be constant through the fan; an alarm setting step of setting the average current value of the server rack on the basis of the value of power collected in the information collecting step, and setting the alarm current value to be smaller than the set average current value; and a detecting step of detecting the server rack as an abnormal state when the value of power consumed by the main wire, having been measured in a real-time operating state of the server rack, is formed less than the alarm current value.