Abstract: Ribonucleoproteins and RNA-binding proteins related to the amino acid sequence corresponding at least to residues 92 to 202 and up to about residues 1 to about 240 of sequence (III), or of a modification of such sequences (III) having at least 35% homology with sequence (III) are disclosed.
Type:
Grant
Filed:
June 7, 1995
Date of Patent:
February 2, 1999
Assignees:
Jack D. Keene, Charles C. Query, Rex Bentley
Inventors:
Jack D. Keene, Charles C. Query, Rex O. Bentley
Abstract: Methods and compounds are described that allow partitioning of messenger RNAs encoding runctionally related proteins, in particular those involved in cellular growth and differentiation, based upon binding to target sequences in the untranslated portions of the RNAS. The preparation of mRNA-subset libraries containing genes encoding such growth regulatory factors is described together with novel gene sequences derived useing these mRNA partitioning methods. These methods of mRNA partitioning allow access to growth regulatory factors unique to a given cell, tissue or tumor and provide a method of fingerprinting such factors.
Type:
Grant
Filed:
June 7, 1995
Date of Patent:
June 30, 1998
Assignees:
Jack D. Keene, Todd Levine, FenBiao Gao
Inventors:
Jack D. Keene, Todd Levine, FenBiao Gao
Abstract: A peptide, Hel-N1, which can bind to a 3'-untranslated mRNA sequence (which encompasses the "instability sequence") that is uniquely present in the messenger RNAs that encode oncoproteins and lymphokines, and mediates the specific destruction of the messenger RNAs, is described. Full-length Hel-N1 is capable of suppressing cell growth and causing cellular differentiation. Hel-N1 possess three RNA recognition motifs. One of these forms an RNA-binding domain which, when transfected alone into cells, causes them to undergo rapid growth.
Type:
Grant
Filed:
June 6, 1995
Date of Patent:
December 16, 1997
Assignees:
Jack D. Keene, Peter H. King, Todd Levine
Inventors:
Jack D. Keene, Peter H. King, Todd Levine