Abstract: The present invention relates to an expression vector that encodes all or a portion of replicon proteins from a positive stranded virus, wherein expression of the replicon proteins is under the control of CMV and T7 promoters, and wherein expression of a payload is under the control of a sub-genomic promoter. Also provided are methods of using the vector in therapeutics and vaccines.

Abstract: The present invention relates to macromolecular complexes comprising micron-scale networks which include binding motifs thereon which allow the covalent bonding of the micron-scale networks to particles which provide nanoscale display surfaces. In particular the present invention relates to micron-scale networks of TMV coat proteins comprising a peptide tag (e.g. SpyTag) and particles providing a nanoscale display surface comprising GFP and a corresponding binding protein (e.g. SpyCatcher) wherein the peptide tag and binding protein pair are capable of spontaneously forming a covalent bond.

Abstract: There is provided herein controllable power and lighting arrangement suitable for use in commercial and/or domestic applications. There is particularly provided a method for the arrangement and automatic control of light emitting diode (LED) lights, and optionally non-LED based devices, powered by low voltage AC power distributed on bus bars. In addition there is provided a power and lighting arrangement which is especially suitable for use in a uniform and safe manner in close proximity to living organisms, particularly organisms capable of growth, such as plants, in a domestic or commercial growth system.

Abstract: There is provided a method of nucleic acid analysis which allows analysis of genetic diversity in multiple populations to be performed rapidly and simultaneously. The method comprises (a) isolating nucleic acid from said sample; (b) providing at least two pairs of labelled primers, wherein each said primer pair is complementary to a marker sequence in a nucleic acid of at least one member; (c) amplifying the nucleic acid; (d) digesting the labelled amplified nucleic acid with at least one restriction enzyme to produce restriction fragments, and size sorting said fragments to produce a restriction fragment length profile, and (e) analysing said restriction fragment length profile so obtained; wherein the primer pairs provided for each marker have a different sequence to the sequence of the primer pairs for each other marker, and wherein each said primer pair is uniquely labelled relative to the other primer pair(s). In one embodiment each primer pair is uniquely labelled at the 5? end with a fluorophore.

Abstract: A new cultivar of Ribes nigrum, ‘Ben Como’, characterized by its consistent high yields of fruit, its upright and compact growth habit, its high degree of fruit set, its resistance to white pine blister rust and its suitability for machine harvesting, and in producing a larger plant, smaller berry, and fruit that ripens later than ‘Ben Chaska’.

Abstract: A new cultivar of Ribes nigrum, ‘Ben Chaska’, characterized by its consistent high yields of fruit, its upright and compact growth habit, its high degree of fruit set, its resistance to white pine blister rust and its suitability for machine harvesting and in having a more compact plant habit, larger berries and earlier fruit ripening than ‘Ben Como’.