Abstract: Platforms for enzymatic assays for biomarkers, including systems, methods, and measuring devices by which a biomarker, such as creatinine, is measured using a small amount of biological fluid, such as blood, plasma, or serum. The measuring device or biosensor can be a test strip including a layered active component assembly positioned between two outer layers which enables multi-step enzymatic reactions operating in kinetic and/or endpoint (in which the reaction is allowed to near completion), and generally includes multiple layers with primary enzyme(s), coupling enzyme(s), and reagents to produce an optical signal correlated to the concentration of a biomarker in the sample. The test strip can be read using a portable optical reader coupled to a smart phone or tablet.

Abstract: Platforms for enzymatic assays for biomarkers, including systems, methods, and measuring devices by which a biomarker, such as creatinine, is measured using a small amount of biological fluid, such as blood, plasma, or serum. The measuring device or biosensor can be a test strip including a layered active component assembly positioned between two outer layers which enables multi-step enzymatic reactions operating in kinetic and/or endpoint (in which the reaction is allowed to near completion), and generally includes multiple layers with primary enzyme(s), coupling enzyme(s), and reagents to produce an optical signal correlated to the concentration of a biomarker in the sample. The test strip can be read using a portable optical reader coupled to a smart phone or tablet.

Abstract: A mobile device based multi-analyte testing analyzer for use in medical diagnostic monitoring and screening, and a method of manufacturing the same are disclosed. A reflectance based, colorimetric test strip reader for use with a mobile device having a jack plug receiving socket, said test strip reader adapted for removably receiving a test strip having a test strip longitudinal axis, comprising a housing; a jack plug operably coupled to and extending from said housing and adapted for operable coupling with said jack plug receiving socket; a test strip adapter including structure defining a test strip receiving channel; a light source oriented within said housing for directing light toward said test strip receiving channel to illuminate a test strip arranged within said test strip adapter; and a light sensor oriented within said housing to sense light reflected from a test strip carried by said test strip receiving channel.

Abstract: A mobile device based multi-analyte testing analyzer for use in medical diagnostic monitoring and screening, and a method of manufacturing the same are disclosed. A reflectance based, colorimetric test strip reader for use with a mobile device having a jack plug receiving socket, said test strip reader adapted for removably receiving a test strip having a test strip longitudinal axis, comprising a housing; a jack plug operably coupled to and extending from said housing and adapted for operable coupling with said jack plug receiving socket; a test strip adapter including structure defining a test strip receiving channel; a light source oriented within said housing for directing light toward said test strip receiving channel to illuminate a test strip arranged within said test strip adapter; and a light sensor oriented within said housing to sense light reflected from a test strip carried by said test strip receiving channel.

Abstract: A system and method for analysis of colorimetric test strip strips and disease management. The system can include an accessory that is operably coupled to a mobile device, the mobile device acquiring and/or analyzing images of the colorimetric test strips. The light box accessory can be detachably attached to the mobile device, or made to remain attached to the mobile device, but with the capability of having the light box accessory removed from the field of view of the camera for general photography purposes. In other embodiments, an image containing known calibration color(s) and reagent area(s) is obtained sans the light box for comparison with a previous calibration image to model changes in ambient lighting conditions and determine a color correction function. The correction can be applied to the detected reagent area color(s) for matching between the detected reagent area color(s) and reference color(s) on the reference chart.