Abstract: A method is provided for determining a concentration of a target nucleic acid by using a nucleic acid probe labeled with a fluorescent dye. The method comprises: providing, as the probe, a nucleic acid probe capable of reducing fluorescence emission from the fluorescent dye when hybridized with the target nucleic acid; hybridizing the probe to the target nucleic acid; and measuring a decrease in fluorescence emission from the fluorescent dye after the hybridization relative to fluorescence emission from the fluorescent dye before the hybridization.
Type:
Grant
Filed:
January 27, 2006
Date of Patent:
September 2, 2008
Assignees:
Japan Bioindustry Association, Japan as represented by Secretary of Agency of Industrial Science and Technology, KANKYO ENGINEERING Co., Ltd.
Abstract: A method is provided for determining a concentration of a target nucleic acid by using a nucleic acid probe labeled with a fluorescent dye. The method comprises: providing, as the probe, a nucleic acid probe capable of reducing fluorescence emission from the fluorescent dye when hybridized with the target nucleic acid; hybridizing the probe to the target nucleic acid; and measuring a decrease in fluorescence emission from the fluorescent dye after the hybridization relative to fluorescence emission from the fluorescent dye before the hybridization.
Type:
Grant
Filed:
August 1, 2002
Date of Patent:
August 22, 2006
Assignees:
Japan Bioindustry Association, Agency of Industrial Science and Technology, Kankyo Engineering Co., Ltd.
Abstract: A method is provided for determining a concentration of a target nucleic acid by using a nucleic acid probe labeled with a fluorescent dye. The method comprises: providing, as the probe, a nucleic acid probe capable of reducing fluorescence emission from the fluorescent dye when hybridized with the target nucleic acid; hybridizing the probe to the target nucleic acid; and measuring a decrease in fluorescence emission from the fluorescent dye after the hybridization relative to fluorescence emission from the fluorescent dye before the hybridization.
Type:
Application
Filed:
January 27, 2006
Publication date:
August 10, 2006
Applicants:
Japan Bioindustry Association, Japan as represented by Secretary..., KANKYO ENGINEERING Co., Ltd.
Abstract: A method is provided for determining a concentration of a target nucleic acid by using a nucleic acid probe labeled with a fluorescent dye. The method comprises:
providing, as the probe, a nucleic acid probe capable of reducing fluorescence emission from the fluorescent dye when hybridized with the target nucleic acid;
hybridizing the probe to the target nucleic acid; and measuring a decrease in fluorescence emission from the fluorescent dye after the hybridization relative to fluorescence emission from the fluorescent dye before the hybridization.
Type:
Grant
Filed:
November 29, 2000
Date of Patent:
December 17, 2002
Assignees:
Japan Bioindustry Association, Secretary of Agency of Industrial Science and
Technology, Kankyo Engineering Co., Ltd.
Abstract: A method is provided for determining a concentration of a target nucleic acid by using a nucleic acid probe labeled with a fluorescent dye. The method comprises:
providing, as the probe, a nucleic acid probe capable of reducing fluorescence emission from the fluorescent dye when hybridized with the target nucleic acid;
hybridizing the probe to the target nucleic acid; and measuring a decrease in fluorescence emission from the fluorescent dye after the hybridization relative to fluorescence emission from the fluorescent dye before the hybridization.
Type:
Grant
Filed:
November 29, 2000
Date of Patent:
December 10, 2002
Assignees:
Japan Bioindustry Association, Japan as represented by Secretary of Agency of Industrial
Science and Technology, Kankyo Engineering Co., Ltd.
Abstract: The present invention provides a method for isolating a microbe whereby a sample of microbe cells is encapsulated in agarose gel particulates, wherein some of the particulates contain a single cell, and the other particulates contain more than one cell; incubating the particulates in nutritional and environmental conditions that enable the microbe contained in the sample solution that can grow on a plate of a plate culture method to grow in the agarose gel particulate; and isolating the particulates having single cells from the group of the particulates having more than one cell.
Type:
Grant
Filed:
March 3, 2000
Date of Patent:
May 21, 2002
Assignees:
Japan Bioindustry Association, Ajinomoto Co., Inc., Agency of Industrial Science and Technology