Abstract: An object of the present invention is to provide a method for releasing oocyte maturation regulation by suppressing expression of a gene capable of regulating oocyte maturation in shrimps by the RNA interference method. The present invention provides a method for blocking oocyte maturation inhibition in farmed shrimps to be used as spawners (hereafter, “farmed shrimp”), comprising suppressing the expression of a vitellogenesis-inhibiting hormone (VIH) gene in shrimps by RNA interference using double-stranded RNA (dsRNA) targeting mRNA of the vitellogenesis-inhibiting hormone gene in farmed shrimps.

Abstract: An object of the present invention is to provide a method for releasing oocyte maturation regulation by suppressing expression of a gene capable of regulating oocyte maturation in shrimps by the RNA interference method. The present invention provides a method for blocking oocyte maturation inhibition in farmed shrimps to be used as spawners (hereafter, “farmed shrimp”), comprising suppressing the expression of a vitellogenesis-inhibiting hormone (VIH) gene in shrimps by RNA interference using double-stranded RNA (dsRNA) targeting mRNA of the vitellogenesis-inhibiting hormone gene in farmed shrimps.

Abstract: A liquid fuel production method of the present invention includes: a saccharification step in which a biomass is saccharified; a methane fermentation step in which a saccharified liquid acquired in the saccharification step undergoes methane fermentation; and a biogas to liquid (BTL) step in which a liquid fuel is generated from a biogas acquired in the methane fermentation step.

Abstract: The present invention is a cellulose-based biomass juicing method in which a cellulose-based biomass is chipped, and the cellulose-based biomass is juiced after a predetermined pre-treatment is performed on the cellulose-based biomass, wherein the pre-treatment is a mechanical treatment in which parenchyma constituting the cellulose-based biomass is crushed.

Abstract: A method for aligning proteins is provided. The method for aligning the proteins (20 and 32) is capable of aligning proteins (20 and 32) on the substrate (10) comprising carbon. The proteins (20 and 32) are used as structures comprising a cellulose binding domain (22) of the cellulosome and a cohesin domain (21), and a protein (30) to be bonded to the protein (20) is used as a protein (32) having a dockerin domain (31). The cohesin domain (21) can be used as an amino-acid sequence for a cohesin domain (21) derived from various microorganisms, and a functional protein can be used as a protein structure having a microbial-species-specific dockerin domain. The protein (20) can be arrayed regularly along a graphite hexagonal-plate-shaped crystal, and any protein (32) having this dockerin domain (20) can be bonded and arranged as an active protein (32) on the graphite (10).

Abstract: In saccharification of cellulose, chimeric ?-glucosidase having a region exhibiting thermophilic bacteria-derived ?-glucosidase activity and a module combinable to cellulose is used along with cellulosome, and at the completion of saccharification of cellulose, a cellulosic substrate is added to make the chimeric ?-glucosidase and cellulosome attach to the cellulosic substrate for separation.

Abstract: Described herein are methods and materials useful for improving root growth and nutrient uptake in cereal grasses. In particular, present disclosure provides methods for increasing root growth and nutrient uptake in a cereal grass involving marker assisted selection and backcrossing. The present disclosure also provides recombinant DNA for the generation of transgenic plants, transgenic plant cells, and methods of producing the same. The present disclosure also provides materials and methods useful for improving the tolerance of a cereal grass to phosphorus-deficiency The present disclosure further provides methods for generating transgenic seed that can be used to produce a transgenic plant having increased root growth, nutrient uptake, and phosphorus-deficiency tolerance.

Abstract: In saccharification of cellulose, chimeric ?-glucosidase having a region exhibiting thermophilic bacteria-derived ?-glucosidase activity and a module combinable to cellulose is used along with cellulosome, and at the completion of saccharification of cellulose, a cellulosic substrate is added to make the chimeric ?-glucosidase and cellulosome attach to the cellulosic substrate for separation.

Abstract: A method for producing cellulolytic enzyme, where clostridium microorganisms having cellulose utilizability are cultured, wherein after utilizable carbon source is consumed by the clostridium microorganisms, addition and consumption of the carbon source are repeated periodically to accumulate the cellulolytic enzyme in a culture medium.

Abstract: This invention relates to a stress-inducible transcription factor derived from maize, a gene encoding the same, and a method for using the same. Specifically, this invention provides a gene comprising the following DNA (a) or (b): (a) DNA consisting of the nucleotide sequence as shown in SEQ ID NO: 1; or (b) maize-derived DNA hybridizing under stringent conditions with DNA consisting of a nucleotide sequence that is complementary to the DNA consisting of the nucleotide sequence as shown in SEQ ID NO: 1 and encoding a protein that regulates the transcription of a gene located downstream of a stress responsive element. Further, this invention relates to a transgenic plant having improved tolerance to environmental stress, such as high-temperature or dehydration stress, into which such gene has been introduced.

Abstract: A nitrification inhibitor and a soil improver and a fertilizer containing the same are offered which can be utilized in a wide area from a tropical to a temperate zone, and can be easily obtained from natural origin materials. It is a nitrification inhibitor to inhibit soil nitrification, containing as a main component either one of linoleic acid, ?-linolenic acid, ?-linolenic acid, and methyl linoleate, or their mixture of two or more. By incorporating this nitrification inhibitor into a soil improver or a fertilizer, nitrification of soil can be effectively inhibited.

Abstract: A nitrification inhibitor and a soil improver and a fertilizer containing the same are offered which can be utilized in a wide area from a tropical to a temperate zone, and can be easily obtained from natural origin materials. It is a nitrification inhibitor to inhibit soil nitrification, containing juglone as a main component. By incorporating this nitrification inhibitor into a soil improver or a fertilizer, nitrification of soil can be effectively inhibited. The nitrification inhibitor is preferably contained in a soil improver or a fertilizer in the range of 20 to 50% weight %.

Abstract: This invention provides a stress-inducible promoter that effectively functions in monocotyledonous plants such as rice, and environmental stress-tolerant plants using the promoter. Such promoter is derived from rice and consists of the following DNA (a) or (b): (a) DNA that consists of the nucleotide sequence as shown in SEQ ID NO: 1 or 10; or (b) DNA that hybridizes under stringent conditions with DNA consisting of a nucleotide sequence that is complementary to the DNA consisting of the nucleotide sequence as shown in SEQ ID NO: 1 or 10 and that expresses stress-inducible promoter activity. Such environmental stress-tolerant plant has had such promoter introduced therein.

Abstract: The present invention relates to an environmental stress-tolerant plant. The invention discloses a transgenic plant containing a gene in which a DNA encodes a protein consisting of the amino acid sequence as shown in SEQ ID NO: 4 with the deletion of amino acids from positions 136-165 thereof, the DNA being operably linked downstream of a stress responsive promoter.

Abstract: The purpose of the present invention is to determine the nucleotide sequence of the full-length genomic RNA of papaya leaf-distortion mosaic virus. The present invention provides the full-length genomic RNA of papaya leaf-distortion mosaic virus, a method for diagnosing infection with papaya leaf-distortion mosaic virus using the full-length genomic RNA, a method for producing a papaya leaf-distortion mosaic virus-resistant plant, and a method for producing a foreign protein in a plant body.

Abstract: The present invention relates to an environmental stress-tolerant plant. The invention discloses a transgenic plant containing a gene in which a DNA encoding a protein consisting of the amino acid sequence as shown in SEQ ID NO: 4, operably linked downstream of a stress responsive promoter.

Abstract: The present invention relates to an environmental stress-tolerant plant. The invention discloses a transgenic plant containing a gene in which a DNA encoding the following protein (a) or (b) is ligated downstream of a stress responsive promoter: (a) a protein consisting of the amino acid sequence as shown in SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8 or SEQ ID NO: 10; (b) a protein which consists of the amino acid sequence having deletion, substitution or addition of at least one amino acid in the amino acid sequence as shown in SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8 or SEQ ID NO: 10 and which regulates the transcription of genes located downstream of a stress responsive element.

Abstract: According to the present invention, there is provided a transgenic plant transformed with a DNA coding for the Arabidopsis thaliana DREB1A transcription factor of SEQ ID NO: 2, that binds to a stress responsive element, and regulates the transcription of genes located downstream of the element, the transgenic plant having improved tolerance to environmental stresses such as dehydration, low temperature and salt, and being free from dwarfing.

Abstract: According to the present invention, a protein which binds to DRE to activate the transcription of genes located downstream of the DRE, a gene encoding the protein, a recombinant vector comprising the gene, a transformant comprising the recombinant vector, a transgenic plant comprising the gene, and a method for producing the gene using the transformant are provided. The present invention is useful for creating a stress tolerant plant.

Abstract: The present invention discloses a genomic RNA of papaya leaf-distortion mosaic virus (PLDMV) having the base sequence shown in SEQ ID NO: 1 and a DNA complementary to the above RNA having the base sequence shown in SEQ ID NO: 2. These RNA and cDNA are able to render a plant a resisitance to pLDMV and thus extremely useful industrially.