Patents Assigned to JCR Pharmaceuticals Co., Ltd.
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Patent number: 9994641Abstract: Disclosed are a novel anti-human transferrin receptor antibody comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 4, 5, 6 and 7 that can pass through the blood-brain barrier, a fusion protein comprising a protein necessary to be brought into function in the central nervous system and the antibody, and method of their production. The fusion protein comprises amino acid sequences of an anti-human transferrin receptor antibody that recognizes an amino acid sequence selected from the group consisting of SEQ ID NOs:1, 2, and 3, and of other protein that is bound thereto on the C-terminal side.Type: GrantFiled: December 24, 2014Date of Patent: June 12, 2018Assignee: JCR PHARMACEUTICALS CO., LTD.Inventors: Hiroyuki Sonoda, Hideto Morimoto, Yuri Koshimura, Masafumi Kinoshita, Haruna Takagi, Yoshiko Yoshii
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Patent number: 9896715Abstract: Disclosed is a method which enables semiquantitative or quantitative determination of the ratio between cysteine and formylglycine residues in a protein. The method includes (a) a step of labeling the protein (i) with a halogen-substituted carboxylic acid, (ii) with a halogen-substituted carboxylic acid amide, and (iii) with a halogen-substituted carboxylic acid and then with hydrazine, or with a halogen-substituted carboxylic acid and then by oximation, (b) a step of digesting each labeled protein to provide a corresponding mixture of peptide fragments, (c) a step of subjecting each mixture to reverse phase chromatography to separate the peptide fragments from each other to produce a chromatogram, (d) a step of comparing the produced chromatograms with each other to identify the peak corresponding to the peptide fragment that contained a cysteine residue and the peak corresponding to the peptide fragment that contained a formylglycine residue.Type: GrantFiled: December 18, 2012Date of Patent: February 20, 2018Assignee: JCR PHARMACEUTICALS CO., LTD.Inventor: Tetsuo Yokoyama
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Patent number: 9744310Abstract: The pharmaceutical injection device includes a pharmaceutical syringe mounting portion (3) provided within a main body case (2) and to which a pharmaceutical syringe (4) is removably mounted, a piston (5) provided movably with respect to the pharmaceutical syringe (4) mounted to the pharmaceutical syringe mounting portion (3), a drive mechanism (6) for driving the piston (5), a controller (7) that is electrically connected to the drive mechanism (6), a display section (35) that is connected to the controller (7), and an encoder (26) that is connected to the controller (7) and detects the amount of the pharmaceutical remaining inside the pharmaceutical syringe (4). The controller (7) displays a warning on the display section (35) recommending removal of the pharmaceutical syringe (4) from the pharmaceutical syringe mounting portion (3) when the presence of a pharmaceutical is detected by the encoder (26).Type: GrantFiled: September 10, 2012Date of Patent: August 29, 2017Assignees: PANASONIC HEALTHCARE CO., LTD., JCR PHARMACEUTICALS CO., LTD.Inventors: Tsuguhiro Kondoh, Seiji Kikuchi, Takashi Hanada
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Publication number: 20170233704Abstract: Disclosed is a method for production of recombinant human DNase I which is of such high purity as may be directly used as a medical drug. The method includes the steps of; culturing recombinant DNase I-producing mammalian cells, subjecting a culture supernatant to an anion-exchange column chromatography, subjecting to a column chromatography employing as solid phase a material having affinity for phosphate group, subjecting to a cation-exchange column chromatography, and subjecting to a dye affinity column chromatography.Type: ApplicationFiled: April 28, 2017Publication date: August 17, 2017Applicant: JCR PHARMACEUTICALS CO., LTD.Inventors: Yuri KOSHIMURA, Miroslav MATEV, Hiroyuki SONODA
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Patent number: 9657310Abstract: Disclosed are a novel expression vector for efficient expression of recombinant proteins in mammalian cells, a mammalian cell transformed with the vector, and a method for production of the mammalian cell. The expression vector is an expression vector for expression of a mammalian protein and includes a gene expression regulatory site, and a gene encoding the protein downstream thereof, and an internal ribosome entry site further downstream thereof, and a gene encoding a glutamine synthetase further downstream thereof, and a dihydrofolate reductase gene downstream of either the same gene expression regulatory site or another gene expression regulatory site in addition to the former.Type: GrantFiled: April 25, 2013Date of Patent: May 23, 2017Assignee: JCR PHARMACEUTICALS CO., LTD.Inventors: Kenichi Takahashi, Shinji Kakimoto
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Patent number: 9623180Abstract: This pharmaceutical injection system is provided with a pharmaceutical injection device, a charging device, a detector, and an alarm section. The pharmaceutical injection device has a main body case and a pharmaceutical syringe mounting portion provided within the main body case and to which a pharmaceutical syringe is removably mounted. The pharmaceutical injection device is placed in the charging device, and the charging device has a charger for charging the pharmaceutical injection device. The detector is provided to the pharmaceutical injection device and/or the charging device and detects whether or not the pharmaceutical syringe has been mounted to the pharmaceutical syringe mounting portion. The alarm section is connected to the detector and emits an alarm when it is detected that the pharmaceutical syringe has been mounted onto the pharmaceutical syringe mounting portion.Type: GrantFiled: September 10, 2012Date of Patent: April 18, 2017Assignees: PANASONIC HEALTHCARE CO., LTD., JCR PHARMACEUTICALS CO., LTD.Inventors: Toshiaki Iio, Yukihiro Takabatake, Seiji Kikuchi, Tsuguhiro Kondoh, Shinsuke Hata, Takashi Hanada
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Patent number: 9617529Abstract: Disclosed is a method for production of recombinant human alpha-galactosidase A (rh alpha-Gal A) in a large scale, with a high purity. The method comprises the steps of (a) culturing rh alpha-Gal A-producing mammalian cells in a serum-free medium, (b) collecting culture supernatant, (c) subjecting the culture supernatant to anion-exchange column chromatography, (d) to hydrophobic column chromatography, (e) to a column chromatography employing as solid phase a material having affinity for phosphate group, (f) to cation-exchange column chromatography, and (g) to gel filtration column chromatography, in the order.Type: GrantFiled: July 24, 2013Date of Patent: April 11, 2017Assignee: JCR PHARMACEUTICALS CO., LTD.Inventors: Masahiro Asano, Toshihiro Yagi, Tsuyoshi Fukui, Atsuko Kawasaki, Yukichi Hatano, Kazutoshi Mihara, Atsushi Sugimura
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Publication number: 20170073633Abstract: Provided are a novel medium for expressing glycoproteins by culturing cells and a method for producing glycoproteins by culturing cells in said medium. Further provided are a medium comprising uridine and N-acethyl-D-mannosamine for the use of expression of a glycoprotein by culturing cells and a method for producing glycoproteins by culturing cells in said medium.Type: ApplicationFiled: November 30, 2016Publication date: March 16, 2017Applicant: JCR Pharmaceuticals Co., Ltd.Inventors: Miroslav MATEV, Kenichi Takahashi, Shinji Kakimoto, Ayaka Kotani
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Patent number: 9526831Abstract: This pharmaceutical injection device comprises a main body case (2) that has an injection needle insertion and retraction opening (1); pharmaceutical syringe mounting portion (3) that is provided within the main body case (2); a piston (5) that is provided movably with respect to a pharmaceutical syringe (4) that is mounted to the pharmaceutical syringe mounting portion (3); a drive mechanism (6) that drives the piston (5); a controller (7) that is electrically connected to the drive mechanism (6); and a display section (35) that is electrically connected to the controller (7). The controller (7) updates the display content that is displayed on the display section (35) according to the number of times the drive mechanism (6) has been driven, and saves this updated display content in a memory (51), so that when the drive mechanism (6) has been driven a specific number of times, the display contents during the various instances of drive that were saved in the memory (51) are combined.Type: GrantFiled: September 10, 2012Date of Patent: December 27, 2016Assignees: PANASONIC HEALTHCARE CO., LTD., JCR PHARMACEUTICALS CO., LTD.Inventors: Tsuguhiro Kondoh, Seiji Kikuchi, Takashi Hanada
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Patent number: 9279109Abstract: Disclosed is a method for production of recombinant human iduronate 2-sulfatase (rhI2S) (the 26th to 550th amino acids of SEQ ID NO: 10) in a large scale, with a high purity, and mannose 6-phosphate residues. The method comprises the steps of (a) culturing rhI2S (the 26th to 550th amino acids of SEQ ID NO: 10)-producing mammalian cells in a serum-free medium, (b) collecting culture supernatant, (c) subjecting the culture supernatant to cation-exchange column chromatography, (d) to dye affinity column chromatography, (e) to anion-exchange column chromatography, and (f) to a column chromatography employing as solid phase a material having affinity for phosphate group, and (g) to gel filtration column chromatography, in the order.Type: GrantFiled: January 23, 2012Date of Patent: March 8, 2016Assignee: JCR Pharmaceuticals Co., Ltd.Inventors: Kazutoshi Mihara, Atsuko Kawasaki, Kouta Ootsuki, Yuukichi Hatano, Shoichiro Kamei, Atsushi Sugimura
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Publication number: 20160008408Abstract: [Problem] To provide a method for producing human corneal epithelial sheet, wherein the human corneal epithelial-derived cells obtained by culturing human corneal epithelial cells are cultured on an amnion substrate. [Solution] A method for culturing human corneal epithelial cells using mesenchymal stem cells as the feeder cells; and a method for culturing human corneal epithelial cells using a medium containing a ROCK inhibitor, a phosphodiesterase inhibitor, a MAP kinase inhibitor and a TGF-? receptor inhibitor in various combinations.Type: ApplicationFiled: March 9, 2014Publication date: January 14, 2016Applicants: JCR PHARMACEUTICALS CO., LTD., KYOTO PREFECTURAL PUBLIC UNIVERSITY CORPORATIONInventors: Kiwamu IMAGAWA, Kenichi MAEDA, Yuki HOSODA, Shuichi YOKOYAMA, Naoki OKUMURA, Noriko KOIZUMI, Shigeru KINOSHITA
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Publication number: 20140228766Abstract: This pharmaceutical injection system is provided with a pharmaceutical injection device, a charging device, a detector, and an alarm section. The pharmaceutical injection device has a main body case and a pharmaceutical syringe mounting portion provided within the main body case and to which a pharmaceutical syringe is removably mounted. The pharmaceutical injection device is placed in the charging device, and the charging device has a charger for charging the pharmaceutical injection device. The detector is provided to the pharmaceutical injection device and/or the charging device and detects whether or not the pharmaceutical syringe has been mounted to the pharmaceutical syringe mounting portion. The alarm section is connected to the detector and emits an alarm when it is detected that the pharmaceutical syringe has been mounted onto the pharmaceutical syringe mounting portion.Type: ApplicationFiled: September 10, 2012Publication date: August 14, 2014Applicants: JCR PHARMACEUTICALS CO., LTD., PANASONIC HEALTHCARE CO., LTD.Inventors: Toshiaki Iio, Yukihiro Takabatake, Seiji Kikuchi, Tsuguhiro Kondoh, Shinsuke Hata, Takashi Hanada
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Publication number: 20140228763Abstract: This pharmaceutical injection device comprises a main body case (2) that has an injection needle insertion and retraction opening (1); pharmaceutical syringe mounting portion (3) that is provided within the main body case (2); a piston (5) that is provided movably with respect to a pharmaceutical syringe (4) that is mounted to the pharmaceutical syringe mounting portion (3); a drive mechanism (6) that drives the piston (5); a controller (7) that is electrically connected to the drive mechanism (6); and a display section (35) that is electrically connected to the controller (7). The controller (7) updates the display content that is displayed on the display section (35) according to the number of times the drive mechanism (6) has been driven, and saves this updated display content in a memory (51), so that when the drive mechanism (6) has been driven a specific number of times, the display contents during the various instances of drive that were saved in the memory (51) are combined.Type: ApplicationFiled: September 10, 2012Publication date: August 14, 2014Applicants: JCR PHARMACEUTICALS CO., LTD., PANASONIC HEALTHCARE CO., LTD.Inventors: Tsuguhiro Kondoh, Seiji Kikuchi, Takashi Hanada
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Publication number: 20140221925Abstract: The pharmaceutical injection device includes a pharmaceutical syringe mounting portion (3) provided within a main body case (2) and to which a pharmaceutical syringe (4) is removably mounted, a piston (5) provided movably with respect to the pharmaceutical syringe (4) mounted to the pharmaceutical syringe mounting portion (3), a drive mechanism (6) for driving the piston (5), a controller (7) that is electrically connected to the drive mechanism (6), a display section (35) that is connected to the controller (7), and an encoder (26) that is connected to the controller (7) and detects the amount of the pharmaceutical remaining inside the pharmaceutical syringe (4). The controller (7) displays a warning on the display section (35) recommending removal of the pharmaceutical syringe (4) from the pharmaceutical syringe mounting portion (3) when the presence of a pharmaceutical is detected by the encoder (26).Type: ApplicationFiled: September 10, 2012Publication date: August 7, 2014Applicants: JCR PHARMACEUTICALS CO., LTD., PANASONIC HEALTHCARE CO., LTD.Inventors: Tsuguhiro Kondoh, Seiji Kikuchi, Takashi Hanada
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Patent number: 8759288Abstract: Means for improving the success rate of pregnancy on the basis of blastocyst transfer is disclosed. The means comprises an agent for promoting pregnancy in blastocyst transfer comprising the supernatant of the culture which is obtained by culturing a human embryo in a medium until the embryo develops into a blastocyst. Also disclosed are a method for production of the agent, as well as a method for promoting pregnancy comprising; culturing a human embryo in a medium until the human embryo develops into a blastocyst, injecting a composition comprising the supernatant of the culture into the uterine cavity of a patient who is to undergo blastocyst transfer, and then transferring the blastocyst to the recipient.Type: GrantFiled: September 25, 2012Date of Patent: June 24, 2014Assignee: JCR Pharmaceuticals Co., Ltd.Inventors: Masahide Shiotani, Sakae Goto
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Patent number: 8710033Abstract: The objective is to find compounds which have an activity to improve the success rate of pregnancy and implantation in blastocyst transfer in mammals, to provide a method of producing and using the compounds, and to provide a pregnancy-promoting agent. Disclosed is a pregnancy-promoting agent containing one or more lysophosphatidic acids selected from the group consisting of LPA-C16:0, LPA-C16:1, LPA-C18:0, LPA-C18:1 and LPA-C18:2.Type: GrantFiled: August 25, 2010Date of Patent: April 29, 2014Assignees: JCR Pharmaceuticals Co., Ltd.Inventors: Masahide Shiotani, Sakae Goto, Mitsuo Shimizu
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Patent number: 8685691Abstract: Disclosed is an efficient method for production of aminopeptidase. The method comprises either transforming host bacteria with an aminopeptidase gene and with a neutral protease gene, or transforming some part of host bacteria with an aminopeptidase gene while transforming the other part of the host bacteria with a neutral protease gene, culturing in a medium the hose bacteria transformed with the aminopeptidase gene and with the neutral protease gene, or culturing a mixture of the host bacteria transformed with the aminopeptidase gene and the host bacteria transformed with the neutral protease gene, to let both the aminopeptidase and the neutral protease be expressed, and collecting the aminopeptidase thus produced from the culture mixture.Type: GrantFiled: October 25, 2008Date of Patent: April 1, 2014Assignee: JCR Pharmaceuticals Co., Ltd.Inventors: Hiroyuki Sonoda, Katsuya Daimon, Atsushi Sugimura
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Patent number: 8673648Abstract: Disclosed is a method separation analysis of reducing sugars with enhanced sensitivity and an analytical apparatus therefore employing the post-column fluorescence detection/boric acid complex anion exchange method. The method disclosed is a method for analysis of reducing sugars using the post-column fluorescence detection/boric acid complex anion exchange method, and characterized in that a back-pressure generator is installed in the flow path between the heater, which is for causing a reaction by heating a sample separated by column chromatography with a basic amino acid, and a fluorometric detector.Type: GrantFiled: April 25, 2011Date of Patent: March 18, 2014Assignee: JCR Pharmaceuticals Co., Ltd.Inventor: Tetsuo Yokoyama
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Publication number: 20140051175Abstract: Disclosed are an apparatus and method for separation analysis of mannose-6-phosphate (M6P) by post-column fluorescence detection method. The apparatus is based on chromatography, and includes a column with a solid phase having affinity for phosphate, a flow path for the eluate, a heater installed on the flow path for M6P and a basic amino acid to react by heating the eluate in the flow path, and a fluorescence detector installed downstream of the heater for continuously irradiating the eluate with excitation light and measuring the intensity of the emission, and may include in the flow path a supply channel for addition of a basic amino acid between the column and the heater. The method is characterized in that it uses the apparatus and a second mobile phase consisting of a second buffer containing phosphate of predetermined concentration and adjusted to a predetermined pH.Type: ApplicationFiled: March 26, 2012Publication date: February 20, 2014Applicant: JCR PHARMACEUTICALS CO., LTD.Inventor: Tetsuo Yokoyama
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Patent number: 8647613Abstract: The present invention has an object of providing a drug carrier capable of controlling in vivo pharmacokinetics. The present invention is directed to a drug carrier comprising a molecular assembly having a drug incorporated therein, and the above object can be achieved by a part of the amphiphilic molecules included in the molecular assembly being released from the molecular assembly by an external environmental change. The present invention utilizes a phenomenon that the hydrophilic-hydrophobic balance of the amphiphilic molecules is shifted toward hydrophilicity by an external environmental change and thus the amphiphilic molecules are freed from the molecular assembly.Type: GrantFiled: March 10, 2006Date of Patent: February 11, 2014Assignees: JCR Pharmaceuticals Co., Ltd., Waseda UniversityInventors: Shinji Takeoka, Yosuke Okamura, Hideo Kanazawa, Shuji Hisamoto, Kohei Kubota, Yosuke Obata