Abstract: A liquid chromatography column, utilizing horizontal or radial flow of sample material passing there through, includes a housing defining a chamber therein, at least one removable screw lid, and first and second longitudinally extending porous frits positioned within the chamber. A bed or packing of particulate, chromatographic separation material is positioned within the chamber and intermediate the porous frits, the first of the porous frits being adjacent the housing and an inlet channel, the second of the porous frits being positioned adjacent a core member and an outlet channel. A distributor is operatively connected to the inlet channel, and a collector is connected to the outlet channel. The distributor and the inlet channel are constructed to direct associated material to be separated in the bed evenly across a longitudinal length of the bed in a horizontal direction.

Abstract: A chromatography column to bind molecules or proteins, is filled with a replaceable matrix and is provided with fill elements with which a single feed tube can be connected to an across the column distributed fill element. The fill element keeps an inner land area free from fill openings which can be closed. With the fill element it is possible to dispense the matrix into the column in an equally spread manner. Elements are present to internally flush the feed tube, while there are features to make the flushing impossible during filling with matrix.