Abstract: Disclosed is a method of purifying a botulinum toxin including (a) pre-treating a culture solution containing a botulinum toxin, (b) purifying the pre-treated botulinum toxin using anion exchange chromatography and (c) purifying the botulinum toxin using cation exchange chromatography. The method is capable of purifying a botulinum toxin with high purity and activity using a simple process including anion exchange chromatography and cation exchange chromatography, and is thus useful for botulinum toxin production.

Abstract: Disclosed is a method of purifying a botulinum toxin comprising (a) pre-treating a culture solution containing a botulinum toxin, (b) purifying the pre-treated botulinum toxin using cation exchange chromatography, (c) purifying the botulinum toxin using hydrophobic chromatography, and (d) purifying the botulinum toxin using anion exchange chromatography. The method is capable of purifying a botulinum toxin with high purity and activity and is thus useful for botulinum toxin production.

Abstract: The present invention relates to a method for purifying botulinum toxin (BTX). More specifically, the method is performed in the order of purification steps using cation exchange chromatography (CEX), hydrophobic interaction chromatography (HIC), and mixed mode chromatography (cation exchange (phosphate) and affinity (calcium)), the method further comprising the step of treating trypsin to activate the botulinum toxin. Therefore, the method can purify botulinum toxin having excellent purity and activity and thus can be useful in producing botulinum toxin.

Abstract: Disclosed are a medium composition for culturing Clostridium botulinum comprising a potato peptone, a yeast extract and glucose, and a method of preparing a botulinum toxin using the same. The medium excludes animal-derived products and major allergens, and enables the botulinum toxin-producing strain to reach the maximum growth level within a short time, thereby providing effects of shortening the production time and reducing the production cost in the botulinum toxin production process.

Abstract: The present invention relates to a method of culturing a strain producing botulinum toxin for increasing botulinum toxin productivity, and a method of effectively producing botulinum toxin by culturing the strain by the method. The culture method of the present invention has the advantage of enabling commercial productivity by increasing botulinum toxin productivity 10-fold or more.

Abstract: Disclosed are a medium composition for culturing Clostridium botulinum comprising a potato peptone, a yeast extract and glucose, and a method of preparing a botulinum toxin using the same. The medium excludes animal-derived products and major allergens, and enables the botulinum toxin-producing strain to reach the maximum growth level within a short time, thereby providing effects of shortening the production time and reducing the production cost in the botulinum toxin production process.

Abstract: The present invention relates to a suturing thread for providing a more efficient lift procedure, and provides a suturing thread for a facelift and a body lift, wherein the suturing thread is made of a polymer material and has a plurality of conical protrusions or a plurality of funneled protrusions formed on an outer circumferential surface of a main thread of the suturing thread.

Abstract: The present invention relates to a suturing thread for providing a more efficient lift procedure, and provides a suturing thread for a facelift and a body lift, wherein the suturing thread is made of a polymer material and has a plurality of conical protrusions or a plurality of funneled protrusions formed on an outer circumferential surface of a main thread of the suturing thread.