Abstract: A viral vector delivery system for both respiratory and digestive tracts of pigs and an application thereof are provided. The viral vector delivery system includes a backbone plasmid and a helper plasmid. The backbone plasmid is produced by inserting a full-length cDNA of porcine enterovirus B (PEVB) into a pUC57 plasmid. The helper plasmid is produced by inserting a green fluorescent protein-coding gene into a plasmid pCAG-T7-polymerase. The viral vector delivery system can be constructed with high efficiency, can quickly cause a cytopathic effect (CPE) after infecting cells, has a viral titer up to 107.75 TCID50/mL, and can maintain high stability. A pathogenic epitope for the respiratory and digestive tracts of pigs is inserted into the backbone plasmid to produce a vaccine antigen, which is non-pathogenic, has high stability and a high viral titer, and allows a prominent immunization effect after being inoculated in pigs.

Abstract: An application of an OBF1 transcription factor in chromosome doubling of a plant and a transgenic plant cultivation method are provided. The gene sequence of the OBF1 transcription factor is shown in SEQ ID NO: 1; and the plant includes petunias, tomatoes, and tobaccoes. An OBF1 gene is introduced into plant cells or tissues, and under treatment with kanamycin, a chromosome-doubled transgenic cell line or plant is obtained; and the transgenic plant grows vigorously and exhibits resistance to biotic stress. After treatment according to the present invention, plants still easily self-pollinate, thus enabling efficient separation of doubled offspring plants, and the doubling trait can be stably inherited.

Abstract: Use of a gene ZmNPF6.8 in regulation of a nitrogen uptake and utilization efficiency of Zea mays L. and a yield per Zea mays L. plant is provided. The gene ZmNPF6.8 has: 1) a coding region nucleotide sequence shown in SEQ ID NO: 3; or 2) a nucleotide sequence that has 90% or more homology with the coding region nucleotide sequence shown in SEQ ID NO: 3 and encodes a same functional protein as the coding region nucleotide sequence shown in SEQ ID NO: 3. In the present disclosure, a Mutator transposon can be inserted into an amino acid-coding region of the gene ZmNPF6.8 to down-regulate an expression level of the gene and significantly reduce a nitrogen uptake and utilization efficiency of Zea mays L. and a yield per Zea mays L. plant.

Abstract: Soyasaponin-related Kompetitive Allele Specific polymerase chain reaction (KASP) markers and an application thereof are provided in the present application, belonging to the technical field of molecular breeding. The KASP markers include one or two of S07_43139773G/T, and S07_43139033A/G; the S07_43139773G/T comprises a base of G/T at a position of 43139773 bp on chromosome 7 of a soybean genome, the S07_43139033A/G comprises a base of A/G at a position of 43139033 bp on the chromosome 7 of the soybean genome. The KASP markers are applied to improve soyasaponin germplasm breeding.

Abstract: A method for breeding an improved variety of a forage sorghum-Bacillus megaterium symbiont, and a CAPS marker for rapidly identifying Bacillus megaterium BM18-2. The method includes: when a stigma of a female parent is exerted for 2-3 d, spraying a Bacillus megaterium solution to the stigma, then pollinating same with pollens from anthers of a male parent after the anthers are exerted for 2-5 d, and harvesting mature seeds. The CAPS marker mainly includes a primer pair designed for an SNP site and endonuclease BspT104I, and upstream and downstream primers are respectively SEQ ID Nos: 1-2. The first method for breeding improved variety of forage sorghum containing endophytic Bacillus megaterium. Produced seeds can significantly increase biomass, crude protein, soluble sugar content and roughage grading index (GI) of plants grown in soil with a salt content ?8‰; and the CAPS marker can realize rapid qualitative identification of Bacillus megaterium BM18-2.

Abstract: A method for cultivating alcohol-brewing sorghum on the basis of a biodegradable mulching film includes five steps such as farmland preparation, film mulching, sowing operation, field management and harvesting. Compared with a traditional alcohol-brewing sorghum planting method, the method of the present invention can effectively bring forward the planting time of alcohol-brewing sorghum, reduce the sowing amount of alcohol-brewing sorghum, assist in increasing the germination rate of alcohol-brewing sorghum, and promote the growth and development of alcohol-brewing sorghum; moreover, the method of the present invention effectively reduces the labor intensity and cost of a weeding operation in field management.

Abstract: A molecular marker significantly associated with vitamin E content in soybeans, a Kompetitive Allele-Specific polymerase chain reaction (KASP) primers combination and an application thereof are provided in the present disclosure, belonging to the field of molecular genetics and breeding. A nucleotide sequence of the molecular marker is shown in SEQ ID NO. 1, and there is an A/T mutation at the 21st base. The KASP primer set for detecting the molecular marker includes an upstream primer F1 with a nucleotide sequence as shown in SEQ ID NO. 2, an upstream primer F2 with a nucleotide sequence as shown in SEQ ID NO. 3 and a downstream primer r with a nucleotide sequence as shown in SEQ ID NO. 4. The KASP primers combination developed by the present disclosure accurately distinguish soybeans with high and low vitamin E content.

Abstract: A composition and a kit for detecting mycoplasma are provided. The composition for detecting mycoplasma is an aqueous solution including a primer M-F, a primer M-R, and a probe M-P. A sequence of the M-F is shown in SEQ ID NO: 1. A sequence of the M-R is shown in SEQ ID NO: 2. A nucleotide sequence of the probe M-P is shown in SEQ ID NO: 3, and includes a fluorophore FAM linked at a 5? terminus and a quencher BHQ1 linked at a 3? terminus. The composition exhibits high sensitivity, strong specificity, and a wide detection range when used in the detection of mycoplasma.

Abstract: A multiplex KASP marker primer set for a set of wheat plant height major genes, consisting of a pre-primer having a nucleotide sequence as shown in SEQ ID NO. 10, a post-primer having a nucleotide sequence as shown in SEQ ID NO. 11, and a universal primer having a nucleotide sequence as shown in SEQ ID NO. 9. The multiplex KASP marker primer set achieves the simultaneous detection of the Rht-B1 and Rht-D1 genes.

Abstract: An Exiguobacterium indicum YAN2 having high-concentration selenite tolerance, the preservation number thereof being GDMCC No. 61594, and a method for fermenting inorganic selenium into nano-selenium by using the strain are provided. The obtained nano-selenium can promote the growth of leaf vegetables, increase the content of chlorophyll, Vc, and total phenol, etc, reduce the content of nitrate, and improve the quality of leaf vegetables. In addition, the present application further provides a nano-selenium liquid fertilizer prepared by fermentation using the strain.

Abstract: The present invention provides a preparation method for a modified cathode of an MEC-AD system and application of the system with high efficiency and low energy consumption. A NiCo-BDC hydrothermal carbon composite MOF material is designed and used for modifying a cathode, which can remove COD more rapidly compared with traditional AD. A wind energy and light energy complementary power generation device is used for energy supply of the MEC-AD system, and energy consumption is further reduced by setting an intermittent power supply mode. The intermittent power supply achieves higher energy recovery efficiency while obtaining higher performance, can effectively reduce energy consumption of the MEC-AD system, is conducive to more convenient configuration of a power generation and energy supply device using clean energy, and has a good application prospect in reducing COD in organic wastewater with low energy consumption and high efficiency.

Abstract: Provided is a multi-layer, intelligent and environment-friendly breeding structure for meat waterfowl, including house bodies, a manure treatment room, staircases and feeders. A bottom end net bed and an upper layer net bed are fixedly connected in the house body, and the upper layer net bed is located above the bottom end net bed. Manure dropping mechanisms are arranged side by side between the bottom end net bed and the upper layer net bed. Conveying mechanisms are arranged respectively on both sides of each of the manure dropping mechanisms. A plurality of conveyor belt conveyors are arranged side by side below the bottom end net bed. A transfer mechanism is arranged at one side of the each of the house bodies, and the transfer mechanism is used for collecting manure on the conveyor belt conveyors and the conveying mechanisms and transporting the manure to the fermentation mechanism.

Abstract: An Exiguobacterium indicum YAN2 having high-concentration selenite tolerance, the preservation number thereof being GDMCC No. 61594, and a method for fermenting inorganic selenium into nano-selenium by using the strain are provided. The obtained nano-selenium can promote the growth of leaf vegetables, increase the content of chlorophyll, Vc, and total phenol, etc, reduce the content of nitrate, and improve the quality of leaf vegetables. In addition, the present application further provides a nano-selenium liquid fertilizer prepared by fermentation using the strain.

Abstract: The present disclosure relates to a method for efficiently enriching lutein in broccoli sprouts under ?-aminobutyric acid combined with sodium chloride stress. In the present disclosure, Qingfeng broccoli seeds with plump particles, uniform size and germination ability are selected as raw materials; after removing impurities and being disinfected by a NaClO solution, the seeds are sprayed with distilled water for one day, then sprayed with a mixed aqueous solution of NaCl and ?-aminobutyric acid, to obtain broccoli sprouts.

Abstract: Disclosed are a method for preparing a porcine-derived interferon-delta 5 (pIFN-?5) and an application of the pIFN-?5, where the method for preparing pIFN-?5 includes the following steps: step S1, obtaining a DNA fragment containing pIFN-?5 gene through reverse transcription-polymerase chain reaction (RT-PCR) amplification by using the total RNA of pretreated porcine small intestinal epithelial cells IPEC-J2; step S2, inserting the DNA fragment containing pIFN-?5 gene into an exogenous expression vector to construct a recombinant expression vector for expressing the pIFN-?5 gene; and step S3, introducing the recombinant expression vector into a suitable host cell, and driving the host cell to express the pIFN-?5 gene to obtain the pIFN-?5. The recombinant pIFN-?5 protein is used to prepare drugs or preparations for inhibiting infection of porcine epidemic diarrhea virus (PEDV), porcine transmissible gastroenteritis virus (TGEV), porcine delta coronavirus (PDCoV) and porcine rotavirus (PRoV).

Abstract: A Streptococcus suis (S. suis) vaccine is provided. For the S. suis vaccine, an antigen is a protein with an amino acid sequence shown in SEQ ID NO: 2. A preparation method of the S. suis vaccine is provided, including the following steps: mixing a white oil and aluminum stearate to obtain a white oil adjuvant; adding poly sorbate 80 to an aqueous solution of the protein with the amino acid sequence shown in SEQ ID NO: 2, and thoroughly mixing to obtain an antigen solution; and mixing the antigen solution with the white oil adjuvant according to a volume ratio of (0.5-1.5):2, and emulsifying to obtain the S. suis vaccine. An animal immunized with the S. suis vaccine of the present disclosure can effectively resist the attack of S. suis serotype 2, 3, and 31, with a vaccine protection rate as high as 100%.

Abstract: A preparation method of a crop straw-based seawater desalinator and a product thereof, and the preparation process is as follows: (1) crushing crop straws, and screening the crushed crop straws through a mesh sieve to obtain straw powder; (2) calcining the straw powder at a high temperature under nitrogen protection to obtain biochar, and then putting the biochar into a ball mill for ball milling to obtain ball-milled biochar; (3) adding the straw powder into a sodium hydroxide solution for reaction, and centrifuging a reactant to obtain a viscous product; and (4) stirring the viscous product uniformly with the ball-milled biochar to obtain a mixture, pouring the mixture into a mold, and freezing and drying the mixture to obtain a seawater desalinator. The evaporator features high evaporation rate and the photothermal conversion efficiency, and the ion concentration of the water body after evaporation is greatly reduced.

Abstract: A preparation method of a crop straw-based seawater desalinator and a product thereof, and the preparation process is as follows: (1) crushing crop straws, and screening the crushed crop straws through a mesh sieve to obtain straw powder; (2) calcining the straw powder at a high temperature under nitrogen protection to obtain biochar, and then putting the biochar into a ball mill for ball milling to obtain ball-milled biochar; (3) adding the straw powder into a sodium hydroxide solution for reaction, and centrifuging a reactant to obtain a viscous product; and (4) stirring the viscous product uniformly with the ball-milled biochar to obtain a mixture, pouring the mixture into a mold, and freezing and drying the mixture to obtain a seawater desalinator. The evaporator features high evaporation rate and the photothermal conversion efficiency, and the ion concentration of the water body after evaporation is greatly reduced.

Abstract: A Streptococcus suis (S. suis) vaccine is provided. For the S. suis vaccine, an antigen is a protein with an amino acid sequence shown in SEQ ID NO: 2. A preparation method of the S. suis vaccine is provided, including the following steps: mixing a white oil and aluminum stearate to obtain a white oil adjuvant; adding poly sorbate 80 to an aqueous solution of the protein with the amino acid sequence shown in SEQ ID NO: 2, and thoroughly mixing to obtain an antigen solution; and mixing the antigen solution with the white oil adjuvant according to a volume ratio of (0.5-1.5):2, and emulsifying to obtain the S. suis vaccine. An animal immunized with the S. suis vaccine of the present disclosure can effectively resist the attack of S. suis serotype 2, 3, and 31, with a vaccine protection rate as high as 100%.

Abstract: A method for creating a new germplasm of a male sterile crop by gene editing and an application thereof are provided. In this method, the gene editing is performed on an exon region of a Ty-5 gene, and a deletion of DNA sequence is introduced by using a repair mechanism of plants themselves to double-strand breaks (DSBs), causing a loss of function of Ty-5 gene, thereby obtaining a male-sterile character. The method can be applied without being limited by crop categories. After the gene editing is performed on Ty-5 genes of various crops, new germplasms can be quickly obtained. The new germplasms have the same agronomic characters as the previous materials, and only differ in sexual aspect, which effectively solves the problem of the lack of male sterile materials and unstable fertility in natural resources.