Abstract: Disclosed are a method for preparing a porcine-derived interferon-delta 5 (pIFN-?5) and an application of the pIFN-?5, where the method for preparing pIFN-?5 includes the following steps: step S1, obtaining a DNA fragment containing pIFN-?5 gene through reverse transcription-polymerase chain reaction (RT-PCR) amplification by using the total RNA of pretreated porcine small intestinal epithelial cells IPEC-J2; step S2, inserting the DNA fragment containing pIFN-?5 gene into an exogenous expression vector to construct a recombinant expression vector for expressing the pIFN-?5 gene; and step S3, introducing the recombinant expression vector into a suitable host cell, and driving the host cell to express the pIFN-?5 gene to obtain the pIFN-?5. The recombinant pIFN-?5 protein is used to prepare drugs or preparations for inhibiting infection of porcine epidemic diarrhea virus (PEDV), porcine transmissible gastroenteritis virus (TGEV), porcine delta coronavirus (PDCoV) and porcine rotavirus (PRoV).

Abstract: The present disclosure relates to a method for efficiently enriching lutein in broccoli sprouts under ?-aminobutyric acid combined with sodium chloride stress. In the present disclosure, Qingfeng broccoli seeds with plump particles, uniform size and germination ability are selected as raw materials; after removing impurities and being disinfected by a NaClO solution, the seeds are sprayed with distilled water for one day, then sprayed with a mixed aqueous solution of NaCl and ?-aminobutyric acid, to obtain broccoli sprouts.

Abstract: A Streptococcus suis (S. suis) vaccine is provided. For the S. suis vaccine, an antigen is a protein with an amino acid sequence shown in SEQ ID NO: 2. A preparation method of the S. suis vaccine is provided, including the following steps: mixing a white oil and aluminum stearate to obtain a white oil adjuvant; adding poly sorbate 80 to an aqueous solution of the protein with the amino acid sequence shown in SEQ ID NO: 2, and thoroughly mixing to obtain an antigen solution; and mixing the antigen solution with the white oil adjuvant according to a volume ratio of (0.5-1.5):2, and emulsifying to obtain the S. suis vaccine. An animal immunized with the S. suis vaccine of the present disclosure can effectively resist the attack of S. suis serotype 2, 3, and 31, with a vaccine protection rate as high as 100%.

Abstract: A preparation method of a crop straw-based seawater desalinator and a product thereof, and the preparation process is as follows: (1) crushing crop straws, and screening the crushed crop straws through a mesh sieve to obtain straw powder; (2) calcining the straw powder at a high temperature under nitrogen protection to obtain biochar, and then putting the biochar into a ball mill for ball milling to obtain ball-milled biochar; (3) adding the straw powder into a sodium hydroxide solution for reaction, and centrifuging a reactant to obtain a viscous product; and (4) stirring the viscous product uniformly with the ball-milled biochar to obtain a mixture, pouring the mixture into a mold, and freezing and drying the mixture to obtain a seawater desalinator. The evaporator features high evaporation rate and the photothermal conversion efficiency, and the ion concentration of the water body after evaporation is greatly reduced.

Abstract: A preparation method of a crop straw-based seawater desalinator and a product thereof, and the preparation process is as follows: (1) crushing crop straws, and screening the crushed crop straws through a mesh sieve to obtain straw powder; (2) calcining the straw powder at a high temperature under nitrogen protection to obtain biochar, and then putting the biochar into a ball mill for ball milling to obtain ball-milled biochar; (3) adding the straw powder into a sodium hydroxide solution for reaction, and centrifuging a reactant to obtain a viscous product; and (4) stirring the viscous product uniformly with the ball-milled biochar to obtain a mixture, pouring the mixture into a mold, and freezing and drying the mixture to obtain a seawater desalinator. The evaporator features high evaporation rate and the photothermal conversion efficiency, and the ion concentration of the water body after evaporation is greatly reduced.

Abstract: A Streptococcus suis (S. suis) vaccine is provided. For the S. suis vaccine, an antigen is a protein with an amino acid sequence shown in SEQ ID NO: 2. A preparation method of the S. suis vaccine is provided, including the following steps: mixing a white oil and aluminum stearate to obtain a white oil adjuvant; adding poly sorbate 80 to an aqueous solution of the protein with the amino acid sequence shown in SEQ ID NO: 2, and thoroughly mixing to obtain an antigen solution; and mixing the antigen solution with the white oil adjuvant according to a volume ratio of (0.5-1.5):2, and emulsifying to obtain the S. suis vaccine. An animal immunized with the S. suis vaccine of the present disclosure can effectively resist the attack of S. suis serotype 2, 3, and 31, with a vaccine protection rate as high as 100%.

Abstract: A method for creating a new germplasm of a male sterile crop by gene editing and an application thereof are provided. In this method, the gene editing is performed on an exon region of a Ty-5 gene, and a deletion of DNA sequence is introduced by using a repair mechanism of plants themselves to double-strand breaks (DSBs), causing a loss of function of Ty-5 gene, thereby obtaining a male-sterile character. The method can be applied without being limited by crop categories. After the gene editing is performed on Ty-5 genes of various crops, new germplasms can be quickly obtained. The new germplasms have the same agronomic characters as the previous materials, and only differ in sexual aspect, which effectively solves the problem of the lack of male sterile materials and unstable fertility in natural resources.

Abstract: A pig production method by using a detachable and washable wet curtain for intensive pig farming The detachable and washable wet curtain includes a wet curtain mounting frame (1), a spray tube (4), a plurality of rotatable reels (7) equivalent to sleeves, a reel (6) with a buckle, a wet curtain net (8), a reel (10) with a buckle, and a manual handle (9). The wet curtain is formed by a low-cost common material, namely a plastic net and the like, is convenient to mount, detach, and wash, and is not prone to making dust, scale, algae, mosquitos, and the like accumulate thereon, thus reducing the replacement cost and use cost. Therefore, a new cooling method based on the wet curtain is provided for pig farms in summer, and it is also suitable for other livestock farms in summer.

Abstract: A preparation method for a combined modified straw active particulate carbon adsorption material and use of same. The preparation method for the combined modified straw active particulate carbon adsorption material comprises the following steps: 1) mixing straw powders, distilled water, a binder and a composite mineral, then pelletizing same, and then placing same in a tube furnace for pyrolysis to prepare straw particulate carbon; 2) introducing an inert gas into a modification reagent, adjusting the pH value combined and 3) soaking the straw particulate carbon into the combined modification solution for 30 min, and performing cleaning and drying, so as to obtain a combined modified straw active particulate carbon adsorption material. The combined modified straw active particulate carbon has a good adsorption effect on phosphate group in low-pollution water.

Abstract: A Bacillus altitudinis ST15 antagonizing Xanthomonas oryzae and use thereof, the Bacillus altitudinis ST15 was deposited in China General Microbiological Culture Collection Center on Jun. 28, 2020 and has the deposit number of CGMCC No. 20156. The Bacillus altitudinis has a relatively high antagonistic activity to Xanthomonas oryzae pv. oryzae and Xanthomonas oryzae pv. oryzicola, can promote growth of rice and improve a drought resistance of a rice seedling, and has a relatively strong tolerance to common chemical microbicides for controlling bacterial diseases of rice. A bacterial suspension or a biocontrol inoculant prepared from this strain has a relatively high biocontrol effect on bacterial blight of rice and bacterial leaf streak of rice, can replace or reduce chemical pesticides, improves safety of food and ecological environment, and has relatively high economic and social benefits.

Abstract: A chromosome segment derived from G. anomalum leading to a lethal phenotype in G. hirsutum, and molecular markers thereof are provided. The chromosome segment A11-9 is derived from G. anomalum, is located on chromosome 11 of a G. anomalum genome, and is marked by 6 pairs of simple sequence repeat (SSR) markers: NAU5192, A11_175, JAAS3191, A11_243, JAAS3310, and A11_1193. With DNA of G. anomalum as a template, the 6 pairs of SSR markers are used together to amplify the DNA of G. anomalum, and a chromosome segment with target fragments of the 6 pairs of SSR markers is the G. anomalum chromosome segment A11-9. A single chromosome segment introgression line derived from G. anomalum with a lethal phenotype is obtained, and the development of the single chromosome segment introgression line provides an important material for promoting the fine mapping of a target gene and the subsequent map-based cloning.

Abstract: The present invention clones a gene ZmNLP5 from maize, which plays an important regulatory role in nitrogen assimilation, and the open reading frame of which has a DNA sequence shown as SEQ ID NO:1. The transcription factor protein encoded by the ZmNLP5 gene has an amino acid sequence shown as SEQ ID NO:2. The uses of the maize NLP transcription factor ZmNLP5 mentioned above in promoting expression of a nitrogen metabolic key enzyme gene ZmNIR1.1, in promoting expression of a nitrogen metabolic key enzyme gene ZmNIR1.2, in promoting expression of a nitrogen metabolic key enzyme gene ZmNR1.1, in promoting expression of a nitrogen metabolic key enzyme gene ZmNR1.2, in improving nitrogen assimilation in maize, and in promoting elongation growth of maize root in deficient nitrogen environment are further provided.

Abstract: The present invention relates to the preparation of the compound immunopotentiator and the application thereof in a foot-and-mouth disease vaccine of pigs. According to the present invention, the foot-and-mouth disease vaccine of pigs is taken as a research subject, and on this basis, several immunopotentiators having obvious immunopotentiating effects are selected for the compound immunopotentiator, and an antigen/vaccine is mixed with the immunopotentiator to prepare a vaccine-immunized pig. An animal experiment result shows that the present invention has obvious immunopotentiating effects. After immunizing the vaccine containing the compound immunopotentiator, a window period for antibody production can be significantly shortened to 7 days; a LPB-ELISA antibody titer is significantly improved, and an antibody pass rate is significantly increased; an immune protection period is also significantly extended, at least up to 7 months.

Abstract: A high-efficiency Zea mays L. breeding method based on individual plant evaluation and genome-wide selection (GWS) includes: S1. in a first cropping season, pollinating a Zea mays L. female parent with multiple male parents; S2. in a second cropping season, subjecting hybrid seeds to single-seed sowing, conducting individual plant selection, and evaluating target traits; S3. identifying a parent of a selected cross combination; S4. subjecting a target trait of a cross combination to genome-wide prediction; S5. selecting an excellent cross combination according to a predicted target trait; and S6. subjecting the selected excellent cross combination directly to variety registration or to further evaluation. The high-efficiency Zea mays L. breeding method provided by the present disclosure greatly reduces a quantity of cross combination seeds obtained in the first cropping season and a planting scale of the cross combinations in the second cropping season, and effectively reduces the breeding cost.

Abstract: A photo-curing three-dimensional printing preview method, comprising: obtaining the relationship of the conversion rate of a photosensitive resin system versus time under different curing conditions by measuring the relationship between the absorbance of the photosensitive resin system under different curing conditions and the time; fitting on experimental curves on the basis of a photo-curing kinetic equation by an iterative solution method, and obtaining a photo-curing kinetic constant of the photosensitive resin system; solving a photo-curing reaction kinetic differential equation set by using a fixed-step Euler method, and obtaining the relationship curves of the spatial distribution of each component content of the photosensitive resin system versus time; and conducting simulation printing, in conjunction with the relationship curves obtaining a space distribution of each component content in each layer of structure in a three-dimensional printing product after printing is completed, and completing a pho

Abstract: A method for creating a new germplasm of a male sterile crop by gene editing and an application thereof are provided. In this method, the gene editing is performed on an exon region of a Ty-5 gene, and a deletion of DNA sequence is introduced by using a repair mechanism of plants themselves to double-strand breaks (DSBs), causing a loss of function of Ty-5 gene, thereby obtaining a male-sterile character. The method can be applied without being limited by crop categories. After the gene editing is performed on Ty-5 genes of various crops, new germplasms can be quickly obtained. The new germplasms have the same agronomic characters as the previous materials, and only differ in sexual aspect, which effectively solves the problem of the lack of male sterile materials and unstable fertility in natural resources.

Abstract: A method for creating a new germplasm of a male sterile crop by gene editing and an application thereof are provided. In this method, the gene editing is performed on an exon region of a Ty-5 gene, and a deletion of DNA sequence is introduced by using a repair mechanism of plants themselves to double-strand breaks (DSBs), causing a loss of function of Ty-5 gene, thereby obtaining a male-sterile character. The method can be applied without being limited by crop categories. After the gene editing is performed on Ty-5 genes of various crops, new germplasms can be quickly obtained. The new germplasms have the same agronomic characters as the previous materials, and only differ in sexual aspect, which effectively solves the problem of the lack of male sterile materials and unstable fertility in natural resources.

Abstract: The present invention provides a human-derived molecularly modified insecticidal protein, and a preparation method and application thereof, and belongs to the field of genetic engineering and biological control. The present invention provides a human-derived molecularly modified insecticidal protein, and the amino acid sequence of the insecticidal protein CCL-CCL_scFv is shown as SEQ ID No. 1. The insecticidal protein CCL-CCL_scFv shows significantly higher affinity with midgut BBMV of Plutella xylostella than Cry1Ab toxin, competes with Cry1Ab and Cry1Ac toxins for binding the midgut BBMV of Plutella xylostella, and is a mimic of Cry1Ab and Cry1Ac toxins. Through Plutella xylostella indoor insecticidal biological activity assay, the insecticidal protein shows a good insecticidal effect, and can effectively replace Cry1Ac or Cry1Ab toxin for biological control of insect pest.

Abstract: A method for creating a new germplasm of a male sterile crop by gene editing and an application thereof are provided. In this method, the gene editing is performed on an exon region of a Ty-5 gene, and a deletion of DNA sequence is introduced by using a repair mechanism of plants themselves to double-strand breaks (DSBs), causing a loss of function of Ty-5 gene, thereby obtaining a male-sterile character. The method can be applied without being limited by crop categories. After the gene editing is performed on Ty-5 genes of various crops, new germplasms can be quickly obtained. The new germplasms have the same agronomic characters as the previous materials, and only differ in sexual aspect, which effectively solves the problem of the lack of male sterile materials and unstable fertility in natural resources.

Abstract: Disclosed are the use of an Agrimonia pilosa extract in the preparation of an agent for inhibiting or reducing growth virus in tomato plants, and a method for the Agrimonia pilosa extract.