Abstract: The present invention relates to an axon bundle obtained by a method of culturing a nerve cell aggregation having one spheroid and an axon bundle extending from the spheroid wherein the nerve cell aggregation consisting of a plurality of neurons having a cell body and an axon obtained by a method comprising the following step: supplying a culture medium to one first chamber, one second chamber and one channel having the length of at least 1 mm, the width of 100 to 150 ?m and the height of 100 to 200 ?m, which connecting said first chamber and second chamber, wherein said first chamber, second chamber, and channel are contained in one of the modules disposed in a culture plate, seeding the first chamber with a nerve cell derived from a stem cell or a spheroid of primary cultured nerve cells; and culturing said nerve cell, thereby growing an axon bundle from said spheroid and extending them into said channel, wherein the length of the axon bundle obtained is 1 mm or more, and the diameter of the axon bundles

Abstract: An object of the present invention is to provide a method for inducing the formation of the presynaptic apparatus in human neurons. According to the present invention, a method for inducing the formation of the presynaptic apparatus in human neurons, comprising co-culturing the peripheral neurons with microbeads in which at least one LRRTM molecule selected from the group consisting of the LRRTM (Leucine-rich repeat transmembrane neuronal protein) family molecules or a fusion protein containing the same is fixed to the surface (wherein, the LRRTM molecule or the fusion protein containing the same molecule is fixed to the surface of the microbead via a linker), and a microbead used for the method, are provided.

Abstract: A culture method comprises: filling a first well 1 and a second well 2 in a culture vessel with a culture medium for neurons, the first well 1 and the second well 2 communicating with each other through a flow channel 3; seeding the first well 1 with neurons N; culturing axons a of the neurons N until the axons a of the neurons N reach the second well 2 through the flow channel 3 and also block the flow channel 3; and thereafter removing the culture medium in the second well 2, filling the second well 2 with a culture medium for cells to be co-cultured with the neurons N, seeding cells C to be co-cultured in the second well 2, and culturing the cells C together with the axons a of the neurons N.