Abstract: Disclosed is a pharmaceutical composition containing losartan carboxylic acid and a preparing method thereof. The pharmaceutical composition has the improved dissolution speed and dissolution rate, and the reduced variation in dissolution depending on the pH of a medium.

Abstract: Artificial modulation of ascorbate level was investigated in mice capable of de novo synthesis of ascorbate. When mice were given exogenous ascorbate or its physiological precursor, L-gulono-?-lactone, the plasma level of ascorbate was elevated substantially but immediately returned to the basal levels. Comparably, the administration of ascorbate oxidase caused a rapid disappearance of plasma ascorbate but followed by an immediate restoration of ascorbate. These results indicate the difficulties encountered in the modulation of ascorbate level in the animal. However, the circulation life of the exogenous ascorbate oxidase in the animal was successfully extended by chemical modification with methoxypolyethylene glycol. The modified enzyme retained a full activity and exerted a remarkably prolonged depletion of plasma ascorbate compared with the native enzyme.