Abstract: A method for commercial production of avidin entails heterologous expression of the protein in plants, in native conformation, at an expression level such that avidin represents at least 0.1% of total extracted protein. A genetic map of the integration locus allows for the identification of the avidin-expressing plant. Genetic loci on a plant chromosome are revealed that support high levels of avidin expression and that can be used as a site of integration for high level expression of other genes of interest.
Type:
Grant
Filed:
November 6, 1995
Date of Patent:
June 16, 1998
Assignee:
John Howard
Inventors:
Chris Baszczynski, Elizabeth Hood, Sheila Maddock, Terry EuClaire Meyer, James C. Register, III, Derrick Witcher, John A. Howard
Abstract: To permit ultrasonic leak detection, especially in internal combustion engines, an ultrasonic source is housed in a tubular body adapted for attachment to a sparking plug aperture in an engine and ultrasonic signals may thereby be injected into engine cylinders, there being an ultrasonic detector having a body with a tapered end to ensure directional limitation is used.
Type:
Grant
Filed:
November 6, 1989
Date of Patent:
January 29, 1991
Assignee:
David John Howard Peacock
Inventors:
David J. H. Peacock, Michael D. Dorsett