Abstract: A method for commercial production of avidin entails heterologous expression of the protein in plants, in native conformation, at an expression level such that avidin represents at least 0.1% of total extracted protein. A genetic map of the integration locus allows for the identification of the avidin-expressing plant. Genetic loci on a plant chromosome are revealed that support high levels of avidin expression and that can be used as a site of integration for high level expression of other genes of interest.
Type:
Grant
Filed:
November 6, 1995
Date of Patent:
June 16, 1998
Assignee:
John Howard
Inventors:
Chris Baszczynski, Elizabeth Hood, Sheila Maddock, Terry EuClaire Meyer, James C. Register, III, Derrick Witcher, John A. Howard