Abstract: The present invention relates to an improved process for producing hydroxynitrile lyases. A process is provided whereby hydroxynitrile lyases may be produced by cultivation in bacteria or suitable host cells. Cells containing a gene encoding for a hydroxynitrile lyase may be precultivated, induced with a low concentration of IPTG, cultured, and lysed. The process allows for the production of relatively large quantities of hydroxynitrile lyases in a dissolved, native, and active form, and without the presence of substantial amounts of inclusion bodies, and thus allowing for the production of the lyases without requiring expensive and time consuming renaturation steps.
Type:
Grant
Filed:
December 27, 2000
Date of Patent:
March 27, 2007
Assignee:
Julich Chiral Solutions GmbH
Inventors:
Franz Effenberger, Harald Wajant, Siegfried Förster