Patents Assigned to Juno Therapeutics, Inc.
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Patent number: 12268741Abstract: Provided are receptor tyrosine kinase-like orphan receptor 1 (ROR1)-binding molecules, in particular, to human antibodies specific for ROR1, including antibody fragments. The present disclosure further relates to recombinant receptors, including chimeric antigen receptors (CARs) that contain such antibodies or fragments, and polynucleotides that encode the antibodies, antigen-binding fragments or receptors specific for ROR1. The disclosure further relates to genetically engineered cells, containing such ROR1-binding proteins and receptors, and related methods and uses thereof in adoptive cell therapy.Type: GrantFiled: January 28, 2020Date of Patent: April 8, 2025Assignee: Juno Therapeutics, Inc.Inventors: Andreia Costa, Rupesh Amin, Jenna Bailey, Samriti Bedi, Brian Belmont, Aye Chen, Stephen Jacob Goldfless, Eric Jeffery, Yue Jiang, Yeonjoo Oh, Madeline Williams, Collin Hauskins, Catherine Sierra
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Patent number: 12263190Abstract: The present disclosure relates in some aspects to methods, compositions and uses involving immunotherapies, such as adoptive cell therapy, e.g., T cell therapy, and an immunomodulatory compound, such as a structural or functional analog or derivative of thalidomide and/or an inhibitor of E3-ubiquitin ligase. The provided methods, compositions and uses include those for combination therapies involving the administration or use of one or more immunomodulatory compounds in conjunction with a T cell therapy, such as a genetically engineered T cell therapy involving cells engineered with a recombinant receptor, such as chimeric antigen receptor (CAR)-expressing T cells. Also provided are compositions, methods of administration to subjects, articles of manufacture and kits for use in the methods.Type: GrantFiled: November 7, 2019Date of Patent: April 1, 2025Assignee: Juno Therapeutics, Inc.Inventors: Jens Hasskarl, Stanley R. Frankel, Michael Ports, Michael Pourdehnad, Heidi Jessup, Yue Jiang, Jim Shi Xiang Qin, Neha Soni, Melissa Works
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Patent number: 12264371Abstract: Provided are methods of detecting replication competent virus, e.g., replication competent retrovirus such as gammaretrovirus or lentivirus, in a sample containing a cell transduced with a viral vector particle encoding a recombinant and/or heterologous molecule, e.g., heterologous gene product. The methods may include assessing transcription of one or more target genes, such as viral genes, that are expressed in a retrovirus but not expressed in the viral vector particle. Replication competent retrovirus may be determined to be present if the levels of RNA of the one or more target genes is higher than a reference value, which can be measured directly or indirectly, including from a positive control sample containing RNA from the respective target gene at a known level and/or at or above the limit of detection of the assay.Type: GrantFiled: November 22, 2022Date of Patent: April 1, 2025Assignee: Juno Therapeutics, Inc.Inventors: Ruth Berry, Edwin Webb
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Publication number: 20250101379Abstract: Provided are methods of continuous counterflow centrifugation for the manufacturing of cell compositions, including for the production of T cell therapies including cells that express recombinant receptors such as chimeric antigen receptors (CARs).Type: ApplicationFiled: January 27, 2023Publication date: March 27, 2025Applicant: Juno Therapeutics, Inc.Inventors: Calvin CHAN, Chin-Wei LIM
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Patent number: 12258580Abstract: The present disclosure provides methods for genetically engineering T cells, such as CD4+ T cells, for use in cell therapy. In some aspects, the provided methods include one or more steps for incubating the cells under stimulating conditions, introducing a recombinant polypeptide to the cells through transduction or transfection, and cultivating the cells under conditions that promote proliferation and/or expansion. In some aspects, the incubation and/or the cultivation is performed in the presence of recombinant IL-2. In some aspects, the provided methods are an efficient, reliable means to produce genetically engineered T cells with a high degree of success.Type: GrantFiled: October 31, 2018Date of Patent: March 25, 2025Assignee: Juno Therapeutics, Inc.Inventors: Sarah Y. Lee, Pascal Beauchesne, Mark L. Bonyhadi, Ryan L. Crisman, Ryan P. Larson, Mary Mallaney, Christopher Glen Ramsborg, Clinton Weber, John Matthew Wesner, Nathan Yee
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Publication number: 20250090542Abstract: Provided herein are combination therapies involving administration of an immunotherapy involving a cell therapy, such as a T cell therapy, and an inhibitor of gamma secretase. Also provided are methods for engineering, preparing, and producing the cells, compositions containing the cells and/or gamma secretase inhibitor, and kits and devices containing and for using, producing and administering the cells and/or gamma secretase inhibitor, such as in accord with the provided combination therapy methods.Type: ApplicationFiled: November 27, 2024Publication date: March 20, 2025Applicants: Juno Therapeutics, Inc., Fred Hutchinson Cancer CenterInventors: Damian J. GREEN, Stanley R. RIDDELL, Melissa WORKS
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Publication number: 20250059253Abstract: Provided are methods, compositions and articles of manufacture for use in cell therapy involving the administration of one or more doses of a therapeutic T cell composition, and methods, compositions and articles of manufacture for use in the same. The cells of the T cell composition express recombinant receptors such as chimeric receptors, e.g. chimeric antigen receptors (CARs) or other transgenic receptors such as T cell receptors (TCRs). Features of the embodiments of the present disclosure, including the dose of cells or units of cells administered and/or the phenotype of administered cells, provide various advantages, such as consistent dosing, lower risk of toxicity and/or increased response in subjects administered the T cell compositions.Type: ApplicationFiled: November 1, 2024Publication date: February 20, 2025Applicant: Juno Therapeutics, Inc.Inventors: Kedar Himanshu DAVE, Todd DEVRIES, Ronald James HAUSE, JR., Ryan P. LARSON, Christopher Glen RAMSBORG, Claire L. SUTHERLAND, Nathan K. YEE, Rachel K. YOST
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Patent number: 12215348Abstract: Provided herein are methods and compositions for generating engineered cells, such as cells expressing a recombinant receptor, including methods involving stimulation and/or engineering of an input composition having a defined ratio of naïve-like CD4+ T cells to naïve-like CD8+ T cells. In particular, the methods can be used to engineer T cells with genetically engineered receptors, such as genetically engineered antigen receptors such as engineered (recombinant) TCRs and chimeric antigen receptors (CARs), or other recombinant chimeric receptors. Features of the methods include producing a more consistent and/or predictable T cell product and/or a product with lower toxicity compared with other methods.Type: GrantFiled: November 10, 2023Date of Patent: February 4, 2025Assignee: Juno Therapeutics, Inc.Inventors: Mirna Mujacic, Ayu Rahardjo
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Patent number: 12208137Abstract: The present disclosure relates in some aspects to methods, cells, and compositions for preparing cells and compositions for genetic engineering and cell therapy. Provided in some embodiments are streamlined cell preparation methods, e.g., for isolation, processing, incubation, and genetic engineering of cells and populations of cells. Also provided are cells and compositions produced by the methods and methods of their use. The cells can include immune cells, such as T cells, and generally include a plurality of isolated T cell populations or types. In some aspects, the methods are capable of preparing of a plurality of different cell populations for adoptive therapy using fewer steps and/or resources and/or reduced handling compared with other methods.Type: GrantFiled: February 8, 2023Date of Patent: January 28, 2025Assignee: Juno Therapeutics, Inc.Inventors: Chris Ramsborg, Mark L. Bonyhadi, Calvin Chan, Pascal Beauchesne
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Publication number: 20250027869Abstract: Provided are methods of detecting, assessing or determining the presence or absence of particles, such as bead particles, present in a cell composition. Also provided are articles of manufacture and kits for use in the methods.Type: ApplicationFiled: October 7, 2024Publication date: January 23, 2025Applicant: Juno Therapeutics, Inc.Inventors: Brittney Jo LIVINGSTON, Hai YUE
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Patent number: 12193994Abstract: Provided herein are combination therapies involving administration of an immunotherapy involving a cell therapy, such as a T cell therapy, and an inhibitor of gamma secretase. Also provided are methods for engineering, preparing, and producing the cells, compositions containing the cells and/or gamma secretase inhibitor, and kits and devices containing and for using, producing and administering the cells and/or gamma secretase inhibitor, such as in accord with the provided combination therapy methods.Type: GrantFiled: November 6, 2018Date of Patent: January 14, 2025Assignees: Juno Therapeutics, Inc., Fred Hutchinson Cancer CenterInventors: Damian J. Green, Stanley R. Riddell, Melissa Works
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Publication number: 20250011387Abstract: Provided are adoptive cell therapy methods involving the administration of doses of cells for treating disease and conditions, including certain B cell malignancies. The cells generally express recombinant receptors such as chimeric antigen receptors (CARs). In some embodiments, the methods are for treating subjects with non-Hodgkin lymphoma (NHL). In some embodiments, the methods are for treating subjects with relapsed or refractory NHL. Also provided are articles of manufacture and prophylactic treatments in connection with adoptive therapy methods.Type: ApplicationFiled: September 11, 2024Publication date: January 9, 2025Applicant: Juno Therapeutics, Inc.Inventors: Tina ALBERTSON, Brian CHRISTIN, Jacob Randolph GARCIA, Christopher Glen RAMSBORG, Claire L. SUTHERLAND, Clinton WEBER, Rachel K. YOST, Mark J. GILBERT, He LI
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Patent number: 12168027Abstract: Provided herein are methods and articles of manufacture for use with cell therapy for the treatment of diseases or conditions, e.g., cancer, including for predicting and treating a toxicity. In some embodiments, the toxicity is related to cytokine release syndrome (CRS). The methods generally involve assessing a change in a factor indicative of tumor burden in a subject that is associated with and/or correlate to a risk of developing toxicity. In some aspects, the methods can be used to determine if the subject is at risk or likely at risk for developing a toxicity following administration of the cell therapy. Also provided are methods for treating a subject having a disease or condition, in some cases involving administration of the cell therapy, based on assessment of risk of developing a toxicity following administration of the therapy. Also provided herein are reagents and kits for performing the methods.Type: GrantFiled: December 16, 2022Date of Patent: December 17, 2024Assignee: Juno Therapeutics, Inc.Inventors: Tina Albertson, Jacob Randolph Garcia, He Li
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Patent number: 12163952Abstract: Provided herein are methods, compositions and articles of manufacture for use in connection with cell therapy involving the administration of one or more doses of a therapeutic T cell composition. The cells of the T cell composition express recombinant receptors such as chimeric receptors, e.g. chimeric antigen receptors (CARs) or other transgenic receptors such as T cell receptors (TCRs). Features of the provided embodiments, including the numbers of cells or units of cells administered and/or the potency of administered cells, provide various advantages, such as lower risk of toxicity in subjects administered the T cell compositions.Type: GrantFiled: February 27, 2018Date of Patent: December 10, 2024Assignee: Juno Therapeutics, Inc.Inventors: Brian Christin, Michael Gerard Covington, Kedar Himanshu Dave, Richard James Getto, Jr., Tom Kowski, Ryan P. Larson, Christopher Glen Ramsborg, Nikolaus Sebastian Trede, Clinton Weber, James Boyd Whitmore, Nathan Yee, Pascal Beauchesne, Travis Beckett, Samuel Charles Blackman, Nathaniel Chartrand, Mel Davis-Pickett, Mark Gilbert, Nathaniel Lambert, He Li, Mary Mallaney, Kathryn Lindsay Pollock, Valerie Odegard, Jeff Smith, Claire Sutherland, Andrew W. Walker
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Patent number: 12161670Abstract: Provided are methods, compositions and articles of manufacture for use in cell therapy involving the administration of one or more doses of a therapeutic T cell composition, and methods, compositions and articles of manufacture for use in the same. The cells of the T cell composition express recombinant receptors such as chimeric receptors, e.g. chimeric antigen receptors (CARs) or other transgenic receptors such as T cell receptors (TCRs). Features of the embodiments of the present disclosure, including the dose of cells or units of cells administered and/or the phenotype of administered cells, provide various advantages, such as consistent dosing, lower risk of toxicity and/or increased response in subjects administered the T cell compositions.Type: GrantFiled: December 7, 2018Date of Patent: December 10, 2024Assignee: Juno Therapeutics, Inc.Inventors: Kedar Himanshu Dave, Todd Devries, Ronald James Hause, Jr., Ryan P. Larson, Christopher Glen Ramsborg, Claire L. Sutherland, Nathan K. Yee, Rachel K. Yost
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Patent number: 12140526Abstract: Provided are methods of detecting, assessing or determining the presence or absence of particles, such as bead particles, present in a cell composition. Also provided are articles of manufacture and kits for use in the methods.Type: GrantFiled: February 28, 2019Date of Patent: November 12, 2024Assignee: Juno Therapeutics, Inc.Inventors: Brittney Jo Livingston, Hai Yue
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Patent number: 12139526Abstract: Provided are chimeric receptors for engineering cells for adoptive therapy, including T cells, and the genetically engineered cells. In some embodiments, the chimeric receptors, such as chimeric antigen receptors (CARs) are modified in a junction region by one or more amino acid modifications such that peptide fragments of such region exhibit a lower binding affinity for a human leukocyte antigen (HLA) and/or the region exhibits reduced immunogenicity, including following administration to a subject. In some aspects, also provided are methods and compositions for engineering and producing cells expressing such chimeric receptors, compositions containing the cells, and method for their administration to subjects. In some embodiments, features of the chimeric receptors and engineered cells containing the chimeric receptors result in methods that provide for increased or improved activity, efficacy and/or persistence.Type: GrantFiled: December 2, 2016Date of Patent: November 12, 2024Assignee: Juno Therapeutics, Inc.Inventors: Lucas James Thompson, Robert F. Dubose, Archana Brahmandam
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Publication number: 20240368625Abstract: Provided are methods for preparing T cells for cell therapy, compositions produced by the methods, and methods of administering the cells to subjects. In particular, the disclosure relates to preparation of engineered T cells, such as those expressing genetically engineered receptors, such as genetically engineered antigen receptors such as engineered (recombinant) TCRs and chimeric antigen receptors (CARs), or other recombinant chimeric receptors. Features of the methods include producing a more consistent and/or predictable T cell product and/or lower toxicity compared with other methods. The provided methods include incubating cells under stimulating conditions to induce expansion or proliferation of naïve-like T cells compared to non-naïve like T cells in the stimulated composition, which in turn can result in preferential transduction of cells derived from the naïve-like T cells.Type: ApplicationFiled: March 26, 2024Publication date: November 7, 2024Applicant: Juno Therapeutics, Inc.Inventor: Mark L. BONYHADI
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Publication number: 20240302349Abstract: Provided are methods and articles of manufacture for use with cell therapy for the treatment of diseases or conditions, e.g., cancer, including for predicting likelihood of the subject responding to a therapy, such as a cell therapy, e.g., a chimeric antigen receptor (CAR) T cell therapy. In some aspects, the predicting is based on detecting certain biomarkers of immune cells associated with and/or that correlate with response following administration of the therapy. The methods generally involve detecting a marker by assaying a biological sample from a subject that is a candidate for treatment, optionally with a cell therapy, to determine if the subject is likely to respond to the therapy. The present disclosure also provides methods for treating a subject having a disease or condition, in some cases involving administration of the cell therapy, based on assessment the biomarker. Also provided herein are reagents and kits for performing the methods.Type: ApplicationFiled: May 24, 2024Publication date: September 12, 2024Applicant: Juno Therapeutics, Inc.Inventors: Trevor DO, Howard STERN, Christina SWANSON
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Publication number: 20240285762Abstract: Provided herein are adoptive cell therapy methods and uses involving the administration of a dose of T cells expressing a CD19-directed chimeric antigen receptor for treating subjects with Systemic autoimmune disease and related methods, compositions, uses and articles of manufacture.Type: ApplicationFiled: February 28, 2024Publication date: August 29, 2024Applicant: Juno Therapeutics, Inc.Inventors: Michael MALDONADO, Nikolay DELEV, Ashley KOEGEL, Susana BARRIGA FALCON, Steven Marc GREENBERG, Michael BURGESS