Abstract: A method for reducing the occurrence of falsely elevated results in a peroxidase-catalyzed, enzyme assay is described. Interference in an assay caused by blood or bood products in a clinical specimen is eliminated or reduced by reacting the specimen with an oxidizing agent such as sodium hypochlorite, hydrogen peroxide or sodium meta-periodate.

Abstract: An immunoassay procedure for the detection of chlamydia trachomatis antigen in a urogenital clinical specimen including a method for substantially eliminating the occurrence of false negative and false positive results of the immunoassay procedure. The method comprises treating a patent specimen with an aqueous solution having a final concentration of 0.1M NaOH or 0.1M KOH and then neutralizing the specimen-containing solution before conducting the immunoassay.

Abstract: The storage stability of peroxidase-reactive luminescent cocktails which employ chemiluminescent 2,3-dihydro-1,4-phthalazinediones, oxidants reactant therewith to cause light emission in peroxidase-mediated luminescent reactions, and phenolic sensitivity enhancers is improved by maintaining the pH of the cocktail prior to use at a value in the range of about 3 to about 6 and preferably at a pH of about 5. An analyte sample may be subjected to a reaction resulting in a peroxidase-containing phase, the peroxidase content of which is related to the quantity of analyte in the sample, and the peroxidase phase may then be combined with the thus-described cocktail and an aqueous alkaline organic buffer to raise the pH of the resulting reaction mixture to a value in the range of 7-9, favoring light emission.