Abstract: Radionuclide labelled conjugates based on substance P and analogues thereof with the chelator (DOTAGA) (1-(1-rarboxy-3-carboxy-propyl)-4,7,10(carboxymethyl)-1,4,7,10-tetraazacyclo-dodecane, DOTASA (1-(1-carboxy-2carboxy-ethyl)-4,7,10(carboxymethyl)1,4,7,10-tetraazacyclo-dodecane, or chelator (DOTA) [1,4,7,10 tetraazaacyclo-dodecane 1,4,7,10-tetra(acetic acid)] are useful radiopharmaceutical substances for targeting and treatment of brain tumors, especially gliomas.

Abstract: Tissue engineering is the development of biological substitutes to restore, maintain, or improve tissue function. One strategy that has been created to regenerate new tissue comprising the steps of providing cells, expanding the isolated cells in a first culture medium in which the cells lack differentiated functions and redifferentiating the expanded cells in a second cell culture medium. The present invention provides an improved method for tissue engineering. In particular, the method provides specific biochemical factors to supplement cell culture medium during the redifferentiation process with the goal of regenerating tissue equivalents that resemble natural tissues both structurally and functionally. These specific chemical factors induce and/or accelerate and/or promote the redifferentiation of the previously expanded cells.