Abstract: Provided herein are direct-to-library methods, systems, and compositions.
Type:
Application
Filed:
May 18, 2021
Publication date:
January 13, 2022
Applicant:
KARIUS, INC.
Inventors:
Sivan BERCOVICI, Lily BLAIR, Timothy A. BLAUWKAMP, Peter J. EUGSTER, David K. HONG, Trupti KAWLI, Michael J. ROSEN, Damek SPACEK, Igor D. VILFAN
Abstract: Provided herein are fragment length profiles of nucleic acid libraries, methods of generating fragment length profiles of nucleic acid libraries and methods of using fragment length profiles for diagnostics and/or prognostics. The application further provides methods, compositions and kits for determining the infection stage or the site of localization in a subject.
Type:
Application
Filed:
May 18, 2021
Publication date:
December 30, 2021
Applicant:
KARIUS, INC.
Inventors:
Sivan BERCOVICI, Lily BLAIR, Timothy A. BLAUWKAMP, Peter J. EUGSTER, Desiree HOLLEMON, David K. HONG, Trupti KAWLI, Mark Alec KOWARSKY, Martin M.S. LINDNER, Michael J. ROSEN, Damek SPACEK, Igor D. VILFAN
Abstract: This disclosure provides methods, compositions and kits for determining if nucleic acids detected in a sample such as a clinical sample are derived from contaminant pathogens or clinically-relevant pathogens.
Type:
Application
Filed:
May 27, 2020
Publication date:
September 17, 2020
Applicant:
KARIUS, INC.
Inventors:
Timothy A. BLAUWKAMP, Liza HUIJSE, Michael ROSEN, Igor D. VILFAN
Abstract: The disclosure provides methods, compositions, systems, and kits for the concurrent detection and analysis of different structural and chemical forms of nucleic acids in a sample.
Type:
Grant
Filed:
April 12, 2018
Date of Patent:
June 30, 2020
Assignee:
KARIUS, INC.
Inventors:
Timothy A. Blauwkamp, Rene Sit, Igor D. Vilfan
Abstract: This disclosure provides methods for determining relative abundance of one or more non-host species in a sample from a host. Also provided are methods involving addition of known concentrations of synthetic nucleic acids to a sample and performing sequencing assays to identify non-host species such as pathogens. Also provided are methods of tracking samples, tracking reagents, and tracking diversity loss in sequencing assays.
Type:
Grant
Filed:
March 24, 2017
Date of Patent:
May 22, 2018
Assignee:
KARIUS, INC.
Inventors:
Fred C. Christians, Igor D. Vilfan, Michael Kertesz, Timothy A. Blauwkamp, Shivkumar Venkatasubrahmanyam, Michael Rosen, Rene Sit