Abstract: The invention is directed to methods for culturing cells so that the cells are induced to differentiate into cells that express hepatocyte phenotypes and hepatocyte progenitor phenotypes. More particularly, the invention relates to methods for culturing cells so that the cells are induced to differentiate into cells that express a definitive endodermal phenotype, a liver-committed endodermal phenotype, a hepatoblast phenotype, and hepatocyte phenotype. The invention is also directed to cells produced by the methods of the invention. The cells are useful, among other things, for treatment of liver deficiency, liver metabolism studies, and liver toxicity studies.
Type:
Application
Filed:
October 31, 2008
Publication date:
November 24, 2011
Applicant:
Kathoiieke Universitiet Leuven
Inventors:
Karen Pauwelyn, Catherine M. Verfaillie