Abstract: A tamper evident bag having a non-reusable frangible fastener profile assembly comprising two sets of lockable fastener profile elements specially constructed to manually lock together without the use of tools, and which once locked cannot be forcibly pulled apart. The bag includes a frangible connector bridge which will break away when the bag is opened indicating that the bag has been tampered with. The construction of the fastener elements also assures proper alignment of the lockable fastener profile elements across the length of the upper portion of the bag wall panels, making interlocking closure easy when pressure is applied on the outer walls of the bag behind the fastener profile elements.