Abstract: An active matrix microfluidic platform employs thin film transistor active (“TFT”) matrix liquid crystal display technology to manipulate small samples of fluid for chemical, biochemical, or biological assays without moving parts, for example, using a two-dimensional matrix array of drive electrodes. The active matrix microfluidic platform may employ existing active matrix addressing schemes and/or commercial “off-the-shelf” animation software to program assay protocols. A feedback subsystem may determine an actual location of a fluid in the microfluidic structure, and provides location information to for display, for example, on an active matrix display, and/or to control movement of one or more fluid bodies in the microfluidic structure.

Abstract: The present invention provides methods and compositions for nucleic acid methylation analysis using nicking agents.

Abstract: The present invention provides methods and compositions for exponential amplification of nucleic acid molecules using nicking agents. In certain aspects, the amplification may be performed isothermally. This invention is useful in many areas such as disease diagnosis.

Abstract: The present invention provides human tyrosine-DNA phosphodiesterases (TDPs). In particular, the present invention provides novel recombinant nucleic acids and proteins, including mutant TDPs, vectors, and TDP-producing cells, as well as co-factors for enzyme activity. The present invention further provides methods for high through-put enzymatic assay systems utilizing the TDPs of the present invention.

Abstract: The present invention provides methods and kits for amplifying target nucleic acids (including whole genomes) using nicking agents. In certain aspects, the amplification does not require the use of any external oligonucleotide primers that are capable of annealing to a portion of the target nucleic acid. This invention is useful in many areas such as genetic disease diagnoses, forensic analyses and palcoarcheological studies.

Abstract: The present invention provides methods and composition for exponential nucleic acid amplification using nicking agents. The invention is useful in many areas such as disease diagnosis, genetic variation detection and pre-mRNA alternative splicing analysis.