Abstract: The invention described herein relates to a method for combining antigen fragments of Toxoplasma gondii proteins, in the form of chimeric fusion products, and their use as diagnostic and immunogenic agents.
Type:
Application
Filed:
July 1, 2010
Publication date:
February 3, 2011
Applicant:
KENTON S.R.L.
Inventors:
NICOLA GARGANO, ELISA BEGHETTO, ANDREA SPADONI
Abstract: The invention described herein relates to a method for combining antigen fragments of Toxoplasma gondii proteins, in the form of chimeric fusion products, and their use as diagnostic and immunogenic agents.
Type:
Grant
Filed:
February 27, 2006
Date of Patent:
September 7, 2010
Assignee:
Kenton S.R.L.
Inventors:
Nicola Gargano, Elisa Beghetto, Andrea Spadoni
Abstract: The invention described herein relates to a method for identifying antigen fragments of Toxoplasma gondii proteins, and their use as diagnostic and immunogenic agents. Said method is implemented by means of selection of DNA fragments libraries of the parasite with sera of subjects who have been infected, using the phage display technique, and is characterised in that it uses the expression/exposure vector ?KM4. The method allows also to identify antigen fragments related to the time of the infection.
Type:
Grant
Filed:
March 18, 2003
Date of Patent:
February 13, 2007
Assignee:
Kenton S.R.L.
Inventors:
Nicola Gargano, Elisa Beghetto, Manlio Di Cristina, Franco Felici
Abstract: A method is described for the identification of specific tumor antigens by means of the selection of cDNA display libraries by using sera, characterised in that said selection is accomplished with the phage display technique, and in particular said selection is accomplished by means of the SEREX technique (serological analysis of autologous tumor antigens through the expression of recombinant cDNA). The method according to the invention described herein advantageously combines the SEREX approach with the potency of the phage display technique defined above, at the same time avoiding the drawbacks characteristic of the SEREX technique. The so identified antigens are useful for the preparation of medicaments for the treatment of tumors.