Abstract: A method for measuring an analyte is described that includes the steps of: i) preparing a reagent (D) in which an enzyme (A) and an enzyme (B) coexist in the absence of the analyte; ii) bringing the analyte into contact with the enzyme (A) and the enzyme (B) so that the enzyme (A) acts on the analyte to produce a product (E), on which the enzyme (B) does not substantially act, from the analyte; iii) producing a product (C) by allowing the enzyme (A) or an enzyme (F) that is different from the enzyme (A) that acts on the analyte to produce a product (C) to act on the analyte and/or the product (E); and iv) detecting the product (C) by the enzyme (B).
Type:
Grant
Filed:
March 11, 2013
Date of Patent:
August 12, 2014
Assignees:
ARKRAY, Inc., Kikkoman Biochemifa Company
Inventors:
Koji Sugiyama, Satoshi Yonehara, Kazuhiko Shimoji
Abstract: A method for measuring an analyte is described that includes the steps of: i) preparing a reagent (D) in which an enzyme (A) and an enzyme (B) coexist in the absence of the analyte; ii) bringing the analyte into contact with the enzyme (A) and the enzyme (B) so that the enzyme (A) acts on the analyte to produce a product (E), on which the enzyme (B) does not substantially act, from the analyte; iii) producing a product (C) by allowing the enzyme (A) or an enzyme (F) that is different from the enzyme (A) that acts on the analyte to produce a product (C) to act on the analyte and/or the product (E); and iv) detecting the product (C) by the enzyme (B).
Type:
Application
Filed:
March 11, 2013
Publication date:
September 19, 2013
Applicants:
KIKKOMAN BIOCHEMIFA COMPANY, ARKRAY, INC.
Inventors:
Koji Sugiyama, Satoshi Yonehara, Kazuhiko Shimoji