Abstract: The present invention discloses a heparin nano drug carrier system loaded with an amino anti-tumor drug. The drug carrier system is a conjugate formed by loading the amino anti-tumor drug on a PEGylated heparin molecule. A natural polysaccharide heparin that is biodegradable and has good compatibility and high availability is used as a drug carrier, and by combining PEG modification and the amino anti-tumor drug, nanoparticles have a significantly enhanced anti-tumor therapeutic index and biological safety in in-vivo therapy when compared with free drugs.