Abstract: Expression vectors and methods of protein purification, which allow for selection of full length protein over truncated forms of the protein being purified, are disclosed. The methods express a target protein as a three domain fusion, represented by formula I:A-[Li]-B-[L2]-C, where A is a first purification tag domain, C is the second purification tag domain and B is the target protein domain. A, B and C are preferably covalently linked by linkers, L1 and L2 may be optional. The purification tags at the N and C termini are different. The purification tags at the N and C termini are different. Expression vectors including nucleic acid sequences which encode the fusion protein represented by formula I are also disclosed. The vectors are used with host expression systems such as insect, yeast, or mammalian cells to express the target protein, which is subsequently purified as a function of the different affinity tags.

Abstract: A method for manufacturing a soft stamp includes providing a substrate having a first electrode and a second electrode, the second electrode being formed at a distance less than 100 nm from the first electrode so that a nanogap Ng is formed between the first and second electrodes; pouring a curable substance over the first and second electrodes and into the nanogap Ng; curing the curable substance to form a soft stamp; and removing the soft stamp from the first and second electrodes. The soft stamp has a nano-feature having a size less than 100 nm.

Abstract: A functionalized fibrous hierarchical zeolite includes a framework comprising aluminum atoms, silicon atoms, and oxygen atoms, the framework further comprising a plurality of micropores and a plurality of mesopores. The functionalized fibrous hierarchical zeolite is functionalized with at least one terminal amine bonded to a silicon atom. Terminal organometallic functionalities are bonded to a nitrogen atom of the at least one terminal amine, the terminal organometallic functionalities comprising a platinum atom.

Abstract: A method may include obtaining fracture image data regarding a geological region of interest. The method may further include determining various fractures in the fracture image data using a first artificial neural network and a pixel-searching process. The method may further include determining a fracture model using the fractures, a second artificial neural network, and borehole image data. The method may further include determining various fracture permeability values using the fracture model and a third artificial neural network. The method may further include determining various matrix permeability values for the geological region of interest using core sample data. The method may further include generating a coarsened grid model for the geological region of interest using a fourth artificial neural network, the matrix permeability values, and the fracture permeability values.

Abstract: A method for determining tree infestation includes placing an optical fiber around a trunk of a tree; recording with a distributed acoustic sensor (DAS) box a Rayleigh signal reflected from the tree, along the optical fiber; processing the Rayleigh signal to obtain a processed signal; calculating a signal-to-noise ratio (SNR) of the processed signal for the tree; and comparing the SNR to a threshold value and counting an alarm if the SNR is larger than the threshold value. The SNR is defined as a ratio between (1) a maximum value of a processed signal and (2) a minimum value of the processed signal.

Abstract: A method for forming CsPbBr3 perovskite nanocrystals into a two-dimensional (2D) nanosheet includes providing CsPbBr3 perovskite nanocrystals; mixing the CsPbBr3 perovskite nanocrystals into a mixture of a first solvent and a second solvent, to form a solution of the CsPbBr3 perovskite nanocrystals, the first solvent, and the second solvent; and forming an optoelectronic device by patterning the CsPbBr3 perovskite nanocrystals into a nanosheet, between first and second electrodes. The first solvent is selected to evaporate before the second solvent.

Abstract: A blend for lubricating a surface includes a lubricant and a polyepoxide terpolymer additive mixed with the lubricant. The polyepoxide terpolymer additive includes a first block having a group R1, a second block having a group R2, and a third block having a group R3. Group R1 includes C4H9 or C6H13, group R2 includes CH3, and group R3 includes C6H5.

Abstract: A functionalized fibrous hierarchical zeolite includes a framework comprising aluminum atoms, silicon atoms, and oxygen atoms, the framework further comprising a plurality of micropores and a plurality of mesopores. The functionalized fibrous hierarchical zeolite is functionalized with at least one amine. A plurality of nanoparticles comprising nickel are immobilized on the framework.

Abstract: A visible-light communication and illumination array includes a substrate and plural surface-emitting superluminescent diodes, SLDs, distributed across the substrate. A first set of SLDs of the plural SLDs generates a first light beam having substantially a first wavelength, a second set of SLDs of the plural SLDs generates a second light beam having substantially a second wavelength, and a third set of SLDs of the plural SLDs generates a third light beam having substantially a third wavelength. The array further includes a controller configured to encode at least one of the first light beam, the second light beam and the third light beam to transmit information. A combination of the first light beam, the second light beam and the third light beam produces white light.

Abstract: Modified zeolites may include a microporous framework including a plurality of micropores having diameters of less than or equal to 2 nm. The microporous framework may include at least silicon atoms and oxygen atoms. The modified zeolite may include a plurality of mesopores having diameters of greater than 2 nm and less than or equal to 50 nm, wherein the plurality of mesopores are ordered with cubic symmetry. The modified zeolite may include a plurality of zirconium hydride moieties each bonded to at least two bridging oxygen atoms, wherein a zirconium atom of the zirconium hydride is bonded to the bridging oxygen atom, and wherein the bridging oxygen atom bridges the zirconium atom of the zirconium hydride moiety and a silicon atom of the microporous framework.

Abstract: Described herein are zeolite Beta particles with radially arranged mesopores and methods of making the same. In one or more embodiments, a zeolite Beta particle may include a Beta zeolitic framework including a plurality of micropores having diameters of less than or equal to 2 nm. In embodiments, the Beta zeolitic framework may include alumina and silica. In embodiments, the zeolite Beta particles disclosed herein may include a plurality of mesopores having diameters of greater than 2 nm and less than or equal to 50 nm. In embodiments, the plurality of mesopores may be arranged in a center-radial configuration, such that mesopores run from a central region of the zeolite Beta particle towards the edge of the zeolite Beta particle.

Abstract: A method for water drainage of a substrate includes creating, on a surface of the substrate, a designated hydrophobic region having hydrophobic surfaces of a hydrophobic film. Electronic circuitries are fabricated in the designated hydrophobic region of the substrate. The method further includes creating, on the surface of the substrate, a designated hydrophilic region having hydrophilic surfaces of the substrate. A drainage channel is formed in the designated hydrophilic region. The method further includes facilitating, based on capillary imbibition of the drainage channel, fluid flow from the designated hydrophobic region to a drainage/evaporation port to prevent damage of the electronic circuitries by moisture accumulation in the designated hydrophobic region.

Abstract: Modified zeolites may include a microporous framework including a plurality of micropores having diameters of less than or equal to 2 nm. The microporous framework may include at least silicon atoms and oxygen atoms. The modified zeolite may include a plurality of mesopores having diameters of greater than 2 nm and less than or equal to 50 nm, wherein the plurality of mesopores are ordered with cubic symmetry. The modified zeolite may include a plurality of hafnium hydride moieties each bonded to at least two bridging oxygen atoms, wherein a hafnium atom of the hafnium hydride is bonded to the bridging oxygen atom, and wherein the bridging oxygen atom bridges the hafnium atom of the hafnium hydride moiety and a silicon atom of the microporous framework.

Abstract: A method for making a metal carbide based catalyst for crude oil cracking includes mixing a clay with a phosphorous based stabilizer material to obtain a liquid slurry; adding an aluminosilicate zeolite and an ultrastable Y zeolite to the liquid slurry; adding Al2Cl(OH)5 to the liquid slurry; adding metal carbide particles, having a given diameter, to the liquid slurry to obtain a mixture; and spray drying the mixture to obtain the metal carbide based catalyst. The metal carbide particles are coated with the aluminosilicate zeolite and the ultrastable Y zeolite.

Abstract: Methods for detecting His-tagged proteins using metal ion-chelating nitrilotriacetate (NTA) probes and polyacrylamide gel electrophoresis (PAGE) are disclosed. In one embodiment, the method includes using a metal ion-loaded NTA probe coupled to a UV-excitable fluorophore with visible emission and the presence of His-tagged proteins in the sample is determined by exposing the gel following PAGE, to a UV-light source with naked human eye or bench camera visualization. The metal ion-loaded NTA-containing chelator head can be coupled to a fluorophore that is not UV-excitable (i.e., with the majority of emission and excitation in the visible region of the electromagnetic spectrum. The method includes separating proteins in a sample using PAGE, contacting the gel following electrophoresis with a composition containing a metal ion-loaded NTA probe coupled to the fluorophore, to allow binding of the probe to the His-tagged proteins, and detecting the presence of the probe and therefore of the His-tagged proteins.

Abstract: A light-emitting device includes doped layer arranged on a substrate. The doped layer is n-doped or p-doped. A multiple quantum well is arranged on the doped layer and includes a plurality of adjacent pairs of quantum wells and quantum barriers. An electron blocking layer is arranged on the multiple quantum well. The doped layer, the electron blocking layer, the quantum wells, and all of the quantum barriers except for the last quantum barrier include a first III-nitride alloy. The last quantum barrier includes a second III-nitride alloy that is different from the first III-nitride alloy. The second III-nitride alloy has a bandgap larger than a bandgap of the last quantum well and smaller than a bandgap of the electron blocking layer. An interface between the last quantum barrier and the electron blocking layer exhibits a polarization difference between 0 and 0.012 C/m2.

Abstract: Sulfur species may be processed using an ionic liquid, a deep eutectic solvent, or a combination of both. An example of a method of such processing may include: supplying a first reaction medium including an iodine species and a first catalyst, wherein the first catalyst includes a first deep eutectic solvent, a first ionic liquid, or a first mixture of both; contacting the reaction medium with a first sulfur species, wherein the first sulfur species includes hydrogen sulfide, a sulfur-containing hydrocarbon, or any combination thereof; and reacting the first sulfur species with the reaction medium to produce a second sulfur species, hydrogen iodide, or a combination thereof.

Abstract: Systems and methods for correcting NMR T2 times are disclosed. The method may include creating a plurality of 3D rough surface pore models, where each 3D rough surface pore model includes a rough surface, for each of the plurality determining a pore roughness coefficient (PRC) and a volume; and simulating a first T2 relaxation time based on the 3D rough surface pore model. The method may further include determining a smooth surface pore model, with the same volume as the 3D rough surface pore model, and simulating a second T2 relaxation time based on the smooth surface pore model. The method may still further includes determining a T2 rough surface correction factor based on the first and the second T2 relaxation times, and forming a data pair comprising the pore roughness coefficient (PRC) and the T2 rough surface correction factor; and fitting a T2 correction curve to the data pairs.

Abstract: A composition of matter is provided comprising hierarchically ordered crystalline microporous material having well-defined long-range mesoporous ordering of cubic symmetry. The composition possesses mesopores having walls of crystalline microporous material and a mass of mesostructure between mesopores of crystalline microporous material. Long-range ordering is defined by presence of secondary peaks in an X-ray diffraction (XRD) pattern and/or cubic symmetry observable by microscopy.

Abstract: An electromagnetic (EM) inspection tool for inspecting a pipe that includes a longitudinally extending body having a first end, a second end, and a central longitudinal axis. The EM inspection tool further includes a transmitter disposed proximate the first end and configured to generate an alternating EM field at a first frequency. The EM inspection tool further includes a first far-field receiver plate disposed proximate the second end, wherein the first far-field receiver plate includes a first far-field receiver disposed at a first radial location and a second far-field receiver disposed at a second radial location. The EM inspection tool further includes a first near-field receiver plate disposed circumferentially around the transmitter, wherein the first near-field receiver plate includes a first near-field receiver disposed at the first radial location and a second near-field receiver disposed at the second radial location.