Abstract: The invention discloses an herbal extract of treating liver cancer, wherein a method of producing the herbal extract comprising the steps of: blending Antrodia cinnamomea, Rhinacanthus nasutus and Phellinus linteus and obtaining a mixture, wherein the weight percentages of Antrodia cinnamomea, Rhinacanthus nasutus and Phellinus linteus are 33.4 to 60%, 20 to 33.4% and 20 to 33.4% by weight of the mixture, respectively; soaking the mixture with a 95% ethanol solution with a weight-volume percentage being 50%, followed by extracting at 50 to 80° C.; and concentrating the extracted product to obtain the herbal extract. The invention also discloses a method of treating liver cancer.