Abstract: The present invention relates to a method for mass-producing an antifreeze protein derived from a polar yeast, and more particularly, to a method for mass-producing an antifreeze protein derived from Leucosporidium sp., which is the polar yeast, for synthesizing a recombinant polynucleotide by optimizing and altering a gene, which codes the antifreeze protein derived from the polar yeast, for a yeast expression system, and for expressing same using the yeast expression system.

Abstract: Disclosed is a method of extracting fatty acid methyl esters as the main component of biodiesel from microalgae and producing biodiesel using the extracts. The method includes a process of extracting vegetable oil including triglyceride from microalgae; a process of adding a catalyst-containing alcohol to the extracted vegetable oil and transesterifying the oil while slowly heating the solution to a suitable temperature; a process of, after the completion of the transesterification, cooling the reaction product so as to be separated into crude biodiesel and the byproduct glycerol; a process of separating and washing the upper crude biodiesel layer; and a process of performing gas chromatography to analyze the content and yield of the washed biodiesel. A process for production of microalgae-derived biodiesel is provided and the process is very useful for producing microalgae-derived biodiesel that is more cost-effective than plant-derived biodiesel.

Abstract: The present invention relates to a hyperthermophilic DNA polymerase and a preparation method thereof. The invention provides a novel hyperthermophilic DNA polymerase isolated from a Thermococcus sp. strain, a functional equivalent thereof, a protein having the amino acid sequence thereof, and a preparation method thereof. The DNA polymerase according to the invention is a DNA polymerase, which is hyperthermophilic and has an elongation ability and fidelity higher than those of prior commercial DNA polymerases. Thus, the DNA polymerase according to the invention will be useful in precision analysis, precision diagnosis, identification and the like, which require accurate PCR.