Abstract: A method for concentrating and detecting minute amounts of microscopic substances (especially pathogenic viruses) present in aqueous solutions containing biological materials such as saliva, throat wipes, and fecal suspension at three-digit microliter level volumes by adding basic substance, chelating agent, reducing agent, and protein component to PEG solutions. By combining PEG solution with these reagents and highly sensitive detection technology, it has become possible to detect and monitor microscopic substances such as viruses present in large volumes of biological samples and environmental and food materials easily, rapidly, and sensitively. As a result, it is now possible to contribute to the prevention of virus infection in the medical field and/or public and food safety field, etc.

Abstract: Provided is a method suitable for concentrating the viruses present in the environment, food or biomaterials in fields such as inspections for ensuring public or food safety and medical examinations, and reagents used therein. By adding an optimum concentration of polysaccharide such as glycogen to polyethylene glycol (PEG) and optimizing the concentration of a salt to be added, the viruses suspended in an aqueous solution can be easily, rapidly and stably recovered at a high recovery rate.

Abstract: The present invention relates to a method for easily, rapidly and stably concentrating the viruses present in an aqueous solution at a high recovery rate, by adding a PEG solution containing the optimum concentration of polysaccharide and salt, shortening the settling time before centrifugation and relaxing the centrifugation conditions etc., and reagents used therein. The combined use of the method and the reagents for virus concentration with high-sensitive detection techniques and the reaction reagents used therein, enables easy and rapid detection or monitoring of viruses present in the environment, food materials or biomaterials with high sensitivity, which can contribute to the protection of viral infections in the fields of public safety, food safety and medicine.