Patents Assigned to KUEHNLE AGROSYSTEMS, INC.
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Patent number: 11377675Abstract: The invention pertains to a method for synthesizing a product of interest by culturing a microalgal cell obtained from a subterranean habitat for producing the product of interest. The microalgal cell obtained from a subterranean habitat can be cultured in the dark, in light, in low nutrition, or nutrient rich conditions for at least a portion of production cycle. A combination of these conditions can be used to specifically manipulate a microalgal cell culture to produce a product of interest. The product of interest can be a water-soluble carotenoid, for example, a water-soluble carotenoid produced by culturing an algae belonging to the genus Haematococcus or a capsular exopolysaccharide produced by culturing an algae belonging to the genus Parachlorella. Compositions containing the water-soluble carotenoid, for example, as sunscreen and compositions containing the exopolysaccharide, for example, as moisturizing cream are also described.Type: GrantFiled: January 16, 2020Date of Patent: July 5, 2022Assignee: Kuehnle AgroSystems, Inc.Inventors: Adelheid R. Kuehnle, Robert J. Schurr, Michael C. Perez, Norie Anne B. Nolasco
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Patent number: 11034968Abstract: The invention pertains to a method for synthesizing a product of interest by culturing a microalgal cell producing the product of interest in the dark in a culture medium comprising an organic acid as a fixed carbon source, wherein the microalgal cell is a facultative heterotroph. The product of interest can be a microalgal biomass, a pigment, terpene, recombinant molecule, biogas, or a precursor thereof. In an embodiment, the culture medium comprises urea as a primary source of nitrogen. In one embodiment, the microalgal cell belongs to the order Chlamydomonadales. A method of identifying and isolating a microalgal cell having a preferred characteristic that is suitable for synthesis of a product of interest is also provided, the method comprising identifying and isolating a non-mutagenized or recombinant microalgal cell from a microalgal culture using a fluorescence activated cell sorting technique and/or a phototaxic response.Type: GrantFiled: June 30, 2017Date of Patent: June 15, 2021Assignee: Kuehnle AgroSystems, Inc.Inventors: Robert J. Schurr, Adelheid R. Kuehnle
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Patent number: 9487790Abstract: Various embodiments provide, for example, vectors, expression cassettes, and cells useful for transgenic expression of nucleic acid sequences. In various embodiments, vectors can contain nuclear-based sequences of unicellular photosynthetic bioprocess organisms for the production of food- and feed-stuffs, oils, biofuels, starches, raw materials, pharmaceuticals or fine chemicals.Type: GrantFiled: March 31, 2009Date of Patent: November 8, 2016Assignee: Kuehnle AgroSystems, Inc.Inventors: Michele M. Champagne, Adelheid R. Kuehnle
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Patent number: 9120686Abstract: The present invention relates to improved wastewater treatment systems and methods. In one embodiment, the present invention utilizes microbial consortia and the final effluent of the treated wastewater is remarkably clean, and has low levels of hazardous chemicals, unwanted nutrient values and/or total solids. Advantageously, the wastewater treatment system and method of the present invention reduces the use of chemical compounds in the treatment process. Further, valuable biomasses can be produced from the wastewater using the treatment process of the present invention.Type: GrantFiled: March 13, 2014Date of Patent: September 1, 2015Assignee: Kuehnle AgroSystems, Inc.Inventors: Adelheid R. Kuehnle, Martin Johnson, Robert J. Schurr
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Publication number: 20150001149Abstract: The present invention relates to improved wastewater treatment systems and methods. In one embodiment, the present invention utilizes microbial consortia and the final effluent of the treated wastewater is remarkably clean, and has low levels of hazardous chemicals, unwanted nutrient values and/or total solids. Advantageously, the wastewater treatment system and method of the present invention reduces the use of chemical compounds in the treatment process. Further, valuable biomasses can be produced from the wastewater using the treatment process of the present invention.Type: ApplicationFiled: March 13, 2014Publication date: January 1, 2015Applicant: KUEHNLE AGROSYSTEMS, INC.Inventors: ADELHEID R. KUEHNLE, MARTIN JOHNSON, ROBERT J. SCHURR
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Patent number: 8748161Abstract: The subject invention relates to novel methods for treating microbial biomass and uses thereof. In particular, this invention provides methods for production of lipids using hydrophobic ionic liquid solutions, and subsequent uses of biomass components in food, biofuels, and as chemical precursors. Further, this invention provides methods for recovering the ionic liquids using an antisolvent, thus enabling subsequent reuse of the ionic liquids.Type: GrantFiled: November 24, 2010Date of Patent: June 10, 2014Assignee: Kuehnle AgroSystems, Inc.Inventors: Adelheid R. Kuehnle, Norie Anne B. Nolasco
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Patent number: 8735140Abstract: The present invention relates to compositions and uses of a novel Dunaliella salina HT04 microorganism. In addition, the present invention relates to novel methods for culturing harvesting, preservation, storage, and production of algae seedstock and uses thereof.Type: GrantFiled: January 30, 2012Date of Patent: May 27, 2014Assignee: Kuehnle AgroSystems, Inc.Inventor: Adelheid R. Kuehnle
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Publication number: 20120225472Abstract: The present invention relates to compositions and uses of a novel Dunaliella salina HT04 microorganism. In addition, the present invention relates to novel methods for culturing harvesting, preservation, storage, and production of algae seedstock and uses thereof.Type: ApplicationFiled: January 30, 2012Publication date: September 6, 2012Applicant: KUEHNLE AGROSYSTEMS, INC.Inventor: ADELHEID R. KUEHNLE
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Publication number: 20120094385Abstract: The present invention is directed to Gene Positioning technology for biosynthesis of one or more products of interest via plastid transformation of plants or algae, such as for example tobacco, Lemna, Rhodomonas and Cryptomonas, with pseudogene vectors containing polynucleotides encoding one or more products of interest. The present invention is also directed to transgenic plants or algae, containing pseudogene vectors integrated into a desired locus in the plastid genome, allowing simultaneous expression of multiple transgenes.Type: ApplicationFiled: March 31, 2011Publication date: April 19, 2012Applicant: KUEHNLE AGROSYSTEMS, INC.Inventors: Adelheid R. Kuehnle, Michele Champagne
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Publication number: 20090176272Abstract: Various embodiments provide, for example, vectors, expression cassettes, and cells useful for transgenic expression of nucleic acid sequences. In various embodiments, vectors can contain plastid-based sequences of unicellular photosynthetic bioprocess organisms for the production of food- and feed-stuffs, oils, biofuels, pharmaceuticals or fine chemicals.Type: ApplicationFiled: September 12, 2008Publication date: July 9, 2009Applicant: KUEHNLE AGROSYSTEMS, INC.Inventors: Michele M. Champagne, Adelheid R. Kuehnle
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Patent number: PP34488Abstract: A novel variety of the microalga Haematococcus pluvialis (syn. H. lacustris), named ‘KAS1601-WG7’ is primarily characterized by a yellow and motile cell body during propagation transitioning to a red and motile cell body under pigment accumulation conditions. The cells contain negligible chlorophyll. It has fast growth under heterotrophic cultivation for ease of propagation and shows high carotenogenesis under dark heterotrophic cultivation. The highly pigmented non-encysted cells are attractive for ease of extraction and for high digestibility, including as whole cells, to release a mixture of oils, proteins, pigments, and other components desirable for use in food, feeds, cosmetics and dietary supplements, and excellent storage as biomass.Type: GrantFiled: October 30, 2020Date of Patent: August 9, 2022Assignee: Kuehnle AgroSystems, Inc.Inventor: Robert J. Schurr