Abstract: The present disclosure provides a method of preparing a high density culture of bacteria. The method provides a bioreactor comprising a fermentation tank, a perfusion system, a medium, and a cell separator; preparing an inoculum comprising a bacteria consortia with at least two bacteria species; inoculating the fermentation tank with the inoculum to form a fermentation culture; and growing the bacteria consortia until it has reached a target density of at least about 1012 CFU/mL.
Type:
Application
Filed:
October 25, 2021
Publication date:
December 21, 2023
Applicant:
KULA BIO, INC.
Inventors:
Frederic KENDIRGI, Sung-Yong Harrison YOON