Abstract: An oligopeptide is prepared by use of a vector wherein DNA coding for the intended oligopeptide has been introduced into a plasmid including a leader gene and an alpha-amylase structural gene.
Type:
Grant
Filed:
July 18, 1985
Date of Patent:
June 19, 1990
Assignees:
Kunio Yamane, Daicel Chemical Industries, Ltd., The Calpis Food Industry Co., Ltd., Oji Corn Starch Co., Ltd., Nissan Chemicals Industries, Ltd.
Abstract: This invention relates to DNA consisting of a DNA base sequence coding for the signal peptide: ##STR1## and to DNA containing said DNA base sequence. The DNA base sequence coding for said signal peptide includes, for example; ##STR2## The desired products in cells can be secreted out of cells by the use of a vector containing DNA consisting of the DNA base sequence coding for said signal peptide.
Type:
Grant
Filed:
February 25, 1985
Date of Patent:
September 1, 1987
Assignees:
Kunio Yamane, The Calpis Food Industry Co., Ltd., Daicel Chemical Industries, Ltd., Oji Corn Starch Co., Ltd.
Abstract: This invention relates to DNA consisting of a DNA base sequence coding for the signal amino acid sequence:______________________________________ Met Phe Ala Lys Arg Phe Lys Thr Ser Leu Leu Pro Leu Phe Ala Gly Phe Leu Leu Leu Phe Tyr Leu Val Leu Ala Gly Pro Ala Ala Ala Ser Ala Glu Thr Ala Asn Lys Ser Asn Glu, ______________________________________and to DNA containing such a DNA base sequence. The DNA base sequence coding for said amino acid sequence includes, for example, ______________________________________ ATG TTT GCA AAA CGA TTC AAA ACC TCT TTA CTG CCG TTA TTC GCT GGA TTT TTA TTG CTG TTT TAT TTG GTT CTG GCA GGA CCG GCG GCT GCG AGT GCT GAA ACG GCG AAC AAA TCG AAT GAG. ______________________________________The desired products in cells can be secreted out of cells by the use of a vector containing DNA consisting of the DNA base sequence coding for said signal amino acid sequence.
Type:
Grant
Filed:
January 6, 1984
Date of Patent:
May 5, 1987
Assignees:
Kunio Yamane, The Calpis Food Industry Co., Ltd., Mitsui Toatsu Chemicals, Inc.