Abstract: A method of immunoassaying a component to be measured in a sample containing hemoglobin, which comprises reacting a component to be measured in a sample containing hemoglobin with an antibody capable of binding to the component in the presence of a bile acid derivative different from a bile acid derivative that is inherently contained in the sample; a method of suppressing an interference of hemoglobin in immunoassaying a component to be measured in a sample containing hemoglobin, which comprises reacting a component to be measured in a sample containing hemoglobin with an antibody capable of binding to the component in the presence of a bile acid derivative different from a bile acid derivative that is inherently contained in the sample; a reagent of immunoassay of a component to be measured in a sample containing hemoglobin, which comprises a bile acid derivative, are described.

Abstract: It is to provide a device for sampling feces that enables an accurate and quantitative evaluation by decreasing the variations of feces sampling levels, wherein the confirmation of the presence or absence of feces sampling can be performed hygienically and simply from an outside of a container.

Abstract: A method for measuring cholesterol in low-density lipoprotein contained in a sample, which comprises reacting a sample with (i) a combination of cholesterol ester hydrolase and cholesterol oxidase or (ii) a combination of cholesterol ester hydrolase, an oxidized coenzyme and cholesterol dehydrogenase in the presence of: [a] a polyoxyethylene-polyoxyalkylene alkylaryl ether; [b] one or more surfactants selected from the group consisting of a polyoxyethylene-polyoxyalkylene copolymer, a polyoxyethylene alkenyl ether, a polyoxyethylene branched alkyl ether, and a polyoxyethylene-polyoxyalkylene branched alkyl ether; [c] one or more surfactants selected from the group consisting of a primary amine, a secondary amine, a tertiary amine, and a quaternary ammonium; and [d] a polyanion, and measuring a substance formed or consumed in the reaction.

Abstract: A device for detecting or quantifying influenza viruses in a sample, which comprises a detection region having a human anti-influenza virus nucleoprotein antibody immobilized onto a support, a sample supply region, and a sample-migrating region; and a kit for detecting or quantifying influenza viruses, which comprises a solid phase in which a human anti-influenza virus nucleoprotein antibody is fixed onto a carrier.

Abstract: A method for quantitatively determining cholesterol in high-density lipoprotein in a sample, which comprises: reacting a sample with i) cholesterol esterase and cholesterol oxidase or ii) cholesterol esterase, an oxidized coenzyme and cholesterol dehydrogenase in an aqueous medium comprising i) nonionic surfactant, polyanion and albumin or ii) a combination of a surfactant selected from the group consisting of polyoxyethylene alkylamine or polyoxyethylene alkenylamine and a surfactant selected from the group consisting of polyoxyethylene polycyclic phenyl ether sulfate and an anionic bile acid derivative, and measuring the formed hydrogen peroxide or a reduced coenzyme; and a reagent used therefor.

Abstract: The present invention provides a method, a reagent and a kit for the simple and accurate measurement of HDL cholesterol. The method for the measurement of cholesterol in high-density lipoprotein in a sample comprises reacting the sample with i) cholesterol esterase and cholesterol oxidase or ii) cholesterol esterase, oxidized coenzyme and cholesterol dehydrogenase in an aqueous medium comprising a specific nitrogen-containing surfactant having the structure of amine or ammonium salt and a polyanion, and measuring the formed hydrogen peroxide or reduced coenzyme.

Abstract: A method of immunoassaying a component to be measured in a sample containing hemoglobin, which comprises reacting a component to be measured in a sample containing hemoglobin with an antibody capable of binding to the component in the presence of a bile acid derivative different from a bile acid derivative that is inherently contained in the sample; a method of suppressing an interference of hemoglobin in immunoassaying a component to be measured in a sample containing hemoglobin, which comprises reacting a component to be measured in a sample containing hemoglobin with an antibody capable of binding to the component in the presence of a bile acid derivative different from a bile acid derivative that is inherently contained in the sample; a reagent of immunoassay of a component to be measured in a sample containing hemoglobin, which comprises a bile acid derivative, are described.

Abstract: A method for determination of sdLDL-C, which comprises the steps of: (i) in a reaction solution containing a surfactant which preferentially inhibits the reaction of sdLDL-C with enzymes for cholesterol measurement such as cholesterol ester hydrolase, allowing the enzymes for cholesterol measurement to act on a sample to eliminate HDL-C, VLDL-C, CM-C and LgLDL-C in the sample; (ii) adding a reagent which causes the reaction of sdLDL-C remaining in the reaction solution after the above step (i) to form hydrogen peroxide or reduced coenzyme, and measuring the formed hydrogen peroxide or reduced coenzyme; and (iii) determining the sdLDL-C concentration in the sample from the measurement value in (ii) and a previously prepared calibration curve.

Abstract: The present invention provides a reagent for an immunoassay comprising insoluble carrier particles which can give the values to be determined with high accuracy and reliability, and can be stored for a long time; an immunoassay using the reagent; and a method for keeping the reagent stable.

Abstract: The present invention provides a method, a reagent and a kit for the simple and accurate measurement of HDL cholesterol. The method for the measurement of cholesterol in high-density lipoprotein in a sample comprises reacting the sample with i) cholesterol esterase and cholesterol oxidase or ii) cholesterol esterase, oxidized coenzyme and cholesterol dehydrogenase in an aqueous medium comprising a specific nitrogen-containing surfactant having the structure of amine or ammonium salt and a polyanion, and measuring the formed hydrogen peroxide or reduced coenzyme.

Abstract: The present invention provides a method for a simple and accurate measurement of triglycerides in low-density lipoprotein in a sample comprising performing the following steps sequentially: (i) a step of generating free glycerol by allowing lipoprotein lipase to act on a sample, in an aqueous medium comprising the sample and a specific surfactant such as polyoxyethylene polyoxyalkylene polycyclic phenyl ether; (ii) a step of removing free glycerol present in the reaction solution of the above step (i); (iii) a step of generating free glycerol by allowing lipoprotein lipase to act on the reaction solution from which free glycerol has been removed in step (ii), in the presence of a specific surfactant such as polyoxyethylene polyoxyalkylene alkyl ether; and (iv) a step of measuring free glycerol generated in step (iii), and a kit used for the method.

Abstract: The present invention provides a method for simple and accurate measurement of cholesterol in remnant-like lipoprotein (RLP) (RLP-C) in a sample, which comprises the steps of: (i) eliminating cholesterol in lipoproteins other than RLP by reacting the sample with cholesterol oxidase, or cholesterol dehydrogenase and oxidized coenzyme, in an aqueous medium comprising surfactant A; (ii) reacting the reaction solution from which cholesterol in lipoproteins other than RLP has been eliminated in the above step (i) with cholesterol esterase and cholesterol oxidase, or cholesterol esterase, cholesterol dehydrogenase and oxidized coenzyme, in the presence of surfactant B to form hydrogen peroxide or reduced coenzyme; and (iii) measuring the hydrogen peroxide or reduced coenzyme formed in the above step (ii).

Abstract: The present invention provides a simple method for the stabilization of a peptide in a biological sample and a reagent for the stabilization, a simple method for the preservation of a biological sample comprising a peptide and a reagent for the preservation, and a method for the accurate measurement of a peptide in a biological sample and a reagent for the measurement. Addition of a saccharide to a biological sample enables the stabilization of a peptide in the biological sample, the preservation of the biological sample comprising a peptide in a stable condition and the accurate measurement of a peptide in the biological sample. As the present invention can stabilize a peptide in a biological sample collected in the clinical examination, the peptide as a marker in the biological sample can be measured accurately in the clinical examination.

Abstract: The present invention provides a method, a reagent and a kit for simple and sensitive determination of cholesterol in remnant-like particles without separation of components of a sample. A method for quantitatively determining remnant-like particle cholesterol in a sample, which comprises: in an aqueous medium containing the sample and in the presence of a combination of specific surfactants and a phospholipid-hydrolyzing enzyme, allowing remnant-like particle cholesterol in the sample to react with cholesterol esterase and cholesterol oxidase or cholesterol dehydrogenase (in the presence of oxidized coenzyme); and determining the formed hydrogen peroxide or reduced coenzyme.

Abstract: The present invention relates to a method for diagnosing leukemia, pre-leukemia or aleukemic malignant blood diseases, a method of discriminating leukemia from pre-leukemia or aleukemic malignant blood diseases, a method of discriminating aplastic anemia from myelodysplastic syndrome, a method of diagnosing delayed engraftment of the hematopoietic stem cells after transplantation of the hematopoietic stem cells, and a method of diagnosing the graft versus host disease, each of said methods comprising quantifying stem cell growth factor (SCGF). The present invention also makes it possible to provide an agent for diagnosing leukemia, pre-leukemia or aleukemic malignant blood diseases and an agent for diagnosing delayed engraftment of the hematopoietic stem cells after transplantation of the hematopoietic stem cells or an agent for diagnosing graft versus host disease (GVHD), each containing as an active ingredient an antibody reacting with stem cell growth factor (SCGF).

Abstract: According to the present invention, esterified and free cholesterol in very low-density lipoprotein remnant (hereinafter collectively referred to as very low-density lipoprotein remnant cholesterol) in a sample is determined in an aqueous medium containing the sample in the presence of a combination of (a) a polyoxyethylene-polyoxyalkylene alkylaryl ether and (b) one kind of surfactant selected from the group consisting of a polyoxyethylene-polyoxybutylene copolymer, a polyoxyethylene styrenated-phenyl ether and a polyoxyalkylene long-chain alkyl ether which combination is capable of causing cholesterol esterase and cholesterol oxidase or cholesterol dehydrogenase to act specifically on very low-density lipoprotein remnant cholesterol.

Abstract: The present invention provides a simple and sensitive aqueous method for determining cholesterol in remnant-like particles in a biological sample without the separation of components of the sample. The method comprises contacting the sample with (i) cholesterol esterase, (ii) cholesterol oxidase or cholesterol dehydrogenase, and (iii) phospholipase with a surfactant that inhibits cholesterol oxidase or cholesterol dehydrogenase in lipoproteins other than remnant-like particles in the presence of oxygen or an oxidized coenzyme. Formed hydrogen peroxide or reduced coenzyme is measured and correlated to a quantitative determination of cholesterol in remnant-like particles.

Abstract: According to the present invention, a device for measuring an object to be measured in a sample which comprises a support, sample addition site (S) and a detection site (Q), said sample addition site and said detection site being on the support, said support allowing the object to be measured to move by the capillary flow of a developing liquid, said detection site having a piezoelectric vibrator sandwiched between two electrodes, said piezoelectric vibrator having a trapper A, (c1) immobilized thereon, or an analogue of the object to be measured (c1?) immobilized thereon, and said support further comprising a binder retaining site(BR) where a binder (b1) is retained therein so that it is movable by the capillary flow of the developing liquid. Furthermore, the present invention provides a method for quantitatively determining an object to be measured in a sample using the device.

Abstract: The present invention provides an agglutination immunoassay, wherein the agglutination of insoluble carrier particles such as latex are stabilized and uniformized to give good reproducibility, and a reagent therefor. In the agglutination immunoassay which comprises allowing an antigenic substance in a sample to bind to insoluble carrier particles carrying substantially neither antigens nor antibodies thereon, and allowing an antibody or an antibody complex which reacts specifically to the antigenic substance to bind to the antigenic substance to give a selective agglutination of the insoluble carrier particles, a homopolymer prepared by polymerization of a monomer such as 2-methacryloyloxyethyl phosphorylcholine having a phosphorylcholine group and a vinyl group, or a copolymer prepared by polymerization of a monomer having a phosphorylcholine group and a vinyl group, with a monomer having a vinyl group such as n-butyl methacrylate is used.

Abstract: A method for quantitatively determining cholesterol in high-density lipoprotein in a sample, which comprises: reacting a sample with i) cholesterol esterase and cholesterol oxidase or ii) cholesterol esterase, an oxidized coenzyme and cholesterol dehydrogenase in an aqueous medium comprising i) nonionic surfactant, polyanion and albumin or ii) a combination of a surfactant selected from the group consisting of polyoxyethylene alkylamine or polyoxyethylene alkenylamine and a surfactant selected from the group consisting of polyoxyethylene polycyclic phenyl ether sulfate and an anionic bile acid derivative, and measuring the formed hydrogen peroxide or a reduced coenzyme; and a reagent used therefor.