Abstract: The present invention provides a method for the quantification of NO in a blood sample comprising the steps of collecting a blood sample, dividing said collected blood sample into three blood subsamples, chemically treating one blood subsample with an antioxidant to at least partly reduce free radicals of said blood subsample, performing EPR measurement of said blood subsamples, performing a first comparison of said chemically treated blood subsample EPR measurement with the EPR measurement of a non-chemically treated blood sub-sample thereby obtaining a first comparison result and performing a second comparison of this result with an EPR measurement of the remaining blood subsample in order to eliminate background signals.

Abstract: The present invention provides a method for the quantification of NO in a blood sample comprising the steps of collecting a blood sample, dividing said collected blood sample into three blood subsamples, chemically treating one blood subsample with an antioxidant to at least partly reduce free radicals of said blood subsample, performing EPR measurement of said blood subsamples, performing a first comparison of said chemically treated blood subsample EPR measurement with the EPR measurement of a non-chemically treated blood sub-sample thereby obtaining a first comparison result and performing a second comparison of this result with an EPR measurement of the remaining blood subsample in order to eliminate background signals.

Abstract: A compound useful for treating subjects in need of therapy involving sigma receptors or for alleviation of affects resulting from drug abuse having the general formula I in which R1 can be a radical of an optionally substituted C-4 to C-7 N-containing heterocycle such as, for example, radicals of optionally substituted piperidines, optionally substituted piperazines, optionally substituted tetrahydropyridines, optionally substituted azepanes, tertiary amines (cyclic or acyclic), isoindoline-1,3-dione, or optionally substituted tetrahydroisoquinolones (aromatically substituted): R2,3,4,5,6 can each independently be any one or combinations of the following moieties, cyano, nitro, acyl, alkyl, amido, azido, isothiocyanate, isocyanate optionally substituted anilino, halogens, ethers, sulfonamides, thioacyl, nitro, aromatic, heterocyclic, olefinic, acetylene, deuterium, or tritium; Y can be either CH, CH2, O, S, OCH2, N—R, N—Ar, C—R, C—Ar; Z can be either H, O, S, S—R or NR.

Abstract: Compounds having the general formula II, III, or IV wherein R1 can be a radical of an optionally substituted C-4 to C-7 N-containing heterocycle or a radical of an optionally substituted cyclic or acyclic tertiary amine or isoindoline-1,3-dione: R2,3,4,5,6 can each independently be any one or combinations of the following moieties, cyano, nitro, acyl, alkyl, amido, azido, isothiocyanate, isocyanate, optionally substituted anilino, halogens, ethers, sulfonamides, thioacyl, nitro, aromatic, heterocyclic, olefinic, acetylene, deuterium, or tritium; Y can be either CH, CH2, O, S, OCH2, N—R, N—Ar, C—R, C—Ar; Z can be either H, O, S, S—R or NR.

Abstract: The present invention is directed to methods and compositions for TnpI-mediated genetic engineering using the Bacillus thuringiensis recombinase TnpI and the TnpI recombination substrates TRT or TRT?, and variants thereof. In particular, the invention relates to TRT or TRT? sequences, and variants thereof, vectors, cells and kits useful for TnpI-mediated genetic recombination, as well as methods for the use of TRT or TRT? sequences for TnpI-mediated genetic engineering.