Abstract: Provided herein, inter alia, are compositions and methods for culturing mammalian cells. In certain aspects, the composition is a medium containing one or more of a lithium ion source, one or more fatty acids, and/or ethanol. Use of any of the cell culture media described herein to culture cells that have been genetically engineered to produce one or more recombinant polypeptides (for example, antibodies) can result in increased titers, a more favorable glycosylation profile, and/or modulated (e.g. decreased) amounts of high and low molecular weight species, and/or modulated (e.g. decreased) amounts of acidic or basic charge variants, compared to cells cultured in a medium that does not contain one or more of a lithium ion source, one or more fatty acids, and/or ethanol.
Type:
Grant
Filed:
April 26, 2017
Date of Patent:
July 7, 2020
Assignee:
LA JOLLA BIOLOGICS, INC.
Inventors:
Christopher T. Leber, Michael W.Y. Shen, Yiwen Tao, Hugh Eugene Murray, IV
Abstract: The invention relates to a process for separating acetylated proteins from unacetylated proteins. In particular, the invention relates to a process of using multimodal chromatography to separate acetylated proteins from unacetylated proteins. In the prior art, multimodal chromatography is used to isolate antibodies. The invention found that multimodal chromatography can be used in the separation of acetylated proteins from unacetylated proteins.
Type:
Application
Filed:
April 18, 2014
Publication date:
August 14, 2014
Applicants:
TANVEX BIOLOGICS CORP., LA JOLLA BIOLOGICS, INC.
Inventors:
Kuo-Ming YU, Judy Huei-chuan CHOU, Jennifer Renee HOPP