Abstract: Methods for single molecule localization microscopy may use a patterned illumination over the field of view. The patterned illumination may be dynamically adapted to the approximate positions of the detected fluorescent molecule emitters, allowing for increased signal-to-background for their single molecule localization.

Abstract: A radiation field measuring device for the characterization of a radiation comprises a detector device and a reconstruction device. The detector device may have at least one detector camera, which contains at least one detector array arranged for the image recording of scattered radiation in a multiplicity of lateral directions that deviate from the longitudinal direction. The reconstruction device may be configured for the tomographic reconstruction of a field density of the scattered radiation in the radiation field.

Abstract: The present application discloses a light sheet microscope for imaging biological materials. The microscope uses a plurality of light beams, focused to an overlapping line to excite a fluorescent material within the biological sample. The laser-induced fluorescence image is then analyzed and displayed.

Abstract: The present application discloses a light sheet microscope for imaging biological materials. The microscope uses a plurality of light beams, focused to an overlapping line to excite a fluorescent material within the biological sample. The laser-induced fluorescence image is then analyzed and displayed.

Abstract: The present application discloses a light sheet microscope for imaging biological materials. The microscope uses a plurality of light beams, focused to an overlapping line to excite a fluorescent material within the biological sample. The laser-induced fluorescence image is then analyzed and displayed.

Abstract: A radiation field measuring device for the characterization of a radiation comprises a detector device and a reconstruction device. The detector device may have at least one detector camera, which contains at least one detector array arranged for the image recording of scattered radiation in a multiplicity of lateral directions that deviate from the longitudinal direction. The reconstruction device may be configured for the tomographic reconstruction of a field density of the scattered radiation in the radiation field.

Abstract: A radiation field measuring device for the characterization of a radiation field is disclosed. The measuring device may include a detector device and a reconstruction device. The detector device may have at least one detector camera, which contains at least one detector array arranged for the image recording of scattered radiation in a multiplicity of lateral directions that deviate from the longitudinal direction. The reconstruction device may be configured for the tomographic reconstruction of a field density of the scattered radiation in the radiation field.

Abstract: The invention relates to a device for examining samples (1) in a liquid (5), comprising a movable shaft (2), to which the sample (1) is fastened, and a cuvette (4), wherein the device further comprises a bath (3), which surrounds the movable shaft (2), and wherein the bath (3) is fillable with the liquid (5), the movable shaft (2) is configured to receive the sample (1) at the upper side (24) thereof, the movable shaft (2) reaches into the cuvette (4) from below, wherein said cuvette is open at least toward the bottom and configured to be immersed into the liquid (5) in the bath (3) with the underside thereof, and, moreover, means are provided to generate a pressure difference between the interior of the cuvette (4) and the region outside of the cuvette (4) such that the fill level (21) of the liquid (5) in the cuvette (4) is adjustable. Moreover, the invention relates to a method for examining samples (1) in a liquid (5).

Abstract: The invention relates to a laser assembly (100) having a laser (L) for generating primary laser pulses (1), beam splitting optics (15) for splitting a primary laser pulse into a plurality of temporally staggered sub-pulses, and having focusing optics (17-19) for focusing the sub-pulses in or on an object (20) so that every sub-pulse is focused in a separate focus volume (F). The invention is characterized in that the mutual spatial and/or temporal relationship of the focus volumes (F) of the sub-pulses originating from a common primary laser pulse is variably adjustable. The invention also relates to a corresponding method.

Abstract: The invention relates to a laser assembly (100) having a laser (L) for generating primary laser pulses (1), beam splitting optics (15) for splitting a primary laser pulse into a plurality of temporally staggered sub-pulses, and having focusing optics (17-19) for focusing the sub-pulses in or on an object (20) so that every sub-pulse is focused in a separate focus volume (F). The invention is characterized in that the mutual spatial and/or temporal relationship of the focus volumes (F) of the sub-pulses originating from a common primary laser pulse is variably adjustable. The invention also relates to a corresponding method.

Abstract: The subject matter of the invention is a readout method performed by stripe scanning planar objects with substances emitting fluorescence radiation, a detector being provided and said fluorescence radiation being imaged onto said detector, said detector comprising at least one row of N-linearly disposed single detector segments, with the scanning direction being rotated by angle ? with respect to the at least one detector row.

Abstract: The subject matter of the invention is a beam splitter device having two spaced apart parallel highly reflecting mirrors, between which there is disposed a semireflecting mirror which is spaced a different distance from the at least one highly reflecting mirror, said semireflecting mirror having at least one portion configured to form a full reflector or an antireflector, the semireflecting mirror being slidably disposed between the highly reflecting mirrors and a laser-scanning microscope as well.

Abstract: A base member for a biochip, e.g., a chip for analyzing DNA, RNA, proteins or cells, the base member (1), which is shaped like a board, being provided with a plurality of discrete elevations (2) extending from the upper side (1a) to the underside and having, in the region of their lower end, an active surface (3) for receiving the probe material.

Abstract: The subject matter of the invention is a beam splitter device having two spaced apart parallel highly reflecting mirrors, between which there is disposed a semireflecting mirror which is spaced a different distance from the at least one highly reflecting mirror, said semireflecting mirror having at least one portion configured to form a full reflector or an antireflector, the semireflecting mirror being slidably disposed between the highly reflecting mirrors and a laser-scanning microscope as well.