Patents Assigned to Labcyte Inc.
  • Patent number: 10325768
    Abstract: Focused acoustic radiation, referred to as tonebursts, are applied to a volume of liquid to generate a set of droplets. In one embodiment, a first toneburst is applied to temporarily raise a mound or protuberance on a free surface of the fluid. After the mound has reached a certain state, at least two additional toneburst can be applied to the protuberance to sequentially eject multiple bursts of multiple droplets. In one embodiment, the state of the mound can be maintained by a sustained acoustic signal, during which time multiple additional tonebursts can be applied to sequentially eject multiple bursts of multiple droplets from the mound.
    Type: Grant
    Filed: September 2, 2016
    Date of Patent: June 18, 2019
    Assignee: LABCYTE INC.
    Inventor: Richard G. Stearns
  • Publication number: 20190157060
    Abstract: A system and method are provided for loading a sample into an analytical instrument using acoustic droplet ejection (“ADE”) in combination with a continuous flow sampling probe. An acoustic droplet ejector is used to eject small droplets of a fluid sample containing an analyte into the sampling tip of a continuous flow sampling probe, where the acoustically ejected droplet combines with a continuous, circulating flow stream of solvent within the flow probe. Fluid circulation within the probe transports the sample through a sample transport capillary to an outlet that directs the analyte away from the probe to an analytical instrument, e.g., a device that detects the presence, concentration quantity, and/or identity of the analyte. When the analytical instrument is a mass spectrometer or other type of device requiring the analyte to be in ionized form, the exiting droplets pass through an ionization region, e.g.
    Type: Application
    Filed: November 21, 2018
    Publication date: May 23, 2019
    Applicants: Labcyte, Inc., DH Technologies Development Pte. Ltd.
    Inventors: Sammy Datwani, Donald W. Arnold, Lucien P. Ghislain, Chang Liu, Thomas Covey
  • Publication number: 20190157061
    Abstract: A system and method are provided for loading a sample into an analytical instrument using acoustic droplet ejection (“ADE”) in combination with a continuous flow sampling probe. An acoustic droplet ejector is used to eject small droplets of a fluid sample containing an analyte into the sampling tip of a continuous flow sampling probe, where the acoustically ejected droplet combines with a continuous, circulating flow stream of solvent within the flow probe. Fluid circulation within the probe transports the sample through a sample transport capillary to an outlet that directs the analyte away from the probe to an analytical instrument, e.g., a device that detects the presence, concentration quantity, and/or identity of the analyte. When the analytical instrument is a mass spectrometer or other type of device requiring the analyte to be in ionized form, the exiting droplets pass through an ionization region, e.g.
    Type: Application
    Filed: November 21, 2018
    Publication date: May 23, 2019
    Applicant: Labcyte, Inc.
    Inventors: Sammy Datwani, Lucien P. Ghislain
  • Patent number: 10156499
    Abstract: Focused acoustic radiation, referred to as tonebursts, are applied to a volume of liquid to generate a set of droplets. The droplets generated are substantially smaller in scale than the focal spot size of the acoustic beam (e.g., the frequency at which the acoustic transducer operates). Further, the droplets have trajectories that are substantially in the direction of the acoustic beam propagation direction. In one embodiment, a first toneburst is applied to temporarily raise a protuberance on a free surface of the fluid. After the protuberance has reached a certain state, a second toneburst is applied to the protuberance to break it into very small droplets. In one embodiment, the state of the protuberance at which the second toneburst is supplied is the time period shortly after the protuberance reaches its maximum height but before the protuberance recedes back into the volume of fluid.
    Type: Grant
    Filed: September 30, 2013
    Date of Patent: December 18, 2018
    Assignee: Labcyte Inc.
    Inventor: Richard G. Stearns
  • Patent number: 10118186
    Abstract: A system for fluid transport is provided where a quantity of fluid is held in a reservoir. A droplet generator is employed to generate droplets from the fluid, for example a nozzle-based system or a nozzleless system such as an acoustic ejection system. A generated droplet has a trajectory whereby it arrives at a target. A circuit is used to modify one or more characteristics of the generated droplet in a way which increases the likelihood that the droplet will not splash or bounce when it arrives at the target. The circuit may in different embodiments control the speed of the droplet or the Weber number of the droplet. The circuit may create an electric field in an area of space where the droplet passes. The circuit may charge the droplet by causing it to contact ions.
    Type: Grant
    Filed: January 18, 2017
    Date of Patent: November 6, 2018
    Assignee: Labcyte Inc.
    Inventors: Richard G. Stearns, Stephen J. Hinkson
  • Patent number: 10112212
    Abstract: Devices and methods are provided for ejecting a droplet from a reservoir using focused acoustic radiation having a plurality of nonsimultaneous and discrete frequency ranges. Such frequency ranges may be used to control droplet volume and/or velocity. Optionally, satellite fluid ejection from the reservoir is suppressed.
    Type: Grant
    Filed: April 8, 2004
    Date of Patent: October 30, 2018
    Assignee: Labcyte Inc.
    Inventors: Richard G. Stearns, Mitchell W. Mutz, Richard N. Ellson
  • Patent number: 10016757
    Abstract: Sample container for holding and transferring a liquid sample and method thereof. The sample container includes an inlet configured to allow a liquid sample to enter a sample container, and an outlet configured to allow one or more droplets of the liquid sample to exit the sample container by one or more acoustic ejections respectively. The inlet and the outlet are in different locations.
    Type: Grant
    Filed: April 26, 2012
    Date of Patent: July 10, 2018
    Assignee: Labcyte Inc.
    Inventors: Richard N. Ellson, Joseph D. Olechno, Robert G. Kuimelis
  • Patent number: 9939352
    Abstract: A method and system are provided for detecting the concentration of an analyte in a fluid sample. The method and system involve analysis of a volatilized, ionized fluid sample using a mass spectrometer or other ionic analyte detection device that provides a signal proportional in intensity to the quantity of ionized analyte detected. The improvement involves replacement of a necessary non-analyte component in the fluid sample with a substitute component that serves the same purpose as the original component but is either more volatile than the original component and/or the analyte or undergoes a reaction to provide lower molecular weight reaction products, and results in an increased intensity in signal and signal-to-noise ratio. Acoustic fluid ejection is a preferred method of generating nanoliter-sized droplets of fluid sample that are then volatilized, ionized, and analyzed.
    Type: Grant
    Filed: October 19, 2015
    Date of Patent: April 10, 2018
    Assignee: LABCYTE, INC.
    Inventors: Richard N. Ellson, Richard G. Stearns, Joseph D. Olechno, Ian Sinclair, Jonathan Wingfield
  • Patent number: 9920352
    Abstract: Method and system for monitoring for a change in the amount and/or concentration of a pathogen in a pathogenic fluid. The method includes providing a pathogen-impermeable enclosure enclosing the pathogenic fluid, wherein the pathogenic fluid includes a pathogen and a carrier fluid. Additionally, the method includes acoustically monitoring for a change in the amount and/or concentration of the pathogen enclosed in the pathogen-impermeable enclosure.
    Type: Grant
    Filed: April 8, 2013
    Date of Patent: March 20, 2018
    Assignee: Labcyte Inc.
    Inventors: Mitchell W. Mutz, Richard N. Ellson
  • Patent number: 9908133
    Abstract: The invention provides apparatuses and methods for acoustically ejecting the fluid from a reservoir contained in or disposed on a substrate. The reservoir has a portion adapted to contain a fluid, and an acoustic radiation generator is positioned in acoustic coupling relationship to the reservoir. Acoustic radiation generated by the acoustic radiation generator is transmitted through at least the portion of the reservoir to an analyzer. The analyzer is capable of determining the energy level of the transmitted acoustic radiation and raising the energy level of subsequent pulses to a level sufficient to eject fluid droplets from the reservoir. The invention is particularly suited for delivering fluid from a plurality of reservoirs in an accurate and efficient manner.
    Type: Grant
    Filed: November 18, 2015
    Date of Patent: March 6, 2018
    Assignee: Labcyte Inc.
    Inventors: Richard G. Stearns, Shehrzad Ahmed Qureshi
  • Patent number: 9861987
    Abstract: Provided herein is generally tubular container, preferably including a plurality of reservoirs defined therein. The container can be adapted for acoustic ejection of a fluid disposed within at least one of the reservoirs of the plurality of reservoirs. Alternatively, the container can be adapted for extraction of a fluid disposed within at least one of the reservoirs of the plurality of reservoirs using a non-acoustic liquid handling method.
    Type: Grant
    Filed: January 12, 2015
    Date of Patent: January 9, 2018
    Assignee: LABCYTE INC.
    Inventor: Richard N. Ellson
  • Patent number: 9610577
    Abstract: In an aspect of this invention, a closure for a well plate is provided which has a reservoir. The closure has openings through which acoustic ejection of fluid droplets can take place without removing the closure. The reservoirs in the closure may help to maintain acceptable levels of solvent in the wells of the well plate despite the evaporation which may occur during the course of ejection.
    Type: Grant
    Filed: October 15, 2015
    Date of Patent: April 4, 2017
    Assignee: LABCYTE INC.
    Inventor: Richard N. Ellson
  • Patent number: 9586215
    Abstract: A system for fluid transport is provided where a quantity of fluid is held in a reservoir. A droplet generator is employed to generate droplets from the fluid, for example a nozzle-based system or a nozzleless system such as an acoustic ejection system. A generated droplet has a trajectory whereby it arrives at a target. A circuit is used to modify one or more characteristics of the generated droplet in a way which increases the likelihood that the droplet will not splash or bounce when it arrives at the target. The circuit may in different embodiments control the speed of the droplet or the Weber number of the droplet. The circuit may create an electric field in an area of space where the droplet passes. The circuit may charge the droplet by causing it to contact ions.
    Type: Grant
    Filed: January 28, 2013
    Date of Patent: March 7, 2017
    Assignee: Labcyte Inc.
    Inventors: Richard G. Stearns, Stephen J. Hinkson
  • Patent number: 9221250
    Abstract: The invention provides apparatuses and methods for acoustically ejecting the fluid from a reservoir contained in or disposed on a substrate. The reservoir has a portion adapted to contain a fluid, and an acoustic radiation generator is positioned in acoustic coupling relationship to the reservoir. Acoustic radiation generated by the acoustic radiation generator is transmitted through at least the portion of the reservoir to an analyzer. The analyzer is capable of determining the energy level of the transmitted acoustic radiation and raising the energy level of subsequent pulses to a level sufficient to eject fluid droplets from the reservoir. The invention is particularly suited for delivering fluid from a plurality of reservoirs in an accurate and efficient manner.
    Type: Grant
    Filed: May 20, 2014
    Date of Patent: December 29, 2015
    Assignee: LABCYTE INC.
    Inventors: Richard G. Stearns, Shehrzad Ahmed Qureshi
  • Patent number: 9192932
    Abstract: In an aspect of this invention, a closure for a well plate is provided which has a reservoir. The closure has openings through which acoustic ejection of fluid droplets can take place without removing the closure. The reservoirs in the closure may help to maintain acceptable levels of solvent in the wells of the well plate despite the evaporation which may occur during the course of ejection.
    Type: Grant
    Filed: June 20, 2008
    Date of Patent: November 24, 2015
    Assignee: LABCYTE INC.
    Inventor: Richard N. Ellson
  • Publication number: 20140340441
    Abstract: The invention provides apparatuses and methods for acoustically ejecting the fluid from a reservoir contained in or disposed on a substrate. The reservoir has a portion adapted to contain a fluid, and an acoustic radiation generator is positioned in acoustic coupling relationship to the reservoir. Acoustic radiation generated by the acoustic radiation generator is transmitted through at least the portion of the reservoir to an analyzer. The analyzer is capable of determining the energy level of the transmitted acoustic radiation and raising the energy level of subsequent poises to a level sufficient to eject fluid droplets from the reservoir. The invention is particularly suited for delivering fluid from a plurality of reservoirs in an accurate and efficient manner.
    Type: Application
    Filed: May 20, 2014
    Publication date: November 20, 2014
    Applicant: Labcyte Inc.
    Inventors: Richard G. Stearns, Shehrzad Ahmed Qureshi
  • Patent number: 8882226
    Abstract: To ejecting a droplet from a reservoir, the reservoir holding a fluid is moved with respect to an acoustic ejector. As the reservoir and ejector move closer together, the acoustic ejector sends one or more interrogation pulses towards the reservoir. Based on the interrogation pulses, the system determines when the movement of the reservoir has placed a free surface of the fluid in a position where a droplet can be ejected.
    Type: Grant
    Filed: September 3, 2013
    Date of Patent: November 11, 2014
    Assignee: Labcyte Inc.
    Inventor: Richard N. Ellson
  • Patent number: 8770691
    Abstract: The invention provides apparatuses and methods for acoustically ejecting the fluid from a reservoir contained in or disposed on a substrate. The reservoir has a portion adapted to contain a fluid, and an acoustic radiation generator is positioned in acoustic coupling relationship to the reservoir. Acoustic radiation generated by the acoustic radiation generator is transmitted through at least the portion of the reservoir to an analyzer. The analyzer is capable of determining the energy level of the transmitted acoustic radiation and raising the energy level of subsequent pulses to a level sufficient to eject fluid droplets from the reservoir. The invention is particularly suited for delivering fluid from a plurality of reservoirs in an accurate and efficient manner.
    Type: Grant
    Filed: March 10, 2010
    Date of Patent: July 8, 2014
    Assignee: Labcyte Inc.
    Inventors: Richard Stearns, Shehrzad Ahmed Qureshi
  • Publication number: 20140092156
    Abstract: To ejecting a droplet from a reservoir, the reservoir holding a fluid is moved with respect to an acoustic ejector. As the reservoir and ejector move closer together, the acoustic ejector sends one or more interrogation pulses towards the reservoir. Based on the interrogation pulses, the system determines when the movement of the reservoir has placed a free surface of the fluid in a position where a droplet can be ejected.
    Type: Application
    Filed: September 3, 2013
    Publication date: April 3, 2014
    Applicant: Labcyte Inc.
    Inventor: Richard N. Ellson
  • Patent number: 8544976
    Abstract: To ejecting a droplet from a reservoir, the reservoir holding a fluid is moved with respect to an acoustic ejector. As the reservoir and ejector move closer together, the acoustic ejector sends one or more interrogation pulses towards the reservoir. Based on the interrogation pulses, the system determines when the movement of the reservoir has placed a free surface of the fluid in a position where a droplet can be ejected.
    Type: Grant
    Filed: January 26, 2011
    Date of Patent: October 1, 2013
    Assignee: Labcyte Inc.
    Inventor: Richard N. Ellson