Abstract: The invention relates to a process for the determination of serum lipoproteins by an immuno-enzymatic method. The process of the invention consists in reacting a specific antibody of the apoprotein whose amount is to be measured, fixed on a support, with, on the one hand, the compound for coupling the corresponding lipoprotein with an enzyme and, on the other hand, the analyzed sample. The amount of enzyme fixed is then an inverse function of the apoprotein content of the sample. The support with the products which are fixed thereto are separated from the rest of the reagents and the activity of the enzyme of the support is measured which is compared with standard measurements.The process is useful for serum analyses, particularly for early detection of cardiovascular disease risks.
Abstract: The invention relates to methods for measuring cholesterol of various fractions of seric lipoproteins, the methods comprising the separation of said fractions of lipoproteins by means of a lectin capable of precipitating the LDL and VLDL fractions, then measuring the amount of cholesterol in the precipitate and in the remaining solution.