Abstract: The invention relates to composition comprising an L3 and/or an L5 source and optionally an adjuvant for the preparation of a medicine for the treatment or prevention of a parasitic disease and to its diagnostic use of said parasitic disease.
Type:
Grant
Filed:
May 11, 2012
Date of Patent:
March 31, 2020
Assignee:
LABORATORIES LETI S.L.
Inventors:
Carlos Alonso-Bedate, Manuel Soto-Alvarez, Laura Ramírez García
Abstract: Chimeric gene formed by the DNA sequences that encode the antigenic determinants of four proteins of L. infantum, useful for the serological diagnosis of canine Leishmaniosis and protein obtained, that consists of the prior employment of a cloning strategy. The patent describes the intermediate products generated during the process. A clone is achieved expressed in the protein rLiPO-Ct-Q (pPQI). To this initial vector, by means of the use of suitable restriction targets, DNA fragments are sequentially added that are encoded in other proteins and after each cloning step the correct orientation of each one of the inserts reduces the size of the expression products, the complete nucleotide sequence of the final pPQV clone being determined. A polypeptide is obtained with a molecular mass of 38 kD and with an isoelectric point of 7.37.
Type:
Grant
Filed:
June 29, 2005
Date of Patent:
March 13, 2007
Assignee:
Laboratories Leti, S.L. Unipersonal
Inventors:
Carlos Alonso Bedate, Jose Maria Requena Rolania, Manuel Soto Alvarez
Abstract: Chimeric gene formed by the DNA sequences that encode the antigenic determinants of four proteins of L. infantum, useful for the serological diagnosis of canine Leishmaniosis and protein obtained, that consists of the prior employment of a cloning strategy. The patent describes the intermediate products generated during the process. A clone is achieved expressed in the protein rLiPO-Ct-Q (pPQI). To this initial vector, by means of the use of suitable restriction targets, DNA fragments are sequentially added that are encoded in other proteins and after each cloning step the correct orientation of each one of the inserts reduces the size of the expression products, the complete nucleotide sequence of the final pPQV clone being determined. A polypeptide is obtained with a molecular mass of 38 kD and with an isoelectric point of 7.37.
Type:
Application
Filed:
June 29, 2005
Publication date:
December 29, 2005
Applicant:
LABORATORIES LETI, S.L. UNIPERSONAL
Inventors:
Carlos Bedate, Jose Maria Rolania, Manuel Alvarez
Abstract: Chimeric gene formed by the DNA sequences that encode the antigenic determinants of four proteins of L. infantum, useful for the serological diagnosis of canine Leishmaniosis and protein obtained, that consists of the prior employment of a cloning strategy. A clone is obtained which expresses the protein rLiPO-Ct-Q (pPQI). To this initial vector, by means of the use of suitable restriction targets, DNA fragments are sequentially added that are encoded in other proteins and after each cloning step the correct orientation of each one of the inserts reduces the size of the expression products, the complete nucleotide sequence of the final pPQV clone being determined. A chimeric polypeptide encoded by the chimeric gene is obtained with a molecular mass of 38 kD and with an isoelectric point of 7.37. This chimeric polypeptide is useful for preventing and/or treating Leishmaniosis in animals or humans.
Type:
Grant
Filed:
September 9, 2002
Date of Patent:
November 8, 2005
Assignee:
Laboratories Leti S.L. Unipersonal
Inventors:
Carlos Alonso Bedate, Jose Maria Requena Rolania, Manuel Soto Alvarez