Abstract: The present invention refers to a method for producing a human insulin analogue with high efficiency and excellent yield, by means of a biotechnological process comprising transformation of a Pichia pastoris yeast strain. In particular, the invention refers to a biotechnological process for obtaining aspart insulin.

Abstract: Methylotrophic recombinant yeast strain producing human insulin precursor, the strain comprising, in its genome, a copy of a first DNA construction and a second DNA construction, wherein the constructions are capably of directing the expression and secretion of human insulin precursor of the formula B(1-30)-Y1-Y2-A(1-21), wherein Y1 is lysine or arginine; Y2 is lysine or arginine; B(1-30) is the B peptide of the human insulin; and B(1-21) is the A peptide of human insulin, and wherein the strain is yeast Pichia Pastoris. DNA constructions and method for obtaining the strain are also provided.

Abstract: The present invention relates to spironolactone contain compositions for application to an area of human skin afflicted with acne, seborrheic condition or hirsutism. N,N-dialkyl lauramides are used as permeation enhancers.